You are here

Головна » 2020 » Scientific Journal of Veterinary Medicine 1'2020

Morphogenesis of the centers of ossifi cation of bone organs of piglets in the early postnatal period of ontogenesis

The dynamics of growth and development of the basic and additional centers of ossifi cation of bone organs gives an opportunity to fi nd out the patterns of change of their tissue components and subsequent age-related remodeling when performing biomechanical and hematopoietic functions. Features of morphogenesis of hematopoietic components of the skeleton in productive animals should be taken into account in determining the etiopathogenesis and development of methods of treatment and prevention of infectious diseases, immunodefi ciency and anemic conditions in young animals. Were studied bone organs (9th thoracic vertebra, femur) from clinically healthy newborns, 5-, 10-, 15- and 20-day-old pigs (n = 5) of white Ukrainian breed grown using traditional technologies. Radiological research in the organs of universal hematopoiesis determined the availability and degree of development of the dia-, epi- and apophysar centers of ossifi cation, as well as features of the structure of spongy and compact bone tissue. Determination of the relative area of the centers of ossifi cation on radiograph was performed by the method of “point counting” using the eyepiece test systems according to the method of G.G. Avtandilova. In newborn piglets, the bones of the axial skeleton show only the diaphysar (body) centers of ossifi cation and brackets, whereas in the bones of the peripheral bone, the diaphysar and epiphysar ones, as well as the apophysar (larger swivel). With age, the number of limb bones does not change, and the axial skeleton, except for previously formed diaphysar and brackets, appear epiphysar (heads and fossae), starting at the age of 20 days. In all bones there is an increase in the size and relative area of   previously formed centers of ossifi cation. In bone organs of all age groups, small-cell spongy bone prevails, whereas compact at the moment of birth, it is present only in the peripheral skeleton, and in axial - it fi rst appears in the body of a vertebra of 10-day piglets and also in the brackets - 20-day piglets. The thickness of metaphysar cartilages, subchondral bones of articular cartilages, as well as the epi- and diametaphysar subchondral bones increases with age.

Key words: blood forming and immune protection organs, skeleton, bone organs, basic and additional centers of ossifi cation, bone tissue, piglets. 

