You are here
Clinical and biochemical characteristics of reparative osteogenesis at osteosynthesis of thigh bones comminuted fractures in dogs using Collapan
The objective - to determine clinical and biochemical criteria of reparative osteogenesis in dogs with comminuted fractures of thigh bone on condition of substituting of bone defects by osteotropic composite Collapan L.
Materials and methods of the research. Dogs with comminuted diaphyseal fractures of thigh bone were divided into control (n = 7) and experimental group (n = 7). In the experimental group after the imposition of a plate the defect was additionally replaced by granules "Collapan L".
Results of the research and discussion. In dogs with fractures of thigh bones set dynamics of content in blood markers of connective tissue molecules of average weight, soluble fibrin, fibrinases, nitric oxide and glucose.
The content of total hexose increased to 3rd day in the experimental group in 1.2 times, and in control in 1.3 times (р<0,001) compared with the preoperative period. This was due to an increase (р<0,05) content of glycoprotein (GP) to 0,98±0,07 g / l in the experimental and 1,0±0,08 g / l in the control group. After 7 days, in the control group there was a decrease in the concentration of G 1.3 and GP 1.4 times (р<0,05). However, in the experimental group contents GP remained elevated. That is, after the application of Collapan phase of inflammatory resorption slightly elongated.
The concentration of molecules middle mass (MMM) at fractures of thigh bone was increased in 1.4 times (р<0,001). This is connected with catabolic processes due to injury and the inflammatory response. In the dogs of the control group after the 60th day, the second peak observed increasing of their content in the blood to a level of 0,77±0,02 g / l (р<0,001). This is due to the larger volume resorption of bone regenerate in control dogs.
In dogs with fractures of the thigh bone concentration of soluble fibrin (SF) was in 1.7 times (р<0,05) more than in clinically healthy animals. In dogs of the control group concentration of SF continued to increase on the 3rd day to 31,7±3,3 mg%, which was in 1.4 times higher (р<0,05) than in research group. This is because in the experimental animals bone defect was filled with Collapan, which contributed to the mechanical stop of bleeding. In the control group on the 7th day, the SF concentration tended to decrease, and in the experimental increased to 27,2±3,1 mg%. Such strengthening of trombinemia most likely is due to the reaction of cattle on collagen, which is a part of Collapan.
In dogs with fractures of the thigh bone, FXIII activity increased to 122,5 ± 5,8%, (р<0,01). On the third day after osteosynthesis tendency to further increase of its activity in experimental dogs was marked, while in the control it, on the contrary, decreased in 1.3 times (р<0,01). On the 7th and 14th day, its activity remained high (р<0,05) in both groups, with no significant difference between them, followed by a tendency to normalization, starting from the 30th day after osteosynthesis.
Consequently, the filling of the bone defect with composite promotes mechanical stop of bleeding, accompanied by less expenditure of FXIII to stabilize the blood clot.
In dogs with fractures of thigh bone the level of nitric oxide decreased to the 7th day. In the control group on the 30th day, the decrease in its level was noted in 1.5 times (р<0,05). In the experimental group the level of nitric oxide did not differ from the rate of clinically healthy dogs to the 60th day. That is, use of Collapan indirectly reduces the level of endothelial dysfunction at reparative process due to smaller changes in local hemostasis at osteosynthesis.
The level of glucose in the blood serum of dogs with fractures of the thigh bone increased to 6,92 ± 0,27 mmol / l, which is in 1.3 times higher (р<0,001) than in clinically healthy dogs. Approximately at this level its indices in both groups were the same on the 3rd day after osteosynthesis, which is associated primarily with pain reaction in response to bone injury.
Conclusions 1. Application of Collapan for replacing bone defects in dogs with extracortical osteosynthesis is accompanied by a mechanical stop of bleeding, which leads to less expressed changes in the hemostatic system and endothelial function and reduces catabolic processes in the area of the fracture.
2. Replacement of bone defects by Collapan has no effect on the level of post-traumatic stress, but the presence in its composition alien for dogs collagen causes elongation stage of inflammatory resorption.
Prospects for further research is to establish clinical and biochemical criteria complicated course of reparative osteogenesis by using Collapan in dogs with comminuted fractures of tubular bones.
