You are here

Головна » 2020 » Scientific Journal of Veterinary Medicine 1'2020

Cytokine profi le in cows with necrobacterious fi ngers

Orthopedic diseases in cows signifi cantly reduce the profitability of dairy cattle breeding, causing signifi cant technological and economic damage. The aim of the study was to establish a cytokine profi le in cows with necrobacterioses of fi nger lesions. The work was performed in typical dairy farms, on cows with various clinical forms of purulent-necrotic lesions of the extremities of a necrobacterious nature. Diagnostic studies included a complex of clinical, bacteriological biopsy samples of purulent-necrotic foci and determination of the level of cytokines in the blood using enzyme-linked immunosorbent assay. A bacteriological study revealed various associations of F. necrophorum with E. coli, Clostridium spp., Diplococcus lans., Staphylococcus aureus and streptococci, which verifi ed the necrobacterious nature of limb lesions in cows. It was established that in cows with an acute course of purulent-necrotic infl ammatory process in the fi nger area, the concentrations of TNF-α and IL-10 sharply increase. The generalized form of necrobacillary lesions is characterized by a critical increase in the level of TNF-α in the blood by 16.8 times, IL-1β by 17.8 times compared with its level in the acute form of the concentration of IL-10. The cytokine index IL-10: TNF-α acquired a critical value of 0.4: 1, and IL-10 to IL-1β - 1: 1. The development of uncontrolled phlogogenic cytokinemia is noted, which refl ects a systemic infl ammatory response syndrome. A recurrent type of purulent-necrotic lesion of the extremities is characterized by rather low levels of IL-1β and IL-10. The content of IL-1β was 2.4 times greater (p <0.05). However, the concentration of TNF remained quite large and prevailed over the norm by 12.6 times (p <0.001), which was refl ected in the extremely low corresponding index of 0.3: 1. That is, in this case, in a chronic course of necrobacteriosis of the extremities of cows, uncompensated permanent cytokinemia due to TNF-α is formed, which leads to the recurrent nature of the purulent-necrotic process. So, the mechanism of development of the cytokine profi le in cattle requires further research. Key words: phlogogenic and anti-infl ammatory purulent-necrotic lesions, cytokines, cows, infl ammation, fi ngers.

 

