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Influence of microbiological starter of filler of biofilter of "Filtronorm D" on stored of rainbow trout
Creation in biofilters of favorable conditions for the existence of biocenoses secures RAS from the toxic effects of nitrites, which until recently did not attach great importance to toxicants for aquatic organisms. However, it has been found that they are very toxic to fish and aquatic invertebrates. The purpose of the work was to investigate the effect of microbiological starter of the "Filtronorm-D" biofilter filler on the behavior of adult rainbow trout during the launch of the biofilter.
Investigation of the influence of the microbial starter of the filtrotorm-D biofilter filler on the behavior and clinical condition and the preservation of rainbow trout during the launch of the biofilter of the ultrasound has been studied.
Evaluated the clinical signs of adult rainbow trout during the launch of the biofilter CWS for the use of polypropylene filler RK PLAST-control in it. According to the second variant, the clinical signs of adult rainbow trout were evaluated during the startup of the biofilter of the ultrasound scanner for the use of polypropylene filler RK PLAST in it and the addition of the microbiological starter of the biofilter "Filtronorm-D" – experiment. In the experimental group, the biofilter water was introduced by our microbiological starter, biofilter filler "Filtronorm-D", containing live cultures of nitrifying and denitrifying bacteria in the amount of 107 CFU/cm3.
On the 10th day of the experiment, after the application of the microbiological starter of the biofilter filler "Filtronorm-D",
approximately 5 % of the fish with the initial clinical signs of nitrite poisoning were detected. The fish for a long time stood motionless in the corners of the pool, they had the darkening of the body and the brown color of the gills. On the 15th day after the launch of the CWS and the use of "Filtronorm-D", changes in behavior were detected in most of the research objects, which may indicate an increase in the amount of nitrites in water and their effect on the body of trout. Approximately 25 % of fish for a long time stays motionless in the corners of the pool, the mobility of the whole fish has decreased, some individuals have risen to the surface of the water. Changes in pigmentation of the body were also found in approximately
10 % of the individuals. The body became darker, the fins were sealed, the gills got brown color. During this trial period, the mortality rate of the fish was 1,3 %. On the 20th day of the experiment, the signs of nitrite poisoning of fish became increasingly pronounced. Approximately 30 % of the fish showed the presence of darkening of the body and fins, the latter were sealed. In the same fish, the changed color of the gills was observed – from red to brown. In addition, during this period of research, the highest trout death was determined – 3,1 %, which is almost 2.4 times (p <0,05) higher than in the 15-day period. The period of ultrasound starting from the 20th to the 25th day was characterized by a decrease in the content of nitrites from 1,1 mg/dm3 to 0.6 mg/dm3. The process of reducing the content of nitrites in water affected the activity of fish – only about 20 % of trout swam in the upper layers of water. On the 30th day of the launch of the ultrasound with the use of the microbiological starter of the biofilter filler "Filtronorm-D" no signs of nitrite poisoning in rainbow trout were noted. The loss of fish in the indicated period was practically not marked. At the same time, during the application of polypropylene filler RK PLAST without the addition of a microbiological starter of the biofilter filler "Filtronorm D", the most problematic period for fish, in which it is most dying due to the effect of nitrites, is the period from 25 to 30 days.
Thus, the conducted researches prove that during the launch of ultrasound for the use of polypropylene filler RK PLAST in it and the addition of the microbiological starter of the biofilter fillter "Filtertronorm D" the most dangerous is the period from 15 to 20 days. During this period, the largest death of fish and signs of nitrite poisoning is recorded, which requires the implementation of veterinary and sanitary preventive measures to reduce the toxic effects of nitrites.
Key words: RAS, rainbow trout, biofilter, "Filtronorm-D", nitrites, fish behavior, signs of poisoning.
https://orcid.org/0000-0001-7430-94981. Grynevych, N.Ye. (2017). Otsinka toksychnosti mikrobiolohichnoho startera napovniuvacha biofiltra “Filtronorm D” dlia vyroshchuvannia raiduzhnoi foreli za pokaznykamy vyzhyvanosti ryb huppi ta infuzorii : NTB DNDKI vet. prep. ta korm. dob. [Toxicity Assessment of Microbiological Starter of Biofilter Filtronorm D Filler for Rainbow Trout Cultivation by Guppy and Infusion Survival Ratios: NTB DNCI Veterinary Drug and Feed Additives]. Lviv, Issue. 18, no. 2, pp. 219–224.
2. Grynevych, N.Ye. (2017). Vplyv mikrobiolohichnoho startera napovniuvacha biofiltra “Filtronorm D” na intensyvnist nitryfikuiuchykh protsesiv mikroflory reaktora biofiltra pid chas zapusku UZV dlia vyroshchuvannia raiduzhnoi foreli [Influence of microbiological starter of biofilter filler "Filtronorm D" on the intensity of nitrifying processes of the microflora of the biofilter reactor during the launch of the ultrasound for growing rainbow trout]. Agrarian Bulletin of the Black Sea Region: a collection of scientific works. Odessa, Veterinary science. Issue 83, pp. 33–38.
3. Shakhmurzov, M.M., Pryzenko, V.K. (1991). Otravlenie ryb nitritami v forelevyh hozjajstvah i organizacija mer bor'by s toksikozami [Fish nitrite poisoning in trout farms and the organization of measures to combat toxicosis]. Vtoraja vsesojuznaja konf. po c/h toksikologii: tezisy dokladov [The Second All-Union Conference on Agricultural Toxicology: Abstracts]. St. Petersburg, Vol. 2, pp. 265–266.
