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Hematological indexes of dogs at dirofilariosis
Laboratory diagnostics for any animal pathology refers to special research methods and helps not only in establishing the final diagnosis, but also in expanding data on the course of primary pathology and determining secondary complications based on changes in blood parameters. For this purpose, a clinical study of the blood of dogs with varying degrees of intensity of dirofilariasis invasion was carried out.
It was found that the number of erythrocytes decreased in animals of the experimental groups, depending on the intensity of invasion. Severe erythropenia was found in the group of animals with the highest degree of invasion intensity
(2.04±0.50 1012/l (p<0.001). It increased with an increasing in the degree of invasion and ESR up to 40.28±12.67 mm/h (p<0.05) and 62.50±14.16 mm/h (p<0.01). Thrombocyto-penia at 199.13±55.31 109/l was found in dogs in the group with the highest degree of invasion. Oligochromemia in animals also depended on the intensity of invasion and was
94.44±12.30 g/l (p<0.05) and 52.00±12.69 g/l (p<0.001). In dogs with the highest number of dirofilaria larvae, the hematocrit was 19.75±6.34 % (p<0.01) and leukocytosis developed (20.53±8.91 109/l). By calculating the leukocyte count in dogs with the highest degree of dirofilariasis invasion, a significant increase in the number of segmented neu-trophils was found to 86.00±10.43 % (p<0.1), eosinophils up to 27.88±5.54 % (p<0.001), basophils up to 1.75±1.39 % (p<0.001), appeared in blood of sick animals young neutro-phils (0.50±1.07 %, p<0.001).
The established changes in hematological parameters are associated with the development of hemolytic anemia during invasion and the presence of latent bleeding associated with the destruction of the vascular wall by dirofilariae. The relative leukocytosis, which were diagnosed in sick dogs, is associated with the presence of nematodes and the development of inflammatory processes at the sites of localization and migration of dirofilaria. It is possible that leukocytosis and a shift of the leukocyte formula to the left are associated with intoxication of the body.
Key words: dogs, heartworm disease, Dirofilaria im-mitis, blood, erythrocytes, leukocytes, leukocyte formula, hemoglobin, hematocrit, erythrocyte sedimentation rate.
https://orcid.org/0000-0002-6984-0185REFERENCES
1. Hou, H., Guoshun, S., Wei, W. (2011). Prevalence of Dirofilaria immitis infection in dogs from Dandong, China. Veterinary Parasitology. no. 183, pp. 189–193. Available at:https://doi.org/10.1016/j.vetpar.2011.06.016
2. Anvari, D., Saadati, D., Siyadatpanah, A. (2019). Prevalence of dirofilariasis in shepherd and stray dogs in Iranshahr, southeast of Iran. Journal of Parasitic Diseases. no. 43, pp. 319–323. Available at:https://doi.org/10.1007/ s12639-019-01096-5
3. Jevstaf’jeva, V.O., Sorokova, S.S., Sorokova, V.V. (2015). Osoblyvosti gistomorfologichnoi' diagnostyky dyrofiljariozu sobak [Features of histomorphological diagnosis of dirofilariosis in dogs]. Suchasni tendencii' provedennja laboratornyh doslidzhen' u veterynarnij medycyni: mat-ly Vseukr. nauk. seminaru, prysv. 20-richchju zasnuvannja kaf-ry parazytologii' ta veterynarno-sanitarnoi' ekspertyzy PDAA [Current trends in laboratory research in veterinary medicine: Mat-ly All-Ukrainian. Science. seminar, dedicated to 20th anniversary of the founding of the Department of Parasitology and Veterinary Sanitary Examination PDAA]. Poltava: LLC SPE "Ukrpromtorgservice", 45 p.
4. Luckevych, K.O., Galat, M.V. (2012). Patologoanatomichni zminy v organizmi sobaky, sprychyneni parazytuvannjam gel'mintiv rodu Dirofilaria [Pathological changes in the body of dogs caused by parasitism of helminths of the genus Dirofilaria]. Naukovyj visnyk L'vivs'kogo nacional'nogo universytetu veterynarnoi' medycyny ta biotehnologij im. G'zhyc'kogo [Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv] no. 14, no. 2(1), pp. 208–211.
5. Stepanenko, O.Ju., Solovjova, L.M. (2018). Diagnostyka ta likuvannja sobak za dyrofiljariozu [Diagnosis and treatment of dogs at dirofilariosis]. Aktual'ni problemy veterynarnoi' medycyny: mat-ly mizhnar. nauk.-prakt. konf-i'. magistrantiv, m. Bila Cerkva, 22 lystopada 2018 r. [Actual problems of veterinary medicine: mat-li international. scientific-practical conf. undergraduates, Bila Tserkva, November 22, 2018]. Bila Tserkva, 59 p.