M. Rublenko https://orcid.org/0000-0001-9690-9531


Melnikov V. https://orcid.org/0000-0003-2556-9001


  1. Stekolnykov, A.A. (2011). Zabolevanija konechnostej u krupnogo rogatogo skota pri intensivnom vedenii zhivotnovodstva, puti profi laktiki i lechenija: materialy Mezhdunarodnoj konferencii «Aktual'nye problemy veterinarnoj hirurgii» [Diseases of the extremities of cattle with intensive livestock, ways of prevention and treatment: proceedings of the International Conference “Actual Problems of Veterinary Surgery]. Ulyanovsk, pp. 3–7.
  2. Ermolaev, V. A., Maryn, E.M., Liashenko, P. M. (2016).  Dynamyka pokazatelei klynycheskoho analyza krovy u ortopedychesky bolnukh korov [Dynamics of indicators of a clinical blood test in orthopedic sick cows]. Vestnyk Altaiskoho hosudarstvennoho ahrarnoho unyversyteta [Bulletin of Altai State Agrarian University]. no. 10 (144), pp. 116–122.
  3. Hadžić, I., Pavlovic, I., Vojin, H., Gordana, A.B., Bojkovsli, J. (2013). Dermatitis interdigitalis and Dermatitis digitalis the Great Problem on Cattle Production. Bulletn UASVM. Veterinary Medicine. 70, pp. 242–248.
  4. Veremej, Je. (2011).  Bolezny roha - khlopot mnoho [Horn diseases - a lot of trouble]. Belorusskoe selskoe khoziaistvo [Belarusian agriculture]. no. 11, pp. 54–56.
  5. Veremej, Je. Y., Zhurba, V.A. (2003).  Rasprostranenye y profylaktyka zabolevanyi paltsev y koputtsev u krupnoho rohatoho skota [Distribution and prevention of diseases of fi ngers and hooves in cattle]. Veterynarnaia medytsyna Belarusy [Veterinary medicine of Belarus]. no. 2, pp. 33–35.
  6. Veremej, Je. Y. (2006). Ukhod za kopyttsamy vysokoproduktyvnoho molochnoho krupnoho rohatoho skota: praktycheskoe rukovodstvo [Hoof care of highly productive dairy cattle: a practical guide]. Vitebsk: UO VGAVM, 107 p.
  7. Maryn, E.M., Ermolaev, V.A., Ydohov, V.V.,  Sapozhnykov, A.V. (2009). Prirodnye drenirujushhie sorbenty pri gnojnyh pododermatitah u korov [Natural draining sorbents for purulent pododermatitis in cows]. Mezhduna- rodnyj vestnik veterinarii. [International Journal of Veterinary Medicine]. SPB, pp.13–16.
  8. Kozij V.I. Laminit u vysokoproduktyvnyh koriv (etiologija, patogenez, likuvannja i profi laktyka): avtoref. dys. d-ra vet. nauk: spec 16.00.05. Bila Cerkva, 2007. 36 s.
  9. Vlasenko, S.A.,  Rublenko, M.V., Chernyshenko, T.M., Hornytska, O.V., Platonova, T.M. (2016). Kharakterystyka koahuliatsiinykh protsesiv u koriv protiahom vahitnosti, pisliarodovoho periodu ta za akusherskoi y hinekolohichnoi patolohii [Characteristics of coagulation processes in cows during pregnancy, postpartum and obstetric and gynecological pathology]. Biolohiia tvaryn: nauk.-teoret. zhurnal [Animal biology: scientifi c theory. magazine]. Lviv, Vol. 18, no. 4, pp. 14–22.
  10. Vlasenko, S.A., Rublenko, M.V. (2012). Produktsiia oksydu azotu ta bilkiv hostroi fazy za hestatsiinoho protsesu, metrytu i ortopedychnoi patolohii u koriv [Production of nitric oxide and acute phase proteins during gestation, metritis and orthopedic pathology in cows]. Biolohiia tvaryn: nauk.-teoret. zhurnal [Animal biology: scientifi c theory. magazine]. Lviv, Vol. 14, no. 1–2, pp. 361–369.
  11. Vlasenko, S.A., Rublenko, M.V. (2009). Vmist okremykh tsytokiniv u krovi koriv z akusherskymy, hinekolohichnymy ta hniino-nekrotychnymy urazhenniamy v  diliantsi paltsia [The content of certain cytokines in the blood of cows with obstetric, gynecological and purulentnecrotic lesions in the fi nger area. Science]. Nauk. visnyk NUBiP Ukrainy [Bulletin of NULES of Ukraine]. Kyiv, Issue 136, pp. 289–294.
  12. Vlasenko, V.M., Rublenko, M.V., Ilnitskyi, M.H.  (2003). Poshyrennia zakhvoriuvan v diliantsi paltsiv u vysokoproduktyvnykh koriv zalezhno vid rivnia molochnoi produktyvnosti [The spread of diseases in the fi ngers in highly productive cows depending on the level of milk productivity]. Visnyk Bilotserkivskoho DAU [Bulletin of Bila Tserkva State Agrarian University]. Issue. 25, Part 1, pp. 45–51.
