You are here

Головна » 2020 » Scientific Journal of Veterinary Medicine 2'2020

The toxicokinetics of Bromine in the body of laying hens under conditions of subchronic administration of sodium bromide diet

The aim of the scientific work was to study the toxicokinetics of Bromine in the body of laying hens under conditions of subchronic administration of sodium bromide diet. The subject of the study was the content of bromine in the organs and tissues of laying hens under conditions of subchronic administration of sodium bromide diet. The experiment was on laying hens. Three experimental and one control groups of animals were formed (n=15). The background indicator of the Bromine content in the compound feed was 2.0 mg/kg of feed. An aqueous solution of sodium bromide was added daily to the feed of the experimental groups for 28 days, and the observation of the poultry was continued for the next 14 days. The poultry of the experimental groups received bromine with feed at a dose of 10.0 mg/kg, II – 50.0 mg/kg, III – 250.0 mg/kg of feed. The selection of organs was under conditions of euthanasia of the poultry, by total exsanguination, during inhalation chloroform anesthesia on days 14, 28 and 42 of the experiment, 5 animals per group. The following research methods were used in the work: clinical (daily examination of the poultry), pathological (dissection and sampling of organs for research), toxicological (determination of Bromine content using X-ray fluorescence analysis), statistical (processing of the results was carried out by methods of variation statistics using the Statistica 6.0 software ( StatSoft Inc., USA)). It was found that bromine is absorbed most intensively in the small intestine: on the 28th day of the experiment, its content exceeded the control in group I by 6.5 times, in group II – 22 times, in III – 201.1 times. The study of the content of bromine in the liver, spleen, heart, and brain of three experimental groups testifies to the "material" accumulation of the element. In addition to the digestive tract, the excretion of bromine from the body of laying hens occurred through the kidneys, and possibly with exhaled air. In group III, on the 28th day, its content in the kidneys was 23.1 times higher than the control indicator, and on the 14th day in the lungs, the excess of the control indicator was 27.4 times. The excess of the element was not excreted from the body within 14 days after the cessation of the intake of sodium bromide with food. Key words: Bromine, subchronic intake, laying hens, bromine content in organs, toxicokinetics, sodium bromide.

 

