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Species composition and antibiotic resistance of skin flora of healthy and patients with pyoderma dogs

To study the microflora of the skin were selected 10 healthy and 27 patients with pyoderma dogs of various breeds aged 1 to 10 years.

When sowing leather healthy dogs identified 12 strains represented in 7 cases (58.3 %), saprophytic (Staphylococcus epidermidis), and opportunistic microorganisms: Staphylococcus aureus – 3 cases (25 %) and 1 case ( for 8,3 %) Escherichia coli and Klebsiella pneumoniae. In 3 cases showed the association of microorganisms.

We determined the sensitivity of microorganisms to selected major groups of antibiotics used in veterinary medicine today, namely: amoxicillin, gentamicin, doxycycline, zinaprim, kobaktan, clarithromycin, ofloxacin, penbeks, spyektovet, farmazyn, cefalexin, ceftriaxon, cefotaxime and enrofloxacin.

Data on antibiotic susceptibility of strains of certain species of microorganisms indicate that Staphylococcus epidermidis in 100 % of cases had a high sensitivity to antibiotics; Staphylococcus aureus in 76.1 % of cases was high, 21.4% moderate and 2.4 % low sensitivity to antibiotics.

Escherichia coli was highly sensitive to antibiotics in 64.3 % of cases and moderate sensitivity to them in 35.7 % of cases; Klebsiella pneumonia had a high sensitivity to antibiotics

That is, all strains saprophyte microflora of the skin of healthy dogs (Staphylococcus epidermidis) were highly sensitive to antibiotics. Among opportunistic pathogens (Staphylococcus aureus, Escherichia coli and Klebsiella pneumonia) a significant portion strains had moderate or even low degree of sensitivity to antibiotics. It is a testament to their steadiness and ability in certain circumstances to cause or exacerbate infection.

When sowing leather patients with pyoderma dogs in 18 ( 66.6 %) cases were identified monoculture microorganisms in 9 ( 33.3 %) - their association. Most commonly isolated Staphylococcus aureus (42,1 % of cases), often Klebsiella pneumonia ( 21 % of cases) and Escherichia coli (13,1 % of cases). Percentage detection of other microorganisms (Candida albicans, Enterobacter aerogenes, Pseudomonas aeruginosa) was insignificant.

Preferential detection of Staphylococcus in patients with purulent exudate dogs indicates their important role in the development of pyoderma.

Microorganisms isolated from skin with pyoderma had significantly lower sensitivity to antibiotics than those matched were isolated from the skin of healthy dogs. This indicates an increasing there resistance to drugs in the development of inflammation in the skin

Thus, in strains Staphylococcus aureus, isolated from the skin of healthy dogs, 76.1 % had a high sensitivity to antibiotics, while only 34.8 % of pyoderma that half. Instead, the number of strains with moderate and low sensitivity to antibiotics in pyoderma increased significantly - in accordance with 21.4 % to 43.75 % and from 2.4 % to 14.7 % and 6.7 % of strains of Staphylococcus aureus was complete lack of sensitivity to it .

In strains of Escherichia coli, isolated from the skin of healthy dogs, 64.3 % had a high sensitivity to antibiotics, while pyoderma - only 40 %. However, in 15.7 % of strains appeared low sensitivity to antibiotics, and in 17.1 % it was absent.

All strains of Klebsiella pneumoniae, isolated from the skin of healthy dogs were high ( 42.8 %) or moderate ( 57.2 %), sensitivity to antibiotics, while these figures pyoderma fell under - 12.5 % and 15.2% . However, in 34.8 % of strains appeared low sensitivity to antibiotics, and in 37.5 % it was absent.

Key words: microflora of the skin of dogs, pyoderma, sensitivity of microflora to antibiotics.

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