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Dynamics of some indicators of natural resistance and antioxidant defense system during the application of polcarbonate inactivated vaccine "PARCELS PARCEL DILUVAC FORTE" (PORCILIS PORCOLI DILUVAC FORTE)

Among many animal diseases - infectious - cause the most serious economic losses, and the disease of newborn animals with symptoms of disorder of the gastrointestinal tract, remains one of the thorniest problems of industrial livestock around the world. Prevention of animal diseases caused by pathogenic effect esherihy primarily must be aimed at forming a viable offspring in utero and increased resistance in newborn animals, so it is necessary to use scientific-based complex preventative measures, including vaccination that takes a leading position.

Analysis of recent research and publications. Domestic scientists proposed associated vaccine "Pasak" for the prevention of certain factorial diseases including colibacillosis. Production research confirmed the effectiveness of preventive expressed subunit vaccine against colibacillosis based on pathogenicity factors of the pathogen, and the possibility and feasibility of its use for specific prevention of infection in young animals of different species. However, the application of vaccines in addition to positive action (acquired immunity) may be negative effects in a variety of side effects, in particular, the emergence imunosupresornogic syndrome and increased intensity of lipid peroxidation, which may result in destructive processes at the cellular level, what we decided to concentrate its attention.

The objective and the tasks of the research – studying of the dynamics of changes of natural resistance and antioxidant defense system in the application of inactivated vaccines "Portsylis Porkoli DB» (PORCILIS PORCOLI DILUVAC FORTE) on the body of pigs.

The object of research – sows  of two-, three years old of Large White breed. They were vaccinated with "Portsilis Porkoli» DF against colibacillosis intramuscularly in the neck at a dose of 2 ml / head for (8-12) weeks before farrowing. It is widely immunization scheme, which includes two, triple vaccination to pregnant   animals against esherihioz, the latter introducing immunogenic produced by 14-21 days before the anticipated birth. This arrangement provides a vaccination maximum accumulation of immunoglobulins (specific antibodies) in colostrums of animals and their transmission to the newborn offspring.

Two groups of animals were formed to conduct our experiment: control and experimental (n = 10). Control animals were injected intramuscularly  2 ml of 0.85% sodium chloride solution. Blood samples were taken in the morning three times: before vaccination, 14 and 28 days after inoculation. Vaccinated and control animals were kept in pre-peeled, washed and disinfected areas. Daily clinical examination was performed with thermometry.

Serum was obtained by conventional sludge. Tubes with the blood were placed in an incubator at 37 ± 1 ° C for 2-3 hours and after defending the serum tubes were placed in the refrigerator at a temperature of 4-8 ° C for 1-2 h. Transparent serum aseptically poured into sterile tubes and kept at a temperature of minus 18,0 ± 0,5 ° C.

During the experiment, the changes in the clinical status of the sows as the experimental and control groups were observed. It is established that in animals of both groups during the experiment, the clinical condition was satisfactory, the body temperature averaged 38,9 ± 0,06 and 39,3 ± 0,09ºC, respiratory rate - 17,0 ± 1,4 and     16 ± 1 6  breathing movements / min.

Summarizing the results of the research, it should be noted that the drug is not toxic and has good immunogenic properties. Proof of this is increasing the amount of globulin by 9.2% in the 28-day of study compared to the beginning of the experiment and 20.7% of the control group of pigs (P <0.05); lizotsymic activity, respectively, 6.8 and 11.2%; and circulating immune complexes in the blood serum of sows  from experimental group. Especially note the number of CIC, the level of which the 14-day  of study increased by 20.0% compared with the original data, which is evidence of activation of complement and B lymphocytes.

The absence of a toxic effect of the drug is confirmed by the relatively constant value of the index AOC experimental animals that were close in value to the values of the control group of sows on the 28th day of the study. After the third selection in the blood of animals in the control group level of seromucoid increased by 50% from baseline when experimental sows it was close to baseline values throughout the study period, confirming the positive effect of the drug on the body of experimental animals. Obviously, these positive indicators are also associated with an aqueous solution of α-tocopherol acetate, which is part of the vaccine and helps to reactivate the enzymes of the antioxidant system and reduction of the intermediate products of lipid peroxidation. We believe that the regulatory documentation does not include changes in lipid peroxidation - AOC that are very informative.

Thus, the results of our own studies confirm the efficacy and safety of this vaccine under the standard documentation (industry standard).

Key words: total protein, globulins, CEC, seromucoid, immune reactivity, the safety of vaccines.

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