You are here

Nonspecifi c resistance of the rabbits organism in case of рassalurosis

Researching the characteristics of immunity is the best way for eff ective prevent and treat the diseases. Helminthiasis immunity has a number of features that are caused by relationships in the host-parasite system. Passalurosis is quantitatively dominant among many types of rabbit helminths on the globe. The goal of the work was to determine the infl uence of Passalurus ambiguuson indicators nonspecifi c