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The influence of gender on blood biochemical parameters in sport horses
The article presents the results of research in the biochemical profile of blood in sport horses by gender (mares, stallions, geldings).
The study was conducted on two groups of healthy sport horses: Ukrainian warmblood and Hanoverian and Westphalian breeds. Animals in each group were divided by gender: mares, stallions, geldings.
Biochemical parameters of blood (total protein, albumin, total bilirubin, glucose, urea, creatinine, activity of AST, ALT, ALP and GGT investigated in 112 horses (38 mares, 34 stallions and 40 geldings).
The increasing use of horses in various activities associated with physical activity is a reflection of the physiological characteristics of animals, physiological state, feeding and effective training program. Biochemical blood test used to assess the functional state of internal organs, including the heart, liver, kidney, muscle function and their possible pathological changes.
To improve the efficiency of diagnosis of diseases should compare the results of research on reference values, taking into account age, breed and sex of the animals.
The aim of the researchers is to investigate the biochemical parameters of blood in sport horses of different reproductive status (mares, stallions, geldings).
The material for the study were 112 clinically healthy sport horses 4–15 years old Ukrainian warmblood horse (20 mares, 18 stallions, 22 gelding) and horses Hanoverian and Westphalian breed (18 mares, 16 stallions, 18 geldings), used in classical types of equestrian sport. The live weight of horses ranged from 350 to 600 kg. Horses were kept in conditions of equestrian sports institutions of western regions of Ukraine.
Collecting blood samples was carried out before the morning feeding. All the horses were resting at the time of the study and were clinically healthy.
For serum tubes were centrifuged at 3000 rev / min for 10 minutes. Serum of horses determined the contents of total protein, albumin, the concentration of total bilirubin, glucose, urea, creatinine, activity of aspartate aminotransferase (AST), alaninaminotrasferazy (ALT), alkaline phosphatase (ALP) and gamma glutamyl (HHTP) using automatic biochemical analyzer Mindray BS-120 (China) using reagents PZ Cormay SA (Poland).
Established in higher albumin content Ukrainian warmblood horse stallions compared to mares; the higher the concentration of total bilirubin in Ukrainian warmblood horses stallions compared to mares and geldings. Differences in glucose concentrations less pronounced, but in geldings of Ukrainian warmblood horses is higher than in stallions. Urea was higher in Ukrainian warmblood horses compared to the gelding. Creatinine concentration was higher in Ukrainian warmblood horses than geldings; geldings in Hanoverian and Westphalian breeds horses are higher compared to Ukrainian warmblood horses gelding.
Installed AST activity is higher in mares and geldings of Hanoverian and Westphalian horses compared to stallions; was also significant difference between gelding of Ukrainian warmblood and Hanoverian and Westphalian breeds horses. ALT activity was higher in geldings of Ukrainian warmblood horses compared to stallions; also significant difference in ALT activity established between stallions of Ukrainian warmblood and Hanoverian and Westphalian breeds horses. In mares of Ukrainian warmblood horses ALP activity was higher than that of stallions. Probably higher activity GGT set in Ukrainian warmblood horses mares and geldings compared to stallions; group of horses of Hanoverian and Westphalian breed has horses lower activity of GGT species found in horses compared to mares; in Ukrainian warmblood horses stallions GGT activity was significantly higher than that of counterparts of Hanoverian and Westphalian breeds horses.
Key words: horses, sex, biochemical profile, total protein, albumin, bilirubin, glucose, urea, creatinine, enzymes.
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