Oliyar A. https://orcid.org/0000-0002-8918-2693


Lieshchova M. https://orcid.org/0000-0002-4251-4152


Logvinova V. https://orcid.org/ 0000-0002-2084-6850


  1. Babaeva, A. H. (1987). Krovetvornie і lymfoydnie orhani. Strukturnie osnovi adaptatsyy і kompensatsyy narushennikh funktsyi [Haematopoietic and lymphoid organs. Structural bases of adaptation and compensation of impaired functions]. Moscow, 215 р.
  2. Dontsov, V. Y. (1990). Immunobiologiya postnatalnogo razvitiya [Immunobiology of postnatal development]. Moscow, Science, 152 р.
  3. Snetkova, P. O. (2010). Morfofunktsionalni osoblyvosti kistkovoi systemy sobak neonatalnoho ta molochnoho periodiv: avtoref. dys. ... dokt. vet. nauk: 16.00.02; Natsionalnyi universytet bioresursiv i pryrodokorystuvannia Ukrainy [Morphological and functional features of the neonatal and milk period dogs system of dogs: abstract dіs. ... cand. vet. sciences: 16.00.02; National university bioresource and environmental management of Ukraine]. Kiyv, 23 р.
  4. Sosonniy, S. V. (2012). Zakonomirnosti morfohenezu krovotvornykh komponentiv skeleta plodiv velykoi rohatoi khudoby: avtoref. dys. ... kand. vet. nauk: 16.00.02; [The law of morphogenesis of the blood-creating components of the skeleton of the fetus of neat: abstract dis. ... cand. vet. sciences: 16.00.02]. Kharkivska derzhavna zooveterynarna akademiia [Kharkivska State Veterinary Academy]. Kharkiv, 23 p.
  5. Gavrilin, P. М., Peretyatko, O. V. (2012). Osoblyvosti postnatalnoho morfohenezu ta strukturno-funktsionalnoi spetsializatsii parenkhimy limfatychnykh vuzliv ptytsi [Peculiarities of postnatal morphogenesis and structurally functional special parenchyma of lymphatic nodes in birds]. Naukovo-tekhnichnyi biuleten NDTs biobezpeky ta ekolohichnoho kontroliu resursiv APK [Science and technology bulletin of NDC for environmental protection and environmental control of agricultural resources]. Vol. 1, no. 1, рр. 16-22. Available at:http://base.dnsgb.com.ua/fi les/journal/ Buleten-boobezpeky/Bulet-biobezpeky-2012-%D0%A21%E2%84%961/Bulet-biobezpeky-2012-%D0%A21%E2%84 %961_%D1%81%D1%82%D0%BE%D1%80.%2016-22.pdf
  6. Szpinda, М.,  Baumgart, М., Szpinda, А., Woźniak, А., Mila-Kierzenkowska, С. (2013).New patterns of the growing L3 vertebra and its 3 ossifi cation centers in human fetuses – a CT, digital, and statistical study. Med Sci Monit Basic Res. Vol. 19, рр. 169–180. Available at:https://doi.org/10.12659/ MSMBR.883956
  7. Barsukova, V. V. (2013). Osoblyvosti imunnykh struktur tonkoi kyshky muskusnykh kachok v rannomu postnatalnomu ontohenezi [Features of immune structures of the small intestine of musky ducks in early postnatal ontogeny]. Naukovyi visnyk Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy]. [Scientifi c Bulletin of the National university bioresource and environmental management of Ukraine]. Seriia: «Veterynarna medytsyna, yakist i bezpeka produktsii tvarynnytstva» [Series: «Veterinary medicine, quality and safety of animal husbandry products»]. Vol. 188 (1), pp. 43–49. Available at:https://dspace.dsau.dp.ua/jspui/handle/123456789/1877
  8. Szpinda, M., Baumgart, M., Szpinda, A., Woz´niak, A., Małkowski, B., Wis´niewski, M., Mila-Kierzenkowska, C., Kro´liczewski, D. (2013).Cross-sectional study of the ossifi cation center of the C1–S5 vertebral bodies. Surg Radiol Anat. Vol. 35, pp. 395–402. Available at:https://doi.org/10.1007/ s00276-012-1045-5
  9. Skórzewska, А., Grzymisławska, M., Bruska, M., Łupicka, J., Woźniak, W. (2013). Ossifi cation of the vertebral column in human foetuses: histological and computed tomography studies. Folia Morphol. 72(3), pp. 230–238 Available at:https://doi.org/10.5603/FM.2013.0038
