You are here

Головна » 2020 » Scientific Journal of Veterinary Medicine 1'2020

Number of T- and B-lymphocytes and their functional activity in the blood of common carps aff ected by Aeromonosis, and in the treatment of probiotic Enteronormin

Fish bacterial infectious diseases  are a signifi cant problem for aquaculture, often leading to massive fi sh deaths and signifi cant economic losses. For fi sh treatment often used antibacterial drugs: nitrofuran, antibiotics, feed antibiotics, as well 
as dyes. Finding eff ective drugs and studying their eff ects on the body of fi sh are relevant. Nowadays, diff erent groups of substances, including probiotics, are off ered as an alternative to increase the growth of animals and to combat pathogens. Here are the results of studies of the infl uence of the probiotic drug "Enteronormin" on the indicators that characterize the status of T- and B-cell immunity of carp aff ected by bacterial Aeromonosis. Enteronormin is a probiotic supplement , wich contains  a bacteria complex  - Enterococcus spp, Bacillus subtilis spp, Lactobacillus spp; and excipients - chitosan, peptone enzymatic and ground sugar (РП № ВВ-00427-02-12 від 13.04.2012). The research was conducted under the conditions of aquariums at the Lviv Research Station of the Institute of Fisheries of NAAS. The control group, which consisted of clinically healthy fi sh, received only 3% starch suspension, the fi rst experimental group (D1) - Aeromonosis aff ected carps who were given only 3% starch suspension, the second experimental group (D2), which consisted of carps aff ected Aeromonosis, through the probe within 7 days was administered the drug "Enteronormin", at the rate of 2 mg per 1 kg of fi sh weight, in the composition of 3% starch suspension. Before feeding for the carp drug was activated for 14-16 hours water enriched with Iodine and Selenium ions in the Jodis + Se form (TU U 15.7-30631018-011: 2011). It is established that the disease of carp on Aeromonosis leads to changes in the number and functional activity of immunocompetent blood cells. The inhibition of lymphocytogenesis and functional activity of immunocompetent cells in carp blood in a disease associated with the bacterial form of aeromonosis was ascertained. The use in the experimental carps group in the composition of 3% starch suspension of the drug "Enteronormin" had a normalizing eff ect on the state of T-cell specifi c protection, but did not signifi cantly aff ect the amount of EAC-RUL and their functional activity.

Key words: fi sh, carp, aeromonosis, probiotics, Enteronormin, T-lymphocytes, B-lymphocytes.

 

 

