You are here

Diagnosis and differential diagnosis of viral hemorrhagic disease and eimeriosis of rabbits

The rabbit breeding industry in Ukraine is an important element of animal husbandry, most of the rabbits are in private households. In the absence of systematic veterinary care, accurate postmortem diagnosis of the causes of death of rabbits in households is important. Rabbit eimeriosis is widespread in Ukraine and with mixed intestinal and hepatic forms of eimeriosis against the background of unsatisfactory feeding and maintenance in young rabbits can cause death. Viral hemorrhagic disease of rabbits is also common in Ukraine and causes the death of rabbits of all ages. The aim was to study the pathomorphological changes in eimeria and viral hemorrhagic disease of rabbits and the use of other methods to confirm the diagnosis. The article presents the results of pathological, coprological and microbiological diagnosis of rabbit eimeriosis. Polymerase chain reaction was used to confirm the diagnosis of viral hemorrhagic disease of rabbits with the simultaneous establishment of the genotype of the pathogen. The effectiveness of the use of additional methods of postmortem diagnosis of eimeria (coprological, microscopic) and viral hemorrhagic disease of rabbits (polymerase chain reaction) was established. In rabbits that died from rabbit hemorrhagic disease the infection was caused by the virus of the first type.

Key words: pathological and anatomical diagnosis, coproscopy, PCR, liver damage.

  1. Vlasenko, I.V. (2012). Krolivnytstvo – rezerv v zabezpecheni prodovolchoi bezpeky derzhavy [Rabbit breeding – a reserve in ensuring food security of the state]. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S.Z. Gzhytskoho [Scientific Bulletin of Lviv National University of Veterinary Medicine and Biotechnology named after S.Z. Grzyckyi]. no. 2-4, pp. 15–18. (in Ukrainian).
  2. Gavrylina, O.G., Kolesnyk, A.A. (2016). Osoblyvosti laboratornoi diahnostyky eimeriozu kroliv [Features of laboratory diagnosis of rabbit eimeriosis]. Naukovo-tekhnichnyi biuleten Naukovo-doslidnoho tsentru biobezpeky ta ekolohichnoho kontroliu resursiv APK [Scientific and Technical Bulletin of the Research Center for Biosafety and Environmental Control of Agricultural Resources]. no. 4, pp. 55–58. (in Ukrainian).
  3. Kornienko, L.E. (2003). Infektsiini ta invaziini khvoroby kroliv [Infectious and invasive diseases of rabbits]. Bila Tserkva, 288 p. (in Ukrainian).
  4. Papchenko I.V. (2019). Patoloho-anatomichnyi roztyn trupiv silskohospodarskykh tvaryn z osnovamy sudovoi veterynarii: metodychni rekomendatsii dlia studentiv osvitnoho rivnia – mahistr ta slukhachiv Instytutu pisliadyplomnoho navchannia [Pathological and anatomical autopsy of farm animals with the basics of forensic veterinary medicine: methodical recommendations for students of educational level - master and students of the Institute of Postgraduate Studies]. Bila Tserkva, 47 p. (in Ukrainian).
  5. Papchenko, I.V. (2021). Patoloho-anatomichni zminy za eimeriozu kroliv: Materialy II Mizhnarodnoi naukovoi konferentsii «Tradytsiini ta innovatsiini pidkhody do naukovykh doslidzhen» [Pathological and anatomical changes in eimeriosis of rabbits: proceedings of the II International Scientific Conference "Traditional and innovative approaches to scientific research"]. Odesa (September 10, 2021): "European Scientific Platform". pp. 89–92. (in Ukrainian).
  6. Simonenko, M.V. (2002). Rozpovsiudzhennia ta klinichna kartyna pry eimeriozi kroliv [Distribution and clinical picture of rabbit eimeriosis]. Visnyk Bilotserkiv. derzh. ahrar. un-tu: zb. nauk. Prats [Bulletin of Bila Tserkva State Agrarian University: collection of scientific works]. Bila Tserkva, Issue 21, pp. 196–198. (in Ukrainian).
  7. Tvarynnytstvo Ukrainy 2020 [Livestock of Ukraine 2020]. Statystychnyi zbirnyk [Statistical collection]. Derzhana sluzhba statystyky [State Statistics Service]. 2021, pp. 53–59. (in Ukrainian).
  8. Franchuk, L.O. (2010). Poshyrennia ta formy perebihu zmishanoi eimerioznoi invazii u kroliv [Distribution and forms of mixed eimeria infection in rabbits]. Ahrarnyi visnyk Prychornomoria. Veterynarni nauky: zb. nauk. pr. [Agrarian Herald of the Black Sea region. Veterinary Sciences: collection of scientific articles]. Issue 56, pp. 148–153. (in Ukrainian).
  9. Abrantes, J., Lopes, A.M. (2021). A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV). Microorganisms. Vol. 9, no. 5, pp. 972–982. DOI:10.3390/ microorganisms9050972.
  10. Abrantes, J., van der Loo, W., Le Pendu, J., Esteves, P.J. (2012). Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV) a review. Vet. Res. Vol. 43, no. 1, pp. 12–22. DOI:10.1186/1297-9716-43-12.
  11. El-Akabawy, L.M., Zayan, K.A., Tantawy, A.A., Omar, Rem. (2004). Anticoccidial efficacy of propolis and toltrazuril against Eimeria stiedae in New Zealand white rabbit’s. Zag. Vet. J. Vol. 32, no.1, pp. 129–145.
  12. Hassan, K.M. (2016). Molecular diagnosis of Eimeria stiedae in hepatic tissue of experimentally infected rabbits. Exp. Parasitol. Vol. 169, pp. 1–5. DOI:10.1016/j.exppara.2016. 07.001.
  13. Kim, D.Y., Reilly, T.J., Schommer, S.K., Spagnoli, S.T. (2010). Rabbit tularemia and hepatic coccidiosis in wild rabbit. Emerg. Infect. Dis. Vol. 16, no. 12, pp. 2016–2017. DOI:10.3201/eid1612.101013.
  14. Martın, I. (2009). Polymerase chain reaction detection of rabbit DNA in food and animal feed. World Rabbit Science. no. 17, pp. 27–34. DOI:10.4995/ wrs.2009.667.
  15. Oncel, T., Gulegen, E., Senlik, B., Bakirci, S. (2011). Intestinal coccidiosis in angora rabbits (Oryctolagus cuniculus) caused by Eimeria intestinalis, Eimeria perforans and Eimeria coecicola. YYU Vet. Fak. Derg. no. 22, pp. 27–29.
  16. Sivajothi, S., Reddy, B.S., Rayulu, V.C. (2016). Study on impression smears of hepatic coccidiosis in rabbits. Journal of parasitic diseases: official organ of the Indian Society for Parasitology. Vol. 40, no. 3, pp. 906–909. DOI:10.1007/s12639-014-0602-8.
  17. Sivajothi, S., Reddy, B.S., Rayulu, V.C. (2013). Intestinal coccidiosis infection in domestic rabbits. Inter. J. Biol. Res. Vol. 2, no. 2, pp. 48–50.
  18. Velarde, R. (2017). Spillover Events of Infection of Brown Hares (Lepus europaeus) with Rabbit Haemorrhagic Disease Type 2 Virus (RHDV2) Caused Sporadic Cases of an European Brown Hare Syndrome-Like Disease in Italy and Spain. Transboundary and Emerging Diseases. Vol. 64, no.6, pp. 1750–1761. DOI:10.1111/tbed.12562.
AttachmentSize
PDF icon tsarenko_2_2022.pdf7.11 MB