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Histopathological changes of area of fracture of tubular bones at dogs under the influence of a preparation «Оstivet - I»
Influence research nanoaquahelats of metals: Mg, Co, Cu, Zn, Ag; on formation of an osteal callositas at dogs with fractures of
tubular bones with rather wide (2,0 - 4,0 mm) a fracture cleft. For the purpose of influence finding-out nanoaquahelats of metals on
histopathological changes of area of fracture have been applied a preparation «Оstivet - I» which contains aforementioned nanopar-
ticles in concentration of 100 mg / L, the size nanoparticles 1-50 nanometers. According to modern representations about the mecha-
nism reparative neogeneses of bones, the important role in an adnation of fragments play the components of an organic matrix pre-
sented first of all proteoglycans, glycoproteids, cationic proteins, collagen. Spent, applying an ocular-micrometer, measurements of
sizes of various osteal structures, histological and histochemical researches. Compared histopathological a picture of sites of fracture,
at dogs to the cross-section closed diaphyseal fractures of radial and tibial bones: In experience (n = 5) perorally daily within 3 weeks
set a preparation «Ostivet-I» (0,5 ml / kg); in each control (n = 5) animals received the same quantity of water. For this purpose a
method economical trepanobiopsy received small fragments from different sites of a cleft of fracture of radial and tibial bones, with
observance of norms of bioethics, since 10th till 25th day, with an interval of 5 days. Fragments fixed in 10 % th neutral formalin, a
decalcification spent to 7 % to nitric acid.
Received the frozen and paraffinic sections which painted a hematoxylin eosine, picrofucsin on Van Gizon, bromphenol
dark blue, alcin dark blue, spent the PASS-reaction.
Quantity of biopolymers (proteoglycans, glycoproteids, cationic proteins, collagen) estimated visually on intensity of
corresponding histochemical reactions in points: 1 - insignificant linkage of a stain, 2 - the expressed linkage of a stain,
3 - intensive linkage of a stain, 4 - the greatest possible linkage of a stain.
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The most typical changes of an organic basis of an osteal callositas are taped on 14 - 15 days. At control animals rather wide
cleft of fracture is filled rather with a matrix considerable quantity, in which structure are visualised in a considerable quantity, if to
judge on intensity of histochemical reaction, proteoglycans. Quantity of glycoproteids at a visual estimation of intensity a PASS-
reaction rather small. Accumulation in the fracture gap proteoglycans at dogs of control group leads to a formation of a significant
amount of a cartilaginous tissue. The superfluous quantity of a cartilaginous tissue slows down transformations of a soft callositas to
a firm osteal callositas and by that the definitive adnation of osteal fragments definitely brakes.
At dogs with the fractures, receiving nanoaquahelats of metals, in fracture gap considerably smaller quantity proteoglycans
compounds and considerably larger quantity of glycoproteids, cationic proteins and, that is especially important, the osteal collagen,
capable to attach to itself crystals of calcium phosphate (mineralization), is thus expressed formation of a soft callositas is intensified.
In a tissue fracture gap are formed the expressed vascular channels which researches for the given period yet are not filled bone
marrow regenerate but which are activly occupied further by elements of an osteal brain.
In a zone of fracture against dystrophic and necrobiotic changes of the extremities of fragments observed plentiful infil-
tration by neutrophils, monocytes and lymphocytes which in the control through 16 - 20 days the expressed proliferation of
fibrocytes. Last was accompanied by manufacture of the basic substance (matrix) of a connecting tissue. In experience re-
spective alterations were accelerated on 3 - 5 days then in a fracture gap noted an intensive proliferation of a grid of fibrous
fibers. In the control through 22 - 25 days accurately generated tissue of an osteal callositas with the made narrower vascular
channels was formed. The fracture callus acyanotically was painted over by a hematoxylin and eosine because of to the ex-
pressed sclerosis and a hyalinosis. In experience on 19 - 20 day fracture callus took a form of a dense osteal tissue which on
the density much more exceeded density of the extremities of osteal fragments which it strongly bridged among themselves
that marked formation firm osteal callous.
At formation fracture callus after 14 days of application nanoaquahelats of metals considerably the maintenance
proteoglycans decreased (on 24,3 %) at simultaneous augmentation of the maintenance of glycoproteids (on 24,7 %), cationic
proteins (in 2,09 times), collagen (in 1,99 times). These indicators confirm intensity of connective tissue neogenesis in
fracture gap, and also the phenomenon of the accelerated formation of a soft callositas under influence nanoaquahelats of
metals. Thus peroral application of a preparation «Ostivet - I» on 3 - 5 days accelerated formation of a stable firm osteal
callositas.
Keywords: nanoaquahelats of metals, fractures of tubular bones, osteal callositas, dogs.