Solopova Kh. https://orcid.org/0000-0002-9772-7745


Vishchur O. https://orcid.org/0000-0003-4503-3896


  1. Vashhyk, Je.V.  (2018).   Antagonіstichna dіja probіotikіv shhodo P. aeruginosa ta zbudnikіv asocіjovanih bakterіozіv pticі [Antagonistic action of probiotics against P. aeruginosa and pathogens of associated bird bacteriosis]. Scientifi c and Technical Bulletin. Vol. 19, no 2, pp. 133–138.
  2. Vishchur, O.I., Kychun, I.V., Leshovska, N.M., Mamchuk, N.A., Yamroz, V.Y., Matlakh, I.I., Rokyta, I.M. (2008). Prirodna rezistentnіst' dejakih vidіv rib [Natural resistance of some fi sh species]. Nauk.-tehn. bjul. In-tu biologii' tvaryn ta Derzh. n.-d. kontrol. in-tu vetpreparativ ta korm. dobavok  [Scientifi c and technical bulletin of Institute of animal biology and State scientifi c research control institute of veterinary medical products and fodder additives]. Vol. 9, no. 3, pp. 343–347.
  3. Vovk, N.І., Bozhik, V.J. (2014). Іhtіopatologіja [Ichthyopathology]. Kyiv: Agricultural education, 308 p.
  4. Kondrateva, Y.A., Kytashova, A.A., Lanhe, M.A. (2011). Sovremennye predstavlenija ob immunnoj sisteme ryb [Modern ideas about the immune system of fi sh]. Organizacija immunnoj sistemy ryb  [Organization of the immune system of fi sh]. Bulletin of Moscow University Department of Physiology of Microorganisms, Faculty of Biology, Moscow State University M.V. Lomonosov]. Biology,  no. 4, pp. 11–23.
  5. Kotova, E.A., Pyshmanceva, N.A., Osepchuk, D.V., Pyshmanceva, A.A., Thakushinova, L.N. (2012) Probiotiki v akvakul'ture [Probiotics in aquaculture]. Sbornik nauchnyh trudov VNIIOK [Collection of scientifi c works of VNIIOK]. №1-1, pp. 100–103.
  6. Kotsiumbas, I. Ya., Hunchak, V.M., Stetsko, T.I. (2013). Problemi vikoristannja antimіkrobnih preparatіv dlja stimuljuvannja rostu produktivnih tvarin ta al'ternativi ih zastosuvannju [Problems with the use of antimicrobials to stimulate the growth of productive animals and alternatives to their use]. Naukovo-tehnichnyj bjuleten' Instytutu biologii' tvaryn i Derzhavnogo naukovo-doslidnogo kontrol'nogo instytutu vetpreparativ ta kormovyh dobavok  [Scientifi c and technical bulletin of Institute of animal biology and State scientifi c research control institute of veterinary medical products and fodder additives]. Vol. 14, no. 3–4, pp. 381–389.
  7. Ivanova, N.T. (1983). Atlas kletok krovi ryb [Atlas of fi sh blood cells]. Sravnitel'naja morfologija i klassifi kacija formennyh jelementov krovi ryb [Comparative morphology and classifi cation of fi sh blood cells]. Мoscow, 184 p.
  8. Mazur, T.V., Garkusha, I.Ye. (2016). Zmіni bіlkovih pokaznikіv krovі koropa za vikoristannja kompleksu simbіontnih mіkroorganіzmіv [Changes in the protein indices of carp blood during using a complex of symbiotic microorganisms]. Naukovij vіsnik L'vіvs'kogo nacіonal'nogo unіversitetu veterinarnoї medicini ta bіotehnologіj іmenі S. Z. Gzhic'kogo [Scientifi c Bulletin Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv]. Series: Veterinary Sciences. Vol. 18, no. 3, pp. 181–183.
  9. Petrov, R.V. (2014).  Bіologіchna cіnnіst' ta toksichnіst' m’jasa koropіv pri lіkuvannі ih vіd aeromonozu [Biological value and toxicity of carp meat in the treatment of aeromonosis]. Naukovyj visnyk L'vivs'kogo nacional'nogo universytetu veterynarnoi' medycyny ta biotehnologij im. G'zhyc'kogo. [Scientifi c Bulletin Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv]. Vol. 16, no. 2 (3), pp. 327–332.
  10. Petrov, R.V. (2014). Morfologіchnі ta hіmіchnі vlastivostі m’jasa koropa pri lіkuvannі vіd aeromonozu [Morphological and chemical properties of carp meat in the treatment of aeromonosis]. Vіsnik Sums'kogo nacіonal'nogo agrarnogo unіversitetu [Bulletin of Sumy National Agrarian University]. Serіja: Veterinarna medicina [Series: Veterinary Medicine]. Vol. 6, pp. 74–77.
  11. Briede, I. (2010). The prevalent bacterial fi sh diseases in fi sh hatcheries of Latvia. Environmental and Experimental Biology. Vol. 8, pp. 103–106.
  12. Claudiano, G.S., Gustavo, S. (2019). Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila. Fish & shellfi sh immunology. Vol. 88, pp. 259–265. Available at: https://doi. org/10.1016/j.fsi.2019.01.044
  13. Hai, N.V. (2015). The use of probiotics in aquaculture. Journal of applied microbiology. Vol. 119.4, рр. 917–935.  Available at: https://doi.org/10.1111/jam.12886
  14. Junior, G.B., de Freitas Souza, C., Descovi, S.N., Antoniazzi, A., Cargnelutti, J.F., Baldisserotto, B. (2019). Aeromonas hydrophila infection in silver catfi sh causes hyperlocomotion related to stress. Microbial pathogenesis. Vol. 132, pp. 261–265. Available at:https://doi. org/10.1016/j.micpath.2019.05.017
  15. Pandiyan, Priyadarshini. (2013). Probiotics in aquaculture. Drug Invention Today. Vol. 5.1, рр. 55–59. Doi:https://doi.org/10.1016/j.dit.2013.03.003
  16. Pérez‐Sánchez, Tania. (2014). Probiotics in aquaculture: a current assessment. Reviews in Aquaculture. Vol. 6(3), рр. 133–146. Available at:https://doi. org/10.1111/raq.12033
  17. Ronald, J. Roberts. (2012). Fish Pathology, 4th Edition. Wiley-Blackwell. 590 р.
  18. Tushak, S.F. (2018). Quantitative changes in hemogram of bees using probiotic «Enteronormin». Scientifi c Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences. Vol. 20.83, pp. 61–65.
AttachmentSize
PDF icon solopova_1_2020.pdf2.3 MB
2020
Scientific Journal of Veterinary Medicine 1'2020