KORENEVA YU. https://orcid.org/0000-0001-9401-7732


  1. Derzhavnyj rejestr pestycydiv i agrohimikativ, dozvolenyh do vykorystannja v Ukrai'ni (rozpochato z 01.01.08 zgidno vymog postanovy Kabinetu Ministriv Ukrai'ny vid 21.11.2007 № 1328, onovleno 14.11.2019) [State Register of Pesticides and Agrochemicals Permitted for Use in Ukraine (started on January 1, 2008 in accordance with the requirements of the Resolution of the Cabinet of Ministers of Ukraine of November 21, 2007 № 1328, updated on November 14, 2019).]. 2019. Available at:https://menr. gov.ua/content/derzhavniy-reestr-pesticidiv-i-agrohimikativdozvolenih-do-vikoristannya-v-ukraini-dopovnennya-z01012017-zgidno-vimog-postanovi-kabinetu-ministrivukraini-vid-21112007--1328.html
  2. Wegman, R. C. C., Hamaker, Ph., de Heer, H. (1983). Bromideion balance of apolder district with large-scaleuse of methyl bromide for soil fumigation. Food and Chemical Toxicology. Vol. 21, no. 4, pp. 361–367. Available at: https:// doi.org/10.1016/0278-6915(83)90089-3
  3. Pavelka, S. (2004). Metabolism of Bromide and Its Interference with the Metabolism of Iodine. Physiol. Res. Vol. 53, pp. 81–90. ISSN 0862-8408
  4. Babicky, A., Pavelka, S., Vobecky, M. (2005). Biological Half-lives of Bromideand Sodium in the Rat AreConnected and Dependenton the PhysiologicalState. Biological Trace Element Research. Vol. 103, pp. 49–58. Available at:https://doi.org/10.1385/BTER:103:1:049
  5. Predel'no dopustimye koncentracii (PDK) himicheskih veshhestv v vode vodnyh ob#ektov hozjajstvenno-pit'evogo i kul'turno-bytovogo vodopol'zovanija. Gigienicheskie normativy. GN 2.1.5.1315-03 [Maximum permissible concentration (MPC) of chemical substances in the water of water bodies of household, drinking and cultural and household water use]. Available at: http://www.etch.ru/ norma.php?art=4
  6. Kucan, O.T., Orobchenko, O.L., Golubev M. І. (2015). Ekotoksikologіchna harakteristika bromu jak komponenta racіonіv dlja tvarin [Ecotoxicological characteristics of bromine as a component of animal diets]. Veterinarna medicina Ukraini [Veterinary medicine of Ukraine]. no. 5, pp. 24–27.
  7. Greve, P. A. (1983). Bromide-ionresidues in food and feed stuffs. Food and Chemical Toxicology. Vol. 21, no.4, pp. 357–359. Available at: https://doi.org/10.1016/0278- 6915(83)90088-1
  8. Rose, M., Miller, P., Baxter, M., Appleton, G., Crews, H., Croasdale, M. (2001). Bromine and iodine in 1997 UK total diet study samples. Journal of Environmental Monitoring. Vol. 3, no. 4, pp. 361–365. Available at: https:// doi.org/10.1039/b105695f
  9. Van Leeuwen, F. X. R., Sangster, B., Hildebrandt, A. G. (1987). The Toxicology of Bromide Ion. CRC Critical Reviews in Toxicology. Vol. 18, no. 3, pp. 189–213. Available at:https://doi.org/10.3109/10408448709089861
  10. Van Paemel, Marleen., Noël, Dierick., Geert, Janssens., Veerle, Fievez., Stefaan, de Smet. (2010). Selected Trace and Ultratrace Elements: Biological Role, Content in Feed and Requirements in Animal Nutrition – Elements for Risk. Assessment. EFSASupporting Publications. Vol. 7, no. 7. Available at:https://doi.org/10.2903/sp.efsa.2010.EN-68.
  11. Mineral tolerance of animals/ed. National Research Council of the National Academies. Washington: National Academies Press. 2005, 496 p.
  12. Du Toit, J., Casey, N. H. (2010). Effect of Bromine and Iodine in Drinking Water on Production Parameters of Broilers. South African Journal of Animal Science. Vol. 40, no. 4, pp. 301–310. Available at:https://doi.org/10.4314/sajas. v40i4.65238.
  13. Bihuzin, K.K. (1996). Brom i jod v pitanii brojlerov: avtoref. dis. na soiskanie uchenoj stepeni kand. s.-h. nauk: 06.02.02 [Bromine and iodine in broiler nutrition: Ph.D. thesis abstract for a Ph.D.]. Saransk, 23 p.
  14. Ageev, V. N. (1973). Kormlenie vysokoproduktivnyh jajcenoskih kur. [Feeding highly productive egg-laying hens]. Moskva: Kolos, 101 p.
  15. Kotsyumbas, I. Ya. (2006). Doklіnіchnі doslіdzhennja veterinarnih lіkars'kih zasobіv. [Preclinical studies of veterinary drugs]. Lviv: Triad Plus, 360 p.
  16. Levchenka, V.І. (2004). Klіnіchna dіagnostika vnutrіshnіh hvorob tvarin [Clinical diagnosis of internal diseases of animals]. Bila Tserkva, 608 p.
  17. Zharov, A.V., Ivanov, I.V., Strel'nikov, A.P. (2003). Vskrytye y patomorfologycheskaja dyagnostyka boleznej zhyvotnyh [Autopsy and pathomorphological diagnosis of animal diseases]. Moscow: KolosS, pp. 14–16.
  18. Kucan, O. T., Orobchenko, O. L., Kochergіn, Ju. A. (2014). Toksiko-bіohіmіchna harakteristika neorganіchnih elementіv ta zastosuvannja rentgenofluorescentnogo analіzu u veterinarnіj medicinіju [Toxico-biochemical characterization of inorganic elements and application of X-ray fluorescence analysis in veterinary medicine]. Kharkiv: Planet-print, 300 p.
  19. Du Toit, J., Casey, N. H. (2012). Iodine as an Alleviator of Bromine Toxicity in Thyroid, Liver and Kidney of Broiler Chickens. Livestock Science, Vol. 144, no. 3, pp. 269–274. Available at: https://doi.org/10.1016/j. livsci.2011.12.011.
AttachmentSize
PDF icon koreneva_2_2020.pdf470.48 KB
2020
Scientific Journal of Veterinary Medicine 2'2020