  13. Izdepskyi, V.I., Kulynych, S.M. (2006). Etiolohiia hniino-zapalnykh protsesiv v diliantsi paltsia u koriv u zymovo-stiilovyi period ta yikh likuvannia [Etiology of purulent-infl ammatory processes in the fi nger area in cows in the winter-stall period and their treatment]. Visnyk Bilotserkivskoho DAU [Bulletin of Bila Tserkva State Agrarian University]. Issue 4, pp. 47–55.
  14. Lopatyn, S.V. (2006). Optymyzatsyia systemы kontrolia эpyzootycheskoho protsessa nekrobakteryoza krupnoho rohatoho skota: dys. … dok. vet nauk: 16.00.03, 16.00.04. [Optimization of the control system for the epizootic process of necrobacteriosis in cattle: a dissertation of the Doctor of Veterinary Sciences: 16.00.03, 16.00.04.]. Novosibirsk, 325 p.
  15. Borysevych, V., Borysevych, B.,  Borysevych, V. (2007). Unhuloz – dystrofi ia kopytets velykoi rohatoi khudoby [Ungulosis is a dystrophy of the hooves of cattle]. Veterynarna medytsyna Ukrainy [Veterinary medicine of Ukraine]. no. 12, pp. 20–22.
  16. Higuchi, H., Nagahata, H. (2001). Relationsship betweenserum biotin concentration and moisture content of the sole horn in cows with clinical laminitis of sound hooves. Veter. Rec. Vol. 148, no. 7, pp. 209–210.
  17. Webster, A.J.F. (2002).  Eff ects of honsing practices on the development of foot lesions in dairy heifers in early lactatson. Veter. Rec. Vol. 151, no. 1, pp. 9–12.
  18. Khomyn, N.M. (2006). Aseptychni pododermatyty u velykoi rohatoi khudoby (etiolohiia, profi laktyka ta likuvannia: avtoref. dys. na zdobuttia nauk. stupenia doktora vet nauk: spets. 16.00.05. [Aseptic subdermatitis in cattle (etiology, prevention and treatment: author's dissertation for the degree of Doctor of Veterinary Sciences: special 16.00.05.]. Bila Tserkva, 36 p.
  19. Karpenko, L. Yu., Karpenko, A.N. (2008). Byokhymycheskye aspektы razvytyia kostno-sustavnoi patolohyy u korov [Biochemical aspects of the development of bone and joint pathology in cows]. Visnyk Bilotserkivskoho DAU [Bulletin of Bila Tserkva State Agrarian University]. Issue 56, pp. 71–75.
  20. Kutlukaev, Y.Y. (2003). Eff ektyvnost tkany VYON KN-1 v sochetanyy s antymykrobnыmy preparatamy pry lechenyy hnoino-nekrotycheskykh zabolevanyi paltsev u korov: dys…. kand. vet. nauk: 16.00.05. [Effi  ciency of fabric VION KN-1 in a combination to antimicrobic preparations at treatment of purulent-necrotic diseases of fi ngers at cows: the dissertation of the candidate of veterinary sciences: 16.00.05.]. Kazan, 150 p.
  21. Panasjuk S. D. Znachenie associacij mikroorganizmov v jetiologii i profi laktike infekcionnyh boleznej konechnostej krupnogo i melkogo rogatogo skota: nekrobakterioz, kopytnaja gnil': dis. … d-ra vet. nauk. Moskva, 2007. 470 s.
  22. Molokanov, V.A., Malov, D.V. (2009). Stymuliatsyia ymunnoho otveta pry nekrobakteryoze krupnoho rohatoho skota [Stimulation of the immune response in necrobacillosis of cattle]. Veterynaryia [Veterinary science]. no. 2, pp. 22–23.
  23. Borysevych, V.B., Sytiuk, V.H., Borysevych, B.V. (2009).  Ymmunokompleksnyi pododermatyt u korov [Immunocomplex pododermatitis in cows]. Veterynaryia [Veterinary Medicine]. no. 4, pp. 40–42.
  24. Stelmukhov, M.V. (2009). Jetiopatogeneticheskaja terapija gnojno-nekroticheskih jazv kopytec u korov: dis. … kand. vet. nauk: 16.00.05. [Etiopathogenetic therapy of purulent-necrotic ulcers of hooves in cows: the dissertation of the candidate of veterinary sciences: 16.00.05.]. St. Petersburg, 153 p.
  25. Tothova, C., Nagy, O., Seidel, H., Paulikova, I.  (2011). The infl uence of hoof diseases on the concentrations of some acute phase proteins and other variables of the protein profi le in heifers. Acta Veter. Vol. 61, no. 2–3, pp. 141–150.
  26. Jawori, P., Steinerz, S., Stefaniaki, T. (2008). Determination of selected acute proteins during the treatment of limb diseases in dairy cows. J. Vet. Med. Vol. 53, pp. 173–183.
  27. Cray, C., Zaias, J., Altman, N.H. (2009). Acute Phase Response in Animal. J. Comp. Med. Vol. 59, no. 6, pp. 517–526.