  10. Mіrnyi, O. M. (2013). Osoblyvosti dynamiky rostu ta rozvytku oseredkiv enkhondralnoho osteohistohenezu ta universalnoho hemopoezu v skeleti plodiv svyni sviiskoi [Peculiarities of the dynamics of growth and development of centers of endchondral osteogistogenesis and universal hematopoiesis in the skeleton of fetus pig domestic]. Naukovi pratsi Pivdennoho fi lialu Natsionalnoho universytetu bioresursiv i pryrodokorystuvannia Ukrainy «Krymskyi ahrotekhnolohichnyi universytet». [Scientifi c works of the Southern fi liation of the National university bioresource and environmental management of Ukraine «Crimean Agrotechnological University»]. Seriia: Veterynarni nauky [Series: Veterinary sciences]. Issue 151, pp. 294–298. Available at:http://nbuv.gov.ua/UJRN/Npkau_2013_151_52
  11. Rahmoune, D.E. (2016). Zakonomirnosti strukturno-funktsionalnoi orhanizatsii i zonalnoi spetsializatsii parenkhimy limfatychnykh vuzliv verbliuda sviiskoho (Camelus Dromedarius): avtoref. dys. ... kand. vet. nauk: 16.00.02 [Regularity of structural and functional organization and zonal specialization of the camel domestic lymph node parenchyma (Camelus Dromedarius): abstract dis. ... cand. vet. sciences: 16.00.02]. Kharkivska derzhavna zooveterynarna akademiia [Kharkivska State Veterinary Academy]. Kharkiv, 26 p.
  12. Sapin, M.R., Etingen, L. E. (1996). Immunnaya sistema cheloveka [Human immune system]. Moscow, Medicine, 304 р. Available at:https://www.libex.ru/detail/book41612.html
  13. Rabson, A., Rojt, A., Delvz, P. (2006). Osnovy medicinskoj immunologii [Fundamentals of Medical Immunology]. Moscow: Peace, 320 р. Available at:https://www.twirpx. com/fi le/1498199/
  14. Chertkov, I.L. (1978). Stvolovaya krovetvornaya kletka i eyo diff erencirovka v mieloidnom i limfoidnom napravlenii. Immunogenez i kletochnaya diff erencirovka [Hematopoietic stem cell and its diff erentiation in myeloid and lymphoid directions. Immunogenesis and cell diff erentiation]. Moscow, рр. 102–127.
  15. Evert, V. V., Gavrilin, P.M., Lieshchova, M. O. (2018). Morfometrychna kharakterystyka orhaniv universalnoho hemopoezu porosiat u period postnatalnoi adaptatsii [Morphometric characterization of universal hematopoietic organs in piglets during postnatal adaptation]. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S.Z. Gzhytskoho. Seriia: Veterynarni nauky [Scientifi c Bulletin Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv]. Series: Veterinary sciences. 20(83), рр. 13–18.  Available at:https://doi.org/10.15421/nvlvet8303
  16. Friedenstein, A.Ya. (1982). Stromalnye kletki kostnogo mozga i krovetvornoe mikrookruzhenie [Bone marrow stromal cells and hematopoietic microenvironment]. Arhiv patologii [Pathology Archive]. Vol. 44. no.10, рр. 3-11.
  17. Torubarova, N. A., Koshel, I. V., Yatsyk, G. V. (1993). Krovetvorenie ploda i novorozhdennogo [Hematopoiesis of the fetus and newborn]. Moscow, Medicine, 208 р.  Available at:http://patrick-book.ru/torubarova-n.-a.,-koshel-i.-v.,-yac
  18. Avtandilov, G.G. (1990). Medicinskaya morfometriya [Medical morphometry]. Moscow, Medicine, 384 р. Available at:https://www.twirpx.com/fi le/662076/
  19. Bajmishev, H. B., Krishtoforova, B. V., Lemeshhenko, V. V., Stegnej, Zh. G., Hrustaleva, I. V. (2013). Biologicheskie osnovy veterinarnoj neonatologii [Biological bases of veterinary neonatology]. Samara, RIC SGSHA, 452 р. Available at:https://rucont.ru/efd/231941
