You are here

Morphological and biochemical parameters of the blood of broiler chickens with ornithobacteriosis, and for the use of Poledoxin and Tilmox 25%

The article presents the results of studies of morphological and biochemical parameters of blood of broiler chickens KOBB-500, patients with ornithobacteriosis, who were not treated, and sick birds, for the treatment of which were used drugs "Poledoxin" (active substance - doxycycline hyclate) and "Tilmox 25%" (active substance - tilmicosin phosphate). Studies have shown that ornithobacteriosis in broiler chickens is characterized by erythrocytopenia, hypohemoglobinemia and decreased hematocrit. In the birds of the experimental groups, which in accordance with the treatment regimen used poledoxin and tilmox 25% erythrocyte count, hemoglobin content in the blood and hematocrit at 216 h of the experiment were higher than in the control: erythrocyte count - 19 and 36%, respectively; hemoglobin content - by 17% in poultry of both experimental groups; hematocrit - by 19 and 23%, respectively (p≤0.05). In the serum proteins of patients with ornithobacteriosis of broiler chickens of the control group was found dysproteinemia, in which at 216 h of the experiment there was a decrease in total protein and albumin by 12 and 34%, respectively. At the same time there was an increase in the content of globulins by 32%. The content of total protein and albumin in the serum of poultry 2 and 3 experimental groups increased and by 216 h exceeded the control index by 24 and 33% (p≤0.05), respectively, albumin - by 56% in poultry of both experimental groups. The content of globulins in the serum of poultry of the experimental groups for the period from 24 to 216 h of the experiment was in the range of 10.72 - 11.98 g / l, while in the control - 11.32 - 15.21 g / l. In the serum of broiler chickens of the control group for the period from 24 to 216 h increased the activity of alanine aminotransferase by 14%, while in the serum of poultry of experimental groups treated with poledoxin and tilmox 25% its activity decreased by 96, 120 and 216 h was probably less than in the control. Hypocalcemia was observed in the control group of broiler chickens ornithobacteriosis, whereas in the serum of poultry 2 and 3 experimental groups treated, the total calcium content in the period from 24 to 216 h was probably higher than in the control. Hypophosphatemia was observed in the control and 2 experimental birds (poledoxine), and the inorganic phosphorus content in their serum was 216 h lower than in the experimental group 3 birds (tilmox 25% was used) by 43 and 23%, respectively.

Key words: doxycycline, tilmicosin, respiratory diseases of poultry, O. Rhinotracheale, antibiotics, bioavailability.