  28. Becker, J., Steiner, A., Kohler, S., Koller-Bahler, A., Wuthrich, M., Reist, M. (2014). Lameness and foot lesions in Swiss dairy cows: I. Prevalence. Schweiz. Arch. Tierheilkd. 156,  pp. 71–78.
  29. Refaai, W., van Aert, M., Abd El-Aal, A.M., Behery, A.E., Opsomer, G. (2013). Infectious diseases causing lameness in cattle with a main emphasis on digital dermatitis (Mortellaro disease). Livest. Sci. 156, pp. 53–63.
  30. Mills, J.A., Zarlenga, D.S. (2009) Age, segment, and horn disease aff ect expression of cytokines, growth factors, and receptors in the epidermis and dermis of the bovine claw. J Dairy Sci. 92, pp. 5977–5987.
  31. Trevisi, E., Jahan, N., Bertoni, G., Ferrari, A., Minuti, A. (2015). Pro-infl ammatory cytokine profi le in dairy cows: consequences for new lactation. Italian Journal of Animal Science. 14, pp. 285–292.
  32. Nagoev, B.S., Nagoeva, M.H., Kambachokova, Je.A. (2011). O roli citokinov v reguljacii immunnoj sistemy pri infekcionnyh zabolevanijah: materialy III Ezhegodnogo Vserossijskogo Kongressa po infekcionnym boleznjam (Moskva, 28-30 marta 2011 goda) [On the role of cytokines in the regulation of the immune system in infectious diseases: proceedings of the III Annual All-Russian Congress on Infectious Diseases (Moscow, March 28-30, 2011)]. Infekcionnye bolezni [Infectious diseases]. Vol. 9, application no. 1,  260 p.
  33. Odehov, E.S., Petrova, O. H. (2016). Analyz tsytokynovoho statusa pry ORVY krupnoho rohatoho skota [Analysis of cytokine status in acute respiratory viral infections in cattle]. Molodoi uchenui [Young scientist]. no. 6.5, pp. 89–91.
  34. Vlasenko, S.A. (2017). Patohenetychni mekhanizmy porushen reproduktyvnoi funktsii u vysokoproduktyvnykh koriv za hniino-nekrotychnykh urazhen v diliantsi paltsiv Dys… d-ra. vet. nauk : spets. 16.00.05. 16.00.07 [Pathogenetic mechanisms of reproductive dysfunction in highly productive cows with purulent-necrotic lesions in the fi ngers: doctor's dissertation. of veterinary sciences: 16.00.05. 16.00.07.].  Bila Tserkva,  429 p.
  35. Kuznetsov, A.P., Hriaznykh, A.V., Sazhyna, N.V. (2015). Fyzyolohyia ymmunnoi systemы: monohrafyia  [Physiology of the immune system: monograph]. Kurhan: Yzd-vo Kurhanskoho hos. un-ta [Kurgan: Publishing house of the Kurgan state. University]. 150 p.
  36. Gadek-Michalska, A., Bugajski, A.J., Bugajski, J.J. (2008).  Prostaglandins and interleukin-1beta in the hypothalamic-pituitary-adrenal response to systemic phenylephrine under basal and stress conditions. Physiol Pharmacol. Vol. 59(3), pp. 563–575.
  37. Schroecksnadel, K., Kaser, S., Ledochowski, M. (2003). Increased degradation of tryptophan in blood of patients with rheumatoid arthritis. J. Rheumatol. Vol. 30(9), pp. 1935–1939.
  38. Taylor, R.W. (1992). Sepsis, sepsis syndrome and septic shock. Philadelphia Lippincott. 537 p.
  39. Didion, S.P., Kinzenbaw, D.A., Schrader, L.I. (2009). Endogenous interleukin-10 inhibits angiotensin II- induced vascular. Hypertension. Vol. 54, no. 3, pp. 619–624.
  40. Asadullah, K., Sterry, W., Volk, H.D. (2003). Interleukin-10 therapyreview of new approach. Pharmacological reviews. Vol. 55, no. 2, pp. 241–269.
  41. Tinsley, J.H., South, S., Chiasson, V.I. (2010). Interleukin-10 reduces infl ammation, endothelial dysfunction, and blood pressure in hypertensive pregnant rats // Am. J. Physiol. Regul. Integr. Comp. Physiol. Vol. 298, no. 3, pp. 713–719.
  42. Rublenko, M.V. (2012). Tsytokinovyi status, reaktsiia hostroi fazy ta yii korektsiia za akusherskoi ta khirurhichnoi patolohii u produktyvnykh ta dribnykh domashnikh tvaryn [Cytokine status, acute phase reaction and its correction in obstetric and surgical pathology in productive and small domestic animals]. Bila Tserkva, 21p.
  43. Caruso, R. (2014).  NOD1 and NOD2: signaling, host defence, and infl ammatory disease. Immunity. Vol. 41, pp. 898–908.
AttachmentSize
PDF icon melnikovrublenko_1_2020.pdf5.46 MB
2020
Scientific Journal of Veterinary Medicine 1'2020