Lymphoproliferative diseases to date, unfortunately, have become quite widespread, they are especially common among children, although among the adult population it is quite frequent pathology from diseases of the blood system. In recent years, a large number of oncology research, such as the study of the antitumor activity of a number of drugs, the study of carcinogenesis and molecular genetic aspects, have been conducted in vivo and in vitro. Most often as a biological model, rodents are used, and in particular – mice and rats. Directly in oncology, and in particular in oncohematology, the most widespread are the lines of mice such as Balb/c, C57/Bl, Nude. These lines have weakened immunity, or almost completely suppressed nominal system, which makes it relatively easy to implant a number of tumors, both homogeneous and heterogeneous (human).

The most successful transplantation of human tumors is performed in mice and rats with a mutation of nu. The nu mutation has multiple effects. Its main manifestations are absence of thymus and wool cover. Therefore, such animals are called nude – naked. Because of the absence of thymus, mice and rats nude develop immunodeficiency, as a result of which heterologous tumors have been successfully implanted in them.

In parallel with laboratory animals, it is of great importance to use cell cultures for a number of studies in oncohematology. In oncological practice, when studying lymphoproliferative diseases, both animal and human cell cultures are widely used. The most widespread of the animals oncocultures were L1210 (mouse, ascites fluid (lymphoblastic leukemia), P388D1 (DBA/2 mouse, lymphoid neoplasm), El-4 (dimethyl benzanthracene induced mouse lymphoma), P3X63Ag8.653 (BALB/c mouse, myeloma , clone of the line P3X63Ag8), NFS-60 (mouse, myeloid leukemia) and a number of other cultures.

Human cell cultures work intensively with: Daudi (lymphoma of Berkit), C8166 (human, T-lymphoblastic leukemia), CCRF-SB (human, acute lymphoblastic leukemia), CEM.NKR (human, T-lymphoblastic leukemia), CEM-SS (human, T-lymphoblastic leukemia), HL-60 (human, promyelocytic leukemia), IM-9 (human, myeloma), KJ-1 (human, acute myeloid leukemia), Jurkat (human, T-lymphoblastic leukemia), Jurkat -tat (human, T-lymphoblastic leukemia) and a number of other.

The aim of our work was the creation of biological models of lymphoproliferative diseases for further study of the factors affecting the development of the tumor and the development of early diagnosis of these diseases.

Materials and methods. In the experiment, non-native mice, linear mice BALB/c and C57/Black were used. Of oncoculture cells for transplantation (inoculation) of the tumor were used: L1210 (mouse lymphoblastic leukemia), P388D1 (lymphoid neoplasm).

Methods for introducing cell cultures to animals: intramuscular, subcutaneous, intraperitoneal administration was used. Intravenous and intracerebral introduction to small animals – rats and mice – is a rather laborious process, on the basis of this, we have chosen the methods described above. Their technique is quite simple, and it is relatively low-invasive methods of administration. The death of animals due to errors with the introduction of drugs is minimal, which makes it possible to widely use these methods in an experiment

Intramuscular injection: the animal was fixed in the abdominal position, or in an upright position and injected with an insulin syringe. The injection was made in the femoral muscle, from the inner surface of the thigh. Dose of administration: mice – no more than 0.5 ml of cell culture. Intraperitoneal route of administration: the animal was fixed in an upright position, the injection was performed near the white line (on the right or on the left side), in the lower third of the abdominal wall. The needle was pointed upwards. The dose of administration is 0.5 ml of cell suspension

Subcutaneous mode of administration: the animal was fixed in the abdominal position, the injection was performed in the area of ​​the scapula using an insulin syringe. Initially, carefully pulled the skin of the animal, then injected the needle at the base of the formed skin fold. Carefully move the needle to the right and left to make sure that it is under the skin, and not in the thickness of the muscles. Then, the cell culture was administered in a dose of 0.5 ml. Dose of introduction of cell cultures: in the experiment, dosages from 6 million cells to 10 million cells were used for administration to one animal.

Results of the research. Using linear animals, we reproduced an ascites tumor in BALB / c mice using a cell culture of L1210 (mouse lymphoblastic leukemia) at a dose of 10 million cells per animal with intraperitoneal injection. The experiment is 26 days long. The reliability of the results was confirmed by histological and cytological studies.

 In the experiment it was not possible to reproduce the tumor in the body of nonbreeding animals, with none of all the dosages and lines of cell cultures used.

Conclusions: the creation of a biological model of lymphoproliferative diseases is possible with the use of linear animals with weakened immunity (BALB / c mice) and corresponding cell lines (L1210 (mouse lymphoblastic leukemia)) at a rather high dosage (10 million cells per administration).

Keywords: oncohematology, biological models, laboratory animals, fixation, cell cultures.