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Leptospirosis of the dogs in Kyiv in 2016-2018, serological monitoring and analysis of the ethioological structure

Leptospirosis – common to humans and animals is a dangerous infectious disease that is caused by microbes - leptospires. The disease is accompanied by fever, kidney damage, liver, cardiovascular and nervous system. Leptospirosis in dogs is considered one of the most common diseases. Particularly difficult is the breed with a faulty type of body structure, such as: Neapolitan Mastino, Bulmastiff, English Bulldog, French Bulldog, Boxer, Bologna, Bloodhound, Basset Hound. The disease is most often diagnosed in dogs of hunting breeds, as a result of frequent contact with standing water, as well as in courtyard and stray dogs. Young animals and puppies get sick more often, as they do not have a stable immunity, the hemorrhagic form is more often diagnosed in older dogs.

The subject was to study the distribution of leptospirosis among dogs, to establish the seropositivity level and to determine the seroprevalence of the most common of Leptospira serotypes that circulate among this species of animals.

For research, an extensive diagnostic series of L. interrogans which includes 20 serovars, and blood serum from dogs that were selected in veterinary clinics in the city of Kyiv, were used as antigen and were transferred to the laboratory of leptospirosis in agricultural animals from the Museum of Microorganisms of the Institute of Veterinary Medicine of the National Academy of Sciences of Ukraine.

Studies of blood serum were performed by the microagglutination test (MAT) followed by dark-field microscopy. PMA was placed in 4 dilutions: 1:50, 1: 100, 1: 500 and 1: 2500.

According to numerous publications of scientists from different countries of the world, the seroprevalence level of leptospirosis infection among the dogs varies from 39% to 95%.

A total of 1831 samples of blood serum were studied in the microscopic agglutination test. As a result of the serological study, 873 animals reacted positively, which is 47.7% of the total number of investigated ones.

Analyzing the registered antibody titers, which is most often found titer 1: 100, which is 50.4% of the total number of positive reactions. This indicates the presence of a disease in dogs.

Serrogroup Icterohaemorrhagiae is recorded in almost 50% of all positive reactions to leptospirosis and plays a major role in the etiology of the disease. It can be assumed that these dogs had contact with rats or their urine. In turn, the leading for these animals serogroup Canicola was detected in only a third of cases. Other serological groups played a minor role in the etiological structure.

Summing up the aforesaid, according to the results of our work, serological prevalence of the pathogenesis of leptospirosis among dogs was determined to be 47.7%. Was detected the circulation of Leptospira`s antibodies in blood serum of these animals. The analysis of the etiological structure of leptospirosis showed that the dominant serogroups were Icterohaemorrhagiae and Canicola. Seven serogroups (Pomona, Sejroe, Grippotyphosa, Australis, Autumnalis, Celledoni, Ballum) were recorded in the range of 1% to 4%. Other serogroups do not have a significant effect on the morbidity of dogsю

Keywords: leptospirosis, dogs, etiological structure, serological monitoring, antibody, microscopic agglutination test.

1. Durski, K. N., Jancloes, M., Chowdhary, T., Bertherat, E. (2014). A global, multi-disciplinary, multi-sectorial initiative to combat leptospirosis: Global Leptospirosis Environmental Action Network (GLEAN). International Journal of Environmental Research and Public Health, 11(6), pp. 6000–6008.  Retrieved from: DOI: 10.3390/ijerph110606000.

2. Schuller, S., Francey, T., Hartmann, K.  (2015). European consensus statement on leptospirosis in dogs and cats.  Journal of Small Animal Practice, pp. 159–179. Retrieved from: DOI: 10.1111/jsap.12328.

3. Raghavan, R., Brenner,  K., Higgins,  J. (2011). Evaluations of land cover risk factors for canine leptospirosis : 94 cases (2002 – 2009).  Preventive Veterinary Medicine. Elsevier B.V., Vol. 101 no. (3–4), pp. 241–249.  Retrieved from: DOI: 10.1016/j.prevetmed.2011.05.010.

4. Alton, G. D., Berke O., Reid-smith.  (2009). Increase in seroprevalence of canine leptospirosis and its risk factors , Ontario 1998 – 2006 Résumé. The Canadian Journal of Veterinary Research, Vol. 73, pp. 167–175.

5. Adler, B. (2015). Leptospira and Leptospirosis. Berlin, Heidelberg: Springer Berlin Heidelberg (Current Topics in Microbiology and Immunology), 295 p. Retrieved from: DOI: 10.1007/978-3-662-45059-8.

6. Ayral, F.C., Bicout, D.J., Pereira H. (2014).  Short report: Distribution of Leptospira serogroups in cattle herds and dogs in France. American Journal of Tropical Medicine and Hygiene,  Vol. 91(4), pp. 756–759. Retrieved from: DOI:  10.4269/ajtmh.13-0416.

7. Pratt, N., Conan, A., Rajeev, S. (2017). Leptospira Seroprevalence in Domestic Dogs and Cats on the Caribbean Island of Saint Kitts. Veterinary Medicine International, 2017 (November). Retrieved from: DOI:   10.1155/2017/5904757.

8. Mayer-Scholl, A., Luge, E., Draeger, A. (2013). Distribution of Leptospira Serogroups in Dogs from Berlin, Germany. Vector-Borne and Zoonotic Diseases, Vol. 13(3), pp. 200–202. Retrieved from: DOI: 10.1089/vbz.2012.1121.

