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Features of clinical and pathomorphological picture in spontaneous infection of a domestic cat (lat. Félis cátus) with SARS-CoV-2 coronavirus

Today, cases of infection of various animal species with the new SARS-CoV-2 coronavirus have become increasingly common. This virus has been isolated from numerous representatives of the feline family, European mink, ferret, raccoon dog, domestic dog, a number of primates and a number of other animals. In most susceptible animals, infection with this virus is manifested by certain clinical symptoms, the intensity of which depends on the type and age of the animals, on the presence of concomitant diseases. The pathological process can end in death with the development of characteristic pathoanatomical and histological changes. According to the results of preliminary studies conducted in the Republic of Belarus, the circulation of SARS-CoV-2 was detected in domestic cats. All the animals had a history of contact with COVID-19-infected owners. The aim of this work was to determine the features of clinical and pathoanatomical manifestations, histological changes in domestic cats infected with SARS-CoV-2. The studies were conducted among various sex and age groups of domestic cats. In total, we conducted a study of 300 flushes from various sexes and ages and pedigreed, autopsy of 10 corpses. The work was carried out in the Vitebsk State Academy of Veterinary Medicine, the Vitebsk Regional Veterinary Laboratory, the RSPC "Epidemiology and Microbiology" in Minsk, in the Belarusian State Veterinary Center. The circulation of SARS-CoV-2 in animals was determined by polymerase chain reaction (RT - PCR). When dissecting the corpses of animals, the nature and severity of pathomorphological changes were taken into account, a pathoanatomical diagnosis was made, and macrophotography was performed in natural light. The main clinical symptoms of the disease in adult animals are depression, refusal to feed, cough, shortness of breath; in young animals, rhinitis, conjunctivitis, diarrhea are often observed. When dissecting dead animals, macro-changes and micro-changes in organs and tissues are noted, indicating the development of pathological processes, both in the acute course of the disease and in the chronic one. Thus, the conducted studies and the results obtained confirmed and supplemented the data of world researchers, made it possible to determine the leading clinical symptoms of the disease and pathoanatomical changes in the domestic cat when infected with SARS-CoV-2. The obtained data of histological changes allowed a deeper and more detailed assessment and study of the pathogenesis of the disease, which will contribute to a rational approach in choosing the means of therapy of this disease.

Key words: cats, coronavirus, SARS-CoV-2, clinical symptoms, pathoanatomical changes, histological examination.

 

  1. Nikiforov, V. (2020). Novaya koronavirusnaya infekciya (COVID-19): e`tiologiya, e`pidemiologiya, klinika, diagnostika, lechenie i profilaktika [New coronavirus infection (COVID-19): etiology, epidemiology, clinic, diagnosis, treatment and prevention]. Moscow, 48 p. Doi:10.20514/2226- 6704-2020-10-2-87-93
  2. Saksena, Shajlendra K. (2020). Koronavirusnaya bolezn` 2019 (COVID-19) [Coronavirus disease 2019 (COVID-19)]. Singapur: Springer, 213 p. Doi:10.1007/978- 981-15-4814-7
  3. WHO Coronavirus Disease (COVID-19) Dashboard. Available at: https://covid19.who.int/table (accessed on 20 January 2021)
  4. Ahn, D.G. (2020). Current status of epidemiology, diagnosis, therapeutics, and vaccines for novel coronavirus disease 2019 (COVID-19). J Microbiol Biotechnol. Vol. 30(3), pp. 313–324. Doi:10.4014/jmb.2003.03011.