  1. Devriese, L.A., De Herdt, P., Haesebrouck, F. (2010). Antibiotic sensitivity and resistance in Ornithobacterium rhinotracheale strains from Belgian broiler chickens. Avian Pathology. Vol. 30(3), pp. 197–200. Doi:10.1080/03079450120054596.
  2. Barbosa, E.V., Cardoso, C.V., Silva, R. (2020). Ornithobacterium rhinotracheale: An Update Review about An Emerging Poultry Pathogen. Veterinary Science. Vol. 7(1), pp. 1–13. Doi:10.3390/vetsci7010003.
  3. Gavrilović, P., Gavrilović, A., Vidanović, D. (2015). Comparative pathomorphological, bacteriological and serological examination of broiler breeders and pheasants experimentally infected with Ornithobacterium rhinotracheale. Avian Pathology. Vol. 45(5), pp. 513–519. Doi: 10.1080/03079457.2016.1168514
  4. Tyshkivska, A.M., Dukhnytskyi, V.B., Tyshkivskyi, M.Ia. (2020). Vplyv polodoksynu ta tylmoksu 25 % na morfolohichni ta biokhimichni pokaznyky krovi kurchat-broileri [The effect of poliodoxin and tilmox 25 % on the morphological and biochemical parameters of the blood of broiler chickens]. Naukovyi visnyk veterynarnoi medytsyny [Scientific Bulletin of Veterinary Medicine]. Bilotserkivskyi natsionalnyi ahrarnyi universytet [Bila Tserkva National Agrarian University]. no. 1:154, pp. 72–80. Doi:10.33245/2310-4902-2020-154-1-72-79
  5. Tyshkivska, A.M., Dukhnytsky, V.B., Tyshkivskyi, M.Ia. (2020). Nadkhodzhennia ta rozpodil doksytsyklinu v orhanizmi kurchat-broileriv [Receipt and distribution of doxycycline in broiler chickens]. Naukovyi visnyk veterynarnoi medytsyny [Scientific Bulletin of Veterinary Medicine]. Bilotserkivskyi natsionalnyi ahrarnyi universytet [Bila Tserkva National Agrarian University]. no. 2:155, pp. 60–74. Doi:10.33245/2310-4902-2020-160-2-158-165
  6. Tyshkivska, A.M., Dukhnytskyi, V.B. (2020). Vplyv preparativ polodoksynu ta tylmoksu 25 % na biokhimichni pokaznyky syrovatky krovi kurchat-broileriv [The effect of polodoxin and tilmox 25% on the biochemical parameters of the serum of broiler chickens]. Suchasne ptakhivnytstvo [Modern poultry farming]. no. 7–8. Doi:10.31548/poultry2020.07-08.007
  7. Mayahi, M., Gharibi, D., Ghadimipour, R., Talazadeh F. (2016). Isolation, identification and antimicrobial sensitivity of Ornithobacterium rhinotracheale in broilers chicken flocks of Khuzestan, Iran. Veterinary Research Forum. no. 7(4), pp. 341–346. PMID: 28144427
  8. Hauck, R., Chin, R.P., Shivaprasad, H. L. (2015). Retrospective study on the isolation of ornithobacterium rhinotracheale from chickens and turkeys in central california: 294 cases (2000-12). Avian Diseases. Vol. 59(1), pp. 130–137. Doi:10.1637/10935-091114-RegR
  9. Xiong, J., Zhu, Q., Yang, S. (2019). Comparison of pharmacokinetics of tilmicosin in healthy pigs and pigs experimentally infected with Actinobacillus pleuropneumoniae. New Zealand Vveterinary Journal. Vol. 67(5), pp. 257–263. Doi:10.1080/00480169.2019.1633434.
  10. Ludden, T.M. (1985). Pharmacokinetic interactions of the macrolide antibiotics. Clinical Pharmacokinetics. Vol. 10(1), pp. 63–79. Doi:10.2165/00003088-198510010-00003.
  11. Kilic, A., Timurkaan, N., Ertaş, A.A., Yilmaz, F. (2009). Pathological examination and bacterial re-isolation by culture and PCR of experimental Ornithobacterium rhinotracheale infection in broiler chickens. Revue de médecine vétérinaire. Vol. 160(3), pp. 140–144. Available at:https://
  12. Oca-Jimenez, R.M., Vega-Sanchez, V., Morales-Erasto, V. (2018). Phylogenetic relationship of Ornithobacterium rhinotracheale strains. The Journal of veterinary medical science. Vol. 80(6), pp. 869–873. Doi:10.1292/jvms.17-0474
  13. Ellakany, H.F., Elbestawy, A.R., Abd-Elhamid, H.S. (2019). Effect of experimental Ornithobacterium rhinotracheale infection along with live infectious bronchitis vaccination in broiler chickens. Poultry Science. Vol. 98(1), pp. 105–111. Doi:10.3382/ps/pey324
  14. Elsayed, M., Elkomy, A., Aboubakr, M., Morad, M. (2014). Tissue residues, hematological and biochemical effects of tilmicosin in broiler chicken. Veterinary Medicine International. Vol. 4. Doi:10.1155/2014/502872
  15. El-Mahmoudy, A., Gheith, I. (2016). The anti-nociceptive potential of tilmicosin against chemical-induced but not thermal-induced pain in mice. Internet Journal Immunopathology Pharmacology. Vol. 29(1), pp. 9–16. Doi: 10.1177/0394632015593232.
  16. Elkomy, A.A., Eltanany, N., Aboubakr, M. (2018). Pharmacokinetics and tissue residues of tilmicosin in normal and experimentally Mycoplasma gallisepticum infected broiler chickens. Benha Veterinary Medical Journal. Vol. 5(1), pp. 11–16. Doi:10.14419/ijpt.v5i1.7084
  17. Creemer, L., Beier, R., Kjehl, D. (2003). Facile synthesis of tilmicosin and tylosin related haptens for use as protein conjugates. The journal of antibiotics. Vol. 56(5), pp. 481–487. Doi:10.7164/antibiotics.56.481
  18. Rasheed, M., Ashraf, M., Javeed, A., Anjum, A.A. (2018). Toxicological evaluation of tilmicosin after intramuscular injection in broiler chicken. The Journal of Animal & and Plant Sciences. Vol. 28(6), pp. 1678–1686. ISSN: 1018-7081. Available at:https://www.thejaps.
  19. Modric, S., Martine, M. (2018). Patient variation in veterinary medicine-part II-influence of physiological variables. Journal of Veterinary Pharmacology aAnd Therapeutics. Vol. 34(3), pp. 209–223. Doi:10.1111/j.1365- 2885.2010.01249.x
  20. Levchenko, V.I., Novozhytska, Yu.M., Sakhniuk, V.V., Zhyla, I.A. (2004). Biokhimichni metody doslidzhennia krovi tvaryn. metodychni rekomendatsii. [Biochemical methods of animal blood research. Guidelines] 104 p.
PDF icon tyshkivska_a_1_2021.pdf650.14 KB