6. Choi, B.S., Moon, H., Suh, S.I., Hyun, C. (2017). Evaluation of serum symmetric dimethylarginine in dogs with heartworm infection. Can J Vet Res. no. 81(3), pp. 228–230.
7. Solovjova, L.M. (2019). Laboratorna diagnostyka ta likuvannja sobak za dyrofiljariozu [Laboratory diagnosis and treatment of dogs at dirofilariosis]. Vyrishennja suchasnyh problem u veterynarnij medycyni: mat-ly V Vseukrai'ns'koi' naukovo-praktychnoi' Internet-konferencii', 14–15 ljutogo 2019 roku [Solving modern problems in veterinary medicine: materials of the V All-Ukrainian scientific-practical Internet conference, February 14-15, 2019]. Poltava: LLC SPE "Ukrpromtorgservice", 170 p.
8. Jastreb, V.B. (2009). Klynycheskye pryznaky dyrofyljaryoza sobak, vuzvannogo Dirofilaria immitis y D. repens [Clinical signs of canine dirofilariasis caused by Dirofilaria immitis and D. repens]. Rossyjskyj parazytologycheskyj zhurnal [Russian Journal of Parasitology]. no. 2, pp. 86–91.
9. Mejia, J.S., Carlow, C.K. (1994). An analysis of the humoral immune response of dogs following vaccination with irradiated infective larvae of Dirofilaria immitis. Parasite Immunol. no. 16(3), pp. 157–164. Available at:https://doi. org/10.1111/j.1365-3024.1994.tb00335.x
10. Beskrovnaja, Ju.G. Dyrofyljaryoz na juge Rossyy (rasprostranenye y dyagnostyka): avtoref. … kand. byol. nayk: 03.00.19. [Dirofilariasis in the south of Russia (distribution and diagnostics): author's abstract dissertation of the candidate of biological sciences: 03.00.19.]. Moscow, 26 p.
11. Jastreb, V.B. (2011). Pryzhyznennaja dyagnostyka dyrofyljaryoza [Intravital diagnosis of dirofilariasis]. Teoryja y praktyka parazytarnyh boleznej zhyvotnyh [Theory and practice of parasitic diseases of animals]. no. 12, pp. 587–591.
12. Ludders, J.W., Grauer, G.F., Dubielzig, R.R. (1988). Renal microcirculatory and correlated histologic changes associated with dirofilariasis in dogs. American Journal of Veterinary Research. no. 49(6), pp. 826–830.
13. Chystenko, G.N., Veden'kov, A.L., Dronyna, A.M., Semyzhon, O.A. (2013). Dyrofyljaryoz cheloveka [Human dirofilariasis]. Medycynskyj zhurnal [Medical journal]. no. 3(45), pp. 30–33.
14. Ermakov, A.M., Bojko, V.P., Kolodyj, Y.V. (2012). Gemodynamyka pochek y korrekcyja ee narushenyj pry dyrofyljaryoze u sobak [Renal hemodynamics and correction of its disorders in dirofilariasis in dogs]. Veterynaryja Kubany [Veterinary medicine of the Kuban]. no. 2, pp. 5–6.
15. Кitagawa, H., Sasaki, Y., Ishihara, K. (1986). Clinical Studies on Canine Dirofilarial Hemoglobinuria: Relationship between the Presence of Heartworm Mass at the Tricuspid Valve Orifice and Plasma Hemoglobin Concentration. The Japanese journal of veterinary science. no. 48(1), pp. 99–103. Available at:https://doi.org/10.1292/jvms1939.48.99
16. Soloviova, L. (2017). Distribution and treatment of Dirofilariosis of dogs in the town of Bila Tserkva. Naukovyj visnyk veterynarnoi' medycyny [Scientific Bulletin of Veterinary Medicine]. no. 2, pp. 127–131.
17. Sharma, M.C., Pachauri, S.P. (1982). Blood cellular and biochemical studies in canine dirofilariasis. Vet. Res. Commun. no. 5, 3, pp. 295–300. Available at:https://doi. org/10.1007/BF02214997
18. Kaewthamarson, M. (2008). Microfilaruria caused by canine dirofilariasis (Dirofilaria immitis): an unusual clinical presence. Comparative Clinical Pathology. pp. 61–65. Available at:https://doi.org/10.1007/s00580-007-0675-1
19. Roslyj, Y.M., Vodolazhskaja, M.G. (2009). Sravnytel'nye podhodu v ocenke sostojanyja cheloveka y zhyvotnyh: Byohymycheskye osobennosty eksperymental'no-klynycheskogo sostojanyja zhyvotnyh [Comparative approaches in assessing the state of humans and animals: Biochemical characteristics of the experimental and clinical state of animals]. Vestnyk veterynaryy [Veterinary bulletin]. Issue 50, no. 3, pp. 63–72.