Key words: dog, bone fractures, glycosaminoglycans, glycoproteins, soluble fibrin, glucose, nitric oxide, Collapan.
1. Rublenko S.V. Monіtoring veterinarnoї dopomogi і struktura hіrurgіchnoї patologії sered drіbnih domashnіh tvarin v umovah mіs'koї klіnіki / S.V. Rublenko, O.V. Єroshenko // Vіsnik Sums'kogo NAU. – Sumi, 2012. – Vip. 1 (30). – S. 150-154.
2. Pustovіt R.V. Monіtoring hіrurgіchnoї patologії sered drіbnih svіjs'kih tvarin DLVM u Kiїvs'komu rajonі m. Odesi za 2003–2005 roki / R.V. Pustovіt, Ju.M. Danilejko, M.V. Rublenko // Vіsnik Bіlocerkіv. derzh. agrar. un–tu. – Bіla Cerkva. – 2006. – Vip. 36. – S. 132–137.
3. Petrenko O.F. Osoblivostі perelomіv kіstok kіncіvok u svіjs'kih tvarin / O.F. Petrenko // Veterinarna medicina Ukraїni. – 2002. – №5. – S. 16–17.
4. Teljatnіkov A.V. Poshirennja perelomіv kіstok u sobak / A.V. Teljatnіkov // Naukovij vіsnik veterinarnoї medicini: Zb. nauk. prac'. – Bіla Cerkva, 2013. – Vip. 11 (101). – S. 149 – 153.
5. Haaland P.J. Appendicular fracture repair in dogs using the locking compression plate system: 47 cases / P.J. Haaland, L. Sjöström; M. Devor; et ol // Vet. Comp. Orthop Traumatol. – 2009. Vol. 4. – R. 309–315.
6. Semenjak S.A. Struktura perelomіv kіstok u sobak v umovah megapolіsu / S.A. Semenjak, S.V. Rublenko, Ju.M. Danilejko // Vіsnik Bіlocerkіv. derzh. agrar. un–tu. – Bіla Cerkva. – 2014. – Vip. 13 (108). – S. 218–223.
7. Sahno N. V. Ocenka sposobov fiksacii otlomkov trubchatyh kostej pri kosyh perelomah // Aktual'nye problemy diagnostiki, terapii i profilaktiki boleznej domashnih zhivotnyh – Mat–ly mezhd. nauch.-prakt. konf., posvjaw. 80-letiju fakul'teta veterinarnoj mediciny FGOU VPO «Voronezhskij gosudarstvennyj agrarnyj universitet im. K.D. Glinki», 21–22 sentjabrja 2006. – Voronezh, 2006. – S. 255–258.
8. Stimuljacіja reparativnogo osteogenezu u tvarin vіtamіnami ta mіkroelementami / O. F. Petrenko, V. P. Suhonos, V. B. Borisevich [ta іn.] // Metodichnі rekomendacії, zatverdzh. naukovo-metodichnoju komіsієju Derzhavnogo departamentu vetmedicini MAP Ukraїni. – 20.12.2006 r. – Kiїv, 2007. – 28 s.
9. Smurna O.V. Zastosuvannja ekstrakortikal'nogo osteosintezu ta gіdroksilapatitu "kergap" pri perelomah klubovoї kіstki u sobak: avtoref. dis. na zdobuttja nauk. stupenja kand. vet. nauk: spec. 16.00.05 „Veterinarna hіrurgіja”/ O.V. Smurna – Bіla Cerkva, 2009. – 20 s.
10. Berdjugina O.V. Immunologicheskij monitoring zamedlennogo kosteobrazovanija / O.V. Berdjugina, K.A. Berdjugin // Citokiny i vospalenie. – 2010. – T.9, №1. – S. 66–70.
11. Rublenko M.V. Zastosuvannja traneksamovoї kisloti і acelіzinu za osteosintezu perelomіv trubchastih kіstok u sobak / M.V. Rublenko, O.V. Єroshenko, V.M. Vlasenko // Veterinarna bіotehnologіja. – 2013. – №22. – S. 496–505
12. Rublenko M.V. Patogenetichna rol' oksidu azotu v umovah zapal'no-reparativnogo procesu pri perelomah trubchastih kіstok u sobak ta jogo korekcіja Іmunom-depo / M.V. Rublenko, V.S. Shaganenko // Bіologіja tvarin. – 2011. – T. 13, №1–2. – S. 340–346.