4. Shakhmurzov, M. M., Kazanchev, M. Kh., Hushchyn, V. N. (1998). Soderzhanie nitritov i nitratov v vode i rybe rybohozjajstvennyh vodoemov [The content of nitrites and nitrates in water and fish in fishery reservoirs]. Sbornik nauchnyh trudov VNII vet.san., gigieny i jekol.[ Collection of scientific papers of the All-Union Scientific Research Institute of Veterinary San., Hygiene and ecol.]. pp. 64–67.
5. Blancheton, J.P. (2002). Recent developments in recirculation systems. Seafarming today and tomorrow: Abstracts and extended communications of contributions presented at the International conference. Aquaculture Europe 2002, Trieste. Italy, pp. 3–9.
6. Attramadal, K.J.K. (2011). Water treatment as an approach to increase microbial control in the culture of cold water marine larvae – PhD Dissertation at the Department of Biology. Norwegian University of Science and Technology (NTNU).
7. Dalsgaard, J. (2013). Farming different species in RAS in Nordic countries: Current status and future perspectives. Aquacultural Engineering. Vol. 53, pp. 2–13.
8. Dolezalova, P., Macova, S., Pistekova, V., Svobodova, Z., Bedanova, I., Voslarova, E. (2011). Nitrite toxicity assessment in Danio rerio and Poecilia reticulata. Acta Veterinaria Brno. Vol. 80, pp. 309–312.
9. Chen, S., Ling, J., Blancheton, J.P. (2006). Nitrification kinetics of biofilm as affected by water quality factors. Aquacultural Engineering. Vol. 34, pp. 179–197.
10. Clauss, T., Dove, A., Arnold, J. (2008). Hematologic disorders of fish. The veterinary clinics of North America Exotic Animal Practice. Vol. 11, pp. 445–462.
11. Grynevych, N., Dyman, T., Kukhty, M., Semaniuk, N. (2017). Composition of psychrotrophic microflora of water and biofilter filler in recirculation aquaculture system on trout farm. Research Journal of Pharmaceutical, Biological and Chemical Sciences. Vol. 8(3), pp. 900–905.
12. Grynevych, N., Sliusarenko, A., Dyman, T., Sliusarenko, S., Gutyj, B., Kukhtyn, M., Hunchak, V., Kushnir, V. (2018). Ethiology and histopathological alterations in some body organs of juvenile rainbow trout Oncorhynchus mykiss (Walbaum, 1792) at nitrite poisoning. Ukrainian Journal of Ecology. no. 8 (1), pp. 402–408.
13. Hu, J., Li, D., Liu, Q., Tao, Y., He, X., Wang, X., Li, X., Gao, P. (2009). Effect of organic carbon on nitrification efficiency and community composition of nitrifying biofilms. Journal of Environmental Sciences. Vol. 21, pp. 387–394.
14. Itoi, S., Ebihara, N., Washio, S., Sugita, H. (2007). Nitrite-oxidizing bacteria, Nitrospira, distribution in the outer layer of the biofilm from filter materials of a recirculating water system for the goldfish Carassius auratus. Aquaculture. Vol. 264, pp. 297–308.
15. Itoi, S., Niki, A., Sugita, H. (2006). Changes in microbial communities associated with the conditioning of filter material in recirculating aquaculture systems of the pufferfish takifugu rubripes. Aquaculture. Vol. 256, pp. 287–295.
16. Kim, E.W., Bae, J.H. (2000). Alkalinity requirements and the possibility of simultaneous heterotrophic denitrification during sulfur-utilizing autotrophic denitrification. Water Science and Technology. Vol. 42, pp. 233–238.
17. Kroupová, H., Máchová, J., Svobodová, Z. (2005). Nitrite influence on fish – a review. Vet Med – Czech. Vol. 50, pp. 461–471.
18. Krumins, K., Ebeling, J.M., Wheaton, F. (2001). Ozones effects on power-law particle size distribution in recirculating aquaculture systems. Aquacultural Engineering. Vol. 25, pp. 13–24.
19. Lear, G., Anderson, M.J., Smith, J.P., Boxen, K., Lewis, G.D. (2008). Spatial and temporal heterogeneity of the bacterial communities in stream epilithic biofilms. FEMS Microbiology Ecology. Vol. 65, pp. 463–473.
20. Leonard, N., Guiraud, J.P., Gasset, E., Cailleres, J.P., Blancheton, J.P. (2002). Bacteria and nutrients – Nitrogen and carbon – In a recirculating system for sea bass production. Aquacultural Engineering. Vol. 26, pp. 111–127.
21. Lequette, Y., Boels, G., Clarisse, M., Faille, C. (2010). Using enzymes to remove biofilms of bacterial isolates sampled in the food-industry. Biofouling. The Journal of Bioadhesion and Biofilm Research. Vol. 26, no. 4, pp. 421–431.
22. Metaxa, E., Deviller, G., Pagand, P., Alliaume, C., Casellas, C., Blancheton, J.P. (2006). High rate algal pond treatment for water reuse in a marine fish recirculation system: Water purification and fish health. Aquaculture. Vol. 252, pp. 92–101.
23. Pulvenis, J.F. (2009). The State of World Fisheries and Aquaculture (SOFIA), FAO Fisheries and Aquaculture Department. Rome.
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