  20. Mіrnyi, O. M., Oliyar, А. V. (2014). Zakonomirnosti morfohenezu krovotvornykh komponentiv orhaniv universalnoho krovotvorennia ta imunnoho zakhystu v plodiv svyni sviiskoi [The law of morphogenesis hematopoietic components haematogenesis universal and immune protection organs of the domestic fetuses pig]. Naukovo-tekhnichnyi biuleten Naukovo-doslidnoho tsentru biobezpeky ta ekolohichnoho kontroliu resursiv APK [Science and technology bulletin of NDC for environmental protection and environmental control of agricultural resources]. Vol. 2, no.1, рр. 37–43. Available at:http://nbuv.gov.ua/UJRN/ndbnndc_2014_2_1_7
  21. Olіyar, A.V., Lieshchova, M.O. (2019). Strukturnofunktsionalna orhanizatsiia tsentralnykh orhaniv krovotvorennia ta imunnoho zakhystu porosiat protiahom rannoho postnatalnoho periodu ontohenezu [Structural and functional organization of central organs of hematopoiesis and immune protection of piglets during the early postnatal period of ontogenesis]. Theoretical and Applied Veterinary Medicine. Vol. 7. no.1, рр. 8–13. Available at:https://doi. org/10.32819/2019.71002
  22. Gavrilin, P., Oliyar, A., Myrnyi, O. (2016). Peculiarities of morphogenesis of universal hematopoesis and immune protection in fetuses of domestic pig. The Animal Biology. Vol. 18, no. 4, рр. 30-34. Available at:http://doi.org/10.15407/ animbiol18.04.030
  23. Gavrilin, P. M., Lieshchova, М. O., Evert, V. V., Myrnyi, O. M. (2017). Strukturno-funktsionalna orhanizatsiia kistkovoho mozku porosiat [The structural and functional organization of piglets` bone marrow]. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii imeni S.Z. Gzhytskoho [Scientifi c Bulletin Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv] Vol.19, no. 77, pp. 32–37. Available at:https://doi.org/10.15421/nvlvet7708
  24. Grabchak, Zh. G. (2000). Morfofunkcionalnye osobennosti bedrennyh kostej neonatalnyh telyat [Morphofunctional features of the femurs of neonatal calves]. Veterynarna medytsyna: Mizhvidomchyi tematychnyi naukovyi  zbirnyk [Veterinary medicine: Іnterdepartmental themed science compilation]. Issue 77, pp. 106–111.
  25. Travlos, G. S. (2006). Normal Structure, Function, and Histology of the Bone Marrow. Toxicologic Pathology. 34(5), рр. 548–565. Available at: https://doi. org/10.1080/01926230600939856 
  26. Baumgart, M., Szpinda, M., Szpinda, A. (2012). New anatomical data on the growing C4 vertebra and its three ossifi cation centers in human fetuses. Surgical and Radiologic Anatomy. Vol. 35(3), рр. 191–203. https://link.springer.com/ article/10.1007/s00276-012-1022-z
  27. Szpinda, M., Baumgart, M., Szpinda, A., Woźniak, A., Mila-Kierzenkowska, C., Dombek, M., Kosiński, A., Grzybiak, M. (2013). Morphometric study of the T6 vertebra and its three ossifi cation centers in the human fetus. Surgical and Radiologic Anatomy. Vol.35(10), pp. 901–916. Available at: https://cyberleninka.org/article/n/303435/viewer
  28. Otsuru, S., Desbourdes, L., J. Guess, A., J. Hofmann, T., Relation, T., Kaito, T., Dominici, M., Iwamoto, M., M. Horwitz, E. (2017). Extracellular vesicles released from mesenchymal stromal cells stimulate bone growth in osteogenesis imperfecta. Cytotherapy. рр. 1–12. Available at:https://doi. org/10.1016/j.jcyt.2017.09.012
  29. Otsuru, S., M. Overholt, К., S. Olson, Т., J. Hofmann, T., J. Guess, A., M. Velazquez, V., Kaito, T., Dominici, M., M. Horwitz, E. (2017). Hematopoietic derived cells do not contribute to osteogenesis as osteoblasts. Bone. Vol. 94, рр. 1–9. Available at:http://doi.org/10.1016/j.bone.2016.10.003
  30. Araby, N., Soliman, S., Raheem, E.A., Ahmed, Y. (2018). Morphogenesis of the sternum in quail embryos. SVU-International Journal of Veterinary Sciences. Vol. 1(1), pp. 16–24. Available at: https://www.semanticscholar.org/paper/Morphogenesis-of-the-Sternum-in-Qu...
AttachmentSize
PDF icon olijar_1_2020.pdf5.42 MB
2020
Scientific Journal of Veterinary Medicine 1'2020