13. Andrієc' V.G. Klіnіko-rentgenologіchna harakteristika ta citokіnova reguljacіja reparativnogo osteogenezu u vipadku іntrameduljarnogo osteosintezu kіstok u sobak / V.G. Andrієc' // Naukovij vіsnik L'vіvs'kogo nacіonal'nogo unіversitetu veterinarnoї medicini ta bіotehnologії іm. S.G. Gzhic'kogo. – 2014. –T. 16, № 3 (60), Ch. 1. – S. 27–37.
14. Rublenko M.V. Markeri metabolіzmu spoluchnoї tkanini za perelomіv trubchastih kіstok u sobak / M.V. Rublenko, O.V. Єroshenko // Veterinarna medicina: Mіzhvіd. temat. nauk. zb. – Harkіv, 2012. – Vip. 96. – S. 321–324.
15. Pustovіt R.V. Gemostaz ta jogo korekcіja pri perelomah trubchastih kіstok u sobak: avtoref. dis. na zdobuttja nauk. stupenja kand. vet. nauk : spec. 16.00.05 “Veterinarna hіrurgіja” / R.V. Pustovіt. – Bіla Cerkva, 2008. – 22 s.
16. Paskalev M. Changes in some serum bone markers after Experimental fracture and intramedullary Osteosynthesis in dogs / M. Paskalev, s. Krastev, j. Filipov // Trakia journal of sciences. – 2005. Vol. 3. – R. 46-50.
17. Sousa C. Serum total and bone alkaline phosphatase and tartrate-resistant acid phosphatase activities for the assessment of bone fracture healing in dogs / C. Sousa, H. Abreu, C. Viegas // Arq. Bras. Med. Vet. Zootec. – 2011. – Vol. 63. – R.1007-1011.
18. Theyse, L. The efficacy of the bone markers osteocalcin and the carboxyterminal cross-linked telopeptide of type–I collagen in evaluating osteogenesis in a canine crural lengthening model / Theyse, L.; Mol, J.A.; Voorhout, G. et al // Vet. J. – 2005.– Vol.171. – R.525-531.
19. Berchenko G. N., Kesjan G. A. Aktivizacija reparativnogo osteogeneza pri zapolnenii segmentarnogo defekta dlinnoj trubchatoj kosti kompozicionnym preparatom Kollapan // Travma. – 2008. – T. 9. № 3. – S. 282–286.
20. Petrenko O. F. Racіonal'nі metodi osteosintezu ta stimuljacіja reparativnogo osteogenezu u tvarin: avtoref. dis. na zdobuttja nauk. stupenja d-ra. vet. nauk : spec. 16.00.05 Veterinarna hіrurgіja / O. F. Petrenko – Bіla Cerkva, 2002. – 34 s.
21. Rublenko S.V. Zastosuvannja mіscevih anestetikіv u kompleksnomu zneboljuvannі za abdomіnal'nih operativnih vtruchan' u sobak / S.V. Rublenko, A.V. Mel'nіkov, A.V. Berezovs'kij // Veterinarna bіotehnologіja. – Nіzhin, 2013. – Vip 22. – S. 505–511.
22. Neverov N.V. Frakcionnoe opredelenie soderzhanija geksoz, svjazannyh s belkami, v syvorotke krovi. / N.V. Neverov, N.I. Titorenko // Lab. delo. – 1979. – № 6. – S. 323–325.
23. Opredelenie rastvorimogo fibrina v plazme krovi / T. V. Vareckaja, L. I. Mihajlovskaja, L. A. Svital'skaja [i dr.] // Klin. lab. diagnostika. – 1992. – №7–8. – S. 10–14.
24. Sposob opredelenija "srednih molekul" / V.V. Nikolajchik, V.M. Moin, V.V. Kirkovskij // Lab. delo. – 1991. – №10. – S. 13–18.
25. Golikov P.P. Oksid azota v klinike neotlozhnyh zabolevanij. / P.P. Golikov ‒ M: ID «Medpraktika» – M, 2004. ‒ 180 s.