  5. OIE Technical Factsheet on Infection with SARSCoV-2 in Animals. Available at: http://oie.int/fileadmin/ Home/eng/Our_scientific_expertise/docs/pdf/COV-19/A_ Factsheet_SARS-CoV-2.pdf
  6. OIE Guidance on working with farmed animals of species susceptible to infection with SARS-CoV-2 Available at: http://oie.int/fileadmin/Home/MM/Draft_OIE_Guidance_ farmed_animals_cleanMS05.11.pdf
  7. World Organisation for Animal Health (OIE). (2021). OIE Technical Factsheet: Infection with SARS-CoV-2 in animals. Available at: http://rr-asia.oie.int/wp-content/uploads/2020/06/200608_a_factsheet_sar... (accessed on 20 January 2021)
  8. World Organisation for Animal Health (OIE). (2021). OIE COVID-19 Portal: Events in animals. Available at: http:// oie.int/en/scientific-expertise/specific-information-and-recommendations/questions-and-answers-on-2019novel-coronavirus/events-in-animals/. (accessed on 20 January 2021)
  9. World Organisation for Animal Health (OIE). (2020). Considerations for sampling, testing, and reporting of SARS-CoV-2 in animals. Available at: http://oie.int/fileadmin/Home/MM/A_Sampling_Testing_and_Reporting_of_ SARS-CoV-2_in_animals_3_July_2020.pdf (accessed on 20 January 2021)
  10. FAO, 2021. COVID-19 and animals. Information of risk mitigation measures for livestock and agricultural professionals. Available at: http://fao.org/documents/card/en/c/ cb2549en. (accessed on 20 January 2021)
  11. FAO, Exposure of humans or animals to SARSCoV-2 from wild, livestock, companion and aquatic animals. Available at: http://fao.org/3/ca9959en/CA9959EN.pdf (accessed on 20 January 2021)
  12. WHO, Origins of the SARS-CoV-2 virus. Available at: http://who.int/health-topics/coronavirus/who-recommendations-to-reduce-r....(accessed on 20 January 2021)
  13. Centres for Disease Control COVID-19 and Animals. Available at: http://cdc.gov/coronavirus/2019-ncov/ daily-life-coping/animals.html
  14. Mahdy, M.A.A., Younis, W., Ewaida, Z. (2020). An Overview of SARS-CoV-2 and Animal Infection. Front. Vet. Sci. Vol. 7, 1084 p. Available at: http://frontiersin.org/articles/10.3389/fvets.2020.596391/full (accessed on 20 January 2021)
  15. Hobbs, E.C., Reid, T.J. (2020). Animals and SARSCoV-2: Species susceptibility and viral transmission in experimental and natural conditions, and the potential implications for community transmission. Trans. Emerg. Dis, Online ahead of print. Available at: http://onlinelibrary.wiley.com/ Doi/10.1111/tbed.13885 (accessed on 20 January 2021)
  16. Kim, Y.I, Kim, S.G., Kim E.H., Park S.J., Yu K.M., Chang J.H. (2020). Infection and Rapid Transmission of SARS-CoV-2 in Ferrets. Cell Host Microbe. Vol. 27 (5), pp. 704–709. Available at: http://sciencedirect.com/science/ article/pii/S1931312820301876 (accessed on 20 January 2021)
  17. European Centre for Disease Prevention and Control (ECDPC). (2020). Detection of new SARS-CoV-2 variants related to mink. Available at: http://ecdc.europa.eu/sites/ default/files/documents/RRASARS-CoV-2-in-mink-12- nov-2020.pdf (accessed on 20 January 2021)
  18. World Organisation for Animal Health (OIE). (2021). OIE statement on COVID-19 and mink. Available at: http://oie.int/en/for-the-media/press-releases/detail/article/ oie-statement-on-covid-19-and-mink/. (accessed on 20 January 2021)
  19. WHO, Disease Outbreak News, SARS-CoV-2 mink-associated variant strain – Denmark. Available at: http://who.int/csr/don/03-december-2020-mink-associated-sars-cov2-denmar... (accessed on 20 January 2021)
  20. United States Department of Agriculture Response and containment guidelines: Interim Guidance for Animal Health and Public Health Officials Managing Farmed Mink and other Farmed Mustelids with SARS-CoV-2. Available at: http://aphis.usda.gov/publications/animal_health/sarscov-2-mink-guidance.pdf
  21. United States Department of Agriculture (USDA). (2020). Response & Containment Guidelines Interim Guidance for Animal Health and Public Health Officials Managing Farmed Mink and other Farmed Mustelids with SARS-CoV-2. Available at: http://aphis.usda.gov/publications/animal_health/ sars-cov-2-mink-guidance.pdf (accessed on 20 January 2021)
  22. United States Department of Agriculture (USDA). (2020). Mink (July 2020), USDA, National Agricultural Statistics Service. Available at: http://furcommission.com/ wp-content/uploads/2020/07/USDAmink2020.pdf (accessed on 20 January 2021)
  23. Guardian. (2020). Covid-19 mink variants discovered in humans in seven countries. Available at: http:// theguardian.com/environment/2020/nov/18/covid-19-minkvariants-discovered-in-humans-inseven-countries (accessed on 20 January 2021)
  24. United States Department of Agriculture (USDA). Interim SARS-CoV-2 Guidance and Recommendations for Farmed Mink and Other Mustelids. Available at: http://aphis. usda.gov/animal_health/one_health/downloads/sars-cov-2- guidance-for-farmed-mink.pdf (accessed on 20 January 2021)
  25. Centers for Disease Control and Prevention (2020). Steps to Prevent COVID-19 on Mink Farms. Available at: http://furcommission.com/wp-content/uploads/2020/11/ Mink-Training-Presentation_4Nov2020. pdf (accessed on 20 January 2021)
  26. DeLiberto, T., Shriner, S. (2020). Coronavirus disease 2019 update (536): Animal, USA (Utah), wild mink, first case. ProMED-mail, International Society for Infectious Diseases. Posted December. 13, Available at: http://sciencenewsforstudents.org/article/covid-19-coronavirus-minkutah-first-wild-animal-test-positive
  27. Oreshkova, N., Molenaar, R. J. (2020). SARSCoV-2 infection in farmed minks, the Netherlands, April 2020. BioRxiv. Doi: 10.1101/2020.05.18.101493.