9. Tagliabue, S., Figarolli M., D᾽Incau, M. (2016). Indagine sierologica sulla presenza di Leptospira spp. in Italia: Dati nazionali 2010-2011. Veterinaria Italiana, Vol. 52(2), pp. 129–138. Retrieved from: DOI: 10.12834/VetIt.58.169.2.

10. Suepaul, S. M., Carrington, C.V.F. (2010). Serovars of Leptospira isolated from dogs and rodents. Epidemiology and Infection, Vol. 138(7), pp. 1059–1070. Retrieved from: DOI: 10.1017/S0950268809990902.

11. Levett, P. N. (2001). Leptospirosis. Clinical microbiology reviews, Vol. 14(2), pp. 296–326. Retrieved from: DOI: 10.1128/CMR.14.2.296.

12. Kobayashi, Y. (2001). Discovery of the causative organism of Weil’s disease: Historical view.  Journal of Infection and Chemotherapy, Vol. 7(1), pp. 10–15. Retrieved from: DOI: 10.1007/s101560170028.

13. Thibeaux, R., Girault, D., Bierque, E. (2018). Biodiversity of Environmental Leptospira : Improving Identification and Revisiting the Diagnosis. Vol. 9 (May), pp. 1–14. Retrieved from: DOI: 10.3389/fmicb.2018.00816.

14.  Ko, A. I., Goarant, C., Picardeau, M. (2009). Leptospira: The dawn of the molecular genetics era for an emerging zoonotic pathogen. Nature Reviews Microbiology. Nature Publishing Group, Vol. 7(10), pp. 736–747. Retrieved from: DOI: 10.1038/nrmicro2208.

15. Picardeau, M. (2017). Virulence of the zoonotic agent of leptospirosis: Still terra incognita? Nature Reviews Microbiology. Nature Publishing Group, Vol. 15(5), pp. 297–307. Retrieved from: DOI: 10.1038/nrmicro.2017.5.

16. Soboleva, Gh. L., Nepoklonova, Y.V. Alyper, T.Y. (2013). Leptospyroz sobak [Leptospirosis of dogs]. Possyjskyj veterynarnyj zhurnal. Melkye domashnye y dykye zhyvotnye [Russian veterinary journal. Small domestic and wild animals], no. (3), pp. 6–10.

17. Greene, C. E. (2006). Infectious diseases of the dog and cat. Saunders/Elsevier. 1387 p.

18. Miotto, B.A., Guilloux, A.G.A., Tozzi, B.F. (2018). Prospective study of canine leptospirosis in shelter and stray dog populations: Identification of chronic carriers and different Leptospira species infecting dogs. PLoS ONE, Vol. 13(7), pp. 1–23. Retrieved from: DOI: 10.1371/journal.pone.0200384.

19. Zakeri, S., Khorami, N., Ganji Z.F. (2010). Leptospira wolffii, a potential new pathogenic Leptospira species detected in human, sheep and dog. Infection, Genetics and Evolution, Vol. 10(2), pp. 273–277. Retrieved from: DOI: 10.1016/j.meegid.2010.01.001.

20. Silva, E. F., Cerqueria, G,M., Seyffert, N. (2009). Leptospira noguchii and human and animal leptospirosis, Southern Brazil. Emerging infectious diseases. Centers for Disease Control and Prevention, Vol. 15(4), pp. 621–3. Retrieved from: DOI: 10.3201/eid1504.071669.

21. Ortega-Pacheco, A., Clin-Flores, R.F., Gutierrez-Blanco, E., Jimenez-Coello,  M. (2008a).  Frequency and type of renal lesions in dogs naturally infected with Leptospira species. Annals of the New York Academy of Sciences, Vol. 1149, pp. 270–274. Retrieved from: DOI: 10.1196/annals.1428.088.

22.  Richardson, D. J., Gauthier, J. L. (2003). A serosurvey of leptospirosis in Connecticut peridomestic wildlife. Vector borne and zoonotic diseases (Larchmont, N.Y.), Vol. 3(4), pp. 187–93. Retrieved from: DOI:  10.1089/153036603322662174.

23. Renaud, C., Andrews, S., Djelouadji, Z. (2013). Prevalence of the Leptospira serovars bratislava, grippotyphosa, mozdok and pomona in French dogs. Veterinary Journal. Elsevier Ltd, Vol. 196(1), pp. 126–127. Retrieved from: DOI: 10.1016/j.tvjl.2012.10.002.

24. Cole, J. R., Sangster, L. T., Sulzer, C. R. (1982). Infections with Encephalitozoon cuniculi and Leptospira interrogans, serovars grippotyphosa and ballum, in a kennel of foxhounds. Journal of the American Veterinary Medical Association, Vol. 180(4), pp. 435–7. Retrieved from: http://www.ncbi.nlm.nih.gov/pubmed/6800994 (Accessed: 13 February 2019).

25.  Arent, Z. J., Andrews, S., Adamama-Moraitou, K. (2013).  Emergence of novel Leptospira serovars: A need for adjusting vaccination policies for dogs ? Epidemiology and Infection, Vol. 141(6), pp. 1148–1153. Retrieved from: DOI:  10.1017/S0950268812002087.

26. Aljeksejeva, Gh.B. (2016). Metodychni rekomendaciji z laboratornoji diaghnostyky leptospirozu tvaryn. [Methodical recommendations for laboratory diagnosis of leptospirosis in animals]. Kyiv, DNDILDVSE, 34p.

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