20. Atwell, R.B., Buoro, I.B. (1983). Clinical presentations of canine dirofilariasis with relation to their haematological and microfilarial status. Res. Vet. Sci. no. 35, pp. 364–366. Available at:https://doi.org/10.1016/S0034-5288(18)32034-4
21. Harvey, J.W. (1982). Myeloproliferative disease with megakaryocytic predominance in a dog with occult dirofilariasis. Vet. Clin. Pathol. no. 11, pp. 5–11. Available at:https://doi.org/10.1111/j.1939-165X.1982.tb00811.x
22. Paramonov, V.V. (2014). Patomorfologyja, patogenez, dyagnostyka y lechenye dyrofyljaryoza sobak: avtoref. … kand. vet. nayk: 06.02.01. [Pathomorphology, pathogenesis, diagnosis and treatment of dirofilariasis of dogs: abstract of the dissertation of the candidate of veterinary sciences: 06.02.01.]. Ufa, 24 p.
23. Segedy, A.K., Hayden, D.W. (1978). Cerebral vascular accident caused by Dirofilaria immitis in a dog. J. Am. An. Hosp. Assoc. no. 14, pp. 752–756.
24. Kochetkova, A.Ju. (2016). Byohymycheskye aspekty patogeneza pry dyrofyljaryoze sobak, vyzvannogo Dirofilaria immitis: dys. kand. byol. nauk [Biochemical aspects of pathogenesis at dirofilariasis of dogs caused by Dirofilaria immitis: dissertation of the candidate of biological sciences: 06.02.01.]. Stavropol, 132 p.
25. Kamyshnykov, V.S. (2004). Spravochnyk po klynyko-byohymycheskym yssledovanyjam y laboratornoj dyagnostyke [Handbook on clinical and biochemical research and laboratory diagnostics]. Moscow: MEDpress-inform, 920 p.
26. Levchenko, V.I., Golovaha, V.I., Kondrahin, I.P. (2010). Metody laboratornoi' klinichnoi' diagnostyky hvorob tvaryn: navchal'ne vydannja [Methods of laboratory clinical diagnosis of animal diseases: educational publication]. Kyiv: Agricultural education, 437 p.
27. Jastreb V.B. (2009). Klinicheskie priznaki dirofiljarioza sobak, vyzvannogo Dirofilaria immitis i D. repens [Clinical signs of canine dirofilariasis caused by Dirofilaria immitis and D. repens]. Rossijskij parazitologicheskij zhurnal [Russian parasitological journal]. no 2, pp. 86–91.
28. Vasylyk, N.S. (2004). Morfofunkcional'ni zminy ta adaptacijno-kompensatorni reakcii' v organizmi sobak za dyrofiljariozu: avtoref. dys. … kand. vet. nauk: 16.00.02. [Morphofunctional changes and adaptive-compensatory reactions in the body of dogs with heartworm disease: abstract of the dissertation of the candidate of veterinary sciences:16.00.02.]. Kyiv, 26 p.
29. Solovjova, L.M., Artemenko, L.P., Antipov, A.A., Bahur, T.I. (2018). Dyrofiljarioz: navch. posibnyk [Heartworm disease: a textbook]. Bila Tserkva, 15 p.
30. Dahno, I.Ju. (2013). Dyrofiljarioz sobak (poshyrennja, diagnostyka ta zahody borot'by): avtoref. dys. … kand. vet. nauk: 16.00.11. [Heartworm disease of dogs (spread, diagnosis and control measures): abstract of the dissertation of the candidate of veterinary sciences: 16.00.11.]. Kyiv, 21 p.
31. Tarasenko, A.V., Alekseev, S.A., Fedorov, V.N., Dudko, A.A. (2016). Ocenka pokazatelej jendogennoj intoksikacii i prognozirovanija neblagoprijatnyh ishodov u pacientov s razlichnymi formami nekrotizirujushhego pankreatita [Assessment of indicators of endogenous intoxication and prediction of unfavorable outcomes in patients with various forms of necrotizing pancreatitis]. Voennaja medicina [Military medicine]. no 2, pp. 59–62.
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