  28. Oreshkova, N., Molenaar, R.J. (2020). SARA-CoV-2 infection in farmed minks, the Netherlands, April and May 2020. Eurosurv. Vol. 25 (23):2001005. Doi:10.2807/1560- 7917.ES.2020.25.23.2001005
  29. Dykstra, M. P., Baitchman, E. J. (2021). A Call for One Health in Medical Education: How the COVID-19 Pandemic Underscores the Need to Integrate Human, Animal, and Environmental Health. PMID: 33769340. AcadMed. Doi:10.1097/ACM.0000000000004072
  30. Smriti, Mallapaty. (2021). The search for animals harbouring coronavirus – and why it matters. Nature 591, pp. 26–28. Doi:10.1038/d41586-021-00531-z
  31. Son, H.D., Tu, C.C., Zhang, G.W., Wang, S.Y., Zheng, K., Lei, L.C., Chen, Q.X., Gao, Y.W., Zhou, H.Q., Xiang. (2005). Cross-host evolution of severe acute respiratory syndrome coronavirus in palm civet and human. Proc. Natl. Acad. Sci. USA. Vol.102, pp. 2430–2435. Doi:10.18632 / age.103988
  32. Shi, J., Wen, Z., Zhong, G., Yang, H., Wang, C., Huang, B., Liu, R., He, X., Shuai, L., Sun, Z. (2020). Susceptibility of ferrets, cats, dogs, and other domesticated animals to SARS-coronavirus 2. Science. Vol. 368, pp. 1016–1020. Doi:10.1126/science.abb7015
  33. Prudnikov, V.S., Belkin, B.L., German, S.P. (2021). Patomorfologicheskaya diagnostika boleznej sobak, koshek i pushny` h zverej [Pathomorphological diagnostics of diseases of dogs, cats and fur-bearing animals]. Vskry`tie i patomorfologicheskaya diagnostika boleznej zhivotny`h [Autopsy and pathomorphological diagnosis of animal diseases]. Vitebsk: VGAVM, pp. 168–192. (In Russian).
  34. Vy`soczki, A.E., Ly`senko, A. P., Baranovskaya, Z.N., Fomchenko, I.V., Ivanov, S.A., Rumachik, I.I. (2008). Metodicheskie ukazaniya po otboru patologicheskogo materiala, krovi, kormov i peresy`lki ih dlya laboratornogo issledovaniya [Guidelines for the selection of pathological material, blood, feed and sending them for laboratory testing]. Minsk, pp. 5–6. (In Russian). Available at: http://agriculture.uz/filesarchive/spravochnik_po_bakter.pdf
  35. Sarkisov, D. S., Petrova, Yu. L. (1996). Mikroskopicheskaya texnika: Rukovodstvo [Microscopic Technique: A Guide]. M. : Medicina. pp. 14–25, 36–50. (In Russian). Available at: http://studmed.ru/sarkisov-ds-perov-ds-mikroskopicheskaya-tehnika_b6c23ee3451.html
  36. Shuravin, P. V. (2020). Opisanie gistologicheskix preparatov: Rukovodstvo (Simple Pathology) [Description of histological preparations: A Guide (Simple Pathology)]. Moscow. pp. 5–14, 32–34, 64–70, 73–74, 77–82, 128, 135– 136, 139. (In Russian). Available at: http://simplepathology. ru/product/opisanie-gistologicheskih-preparatov-rukovodstvo-2020/
  37. Zajrat`yancz, O. V., Samsonova, M. V., Mixaleva, L. M., Chernyaev, A. L., Mishnev, O. D., Krupnov, N. M., Kalinin D. V. (2020). Patologicheskaya anatomiya COVID-19 : Atlas [Pathological anatomy of COVID-19: Atlas]. Moscow, GBU «NIIOZMM DZM», 142 p. (In Russian). Available at: http:// scsml.ru/info/Atlas.-COVID-19/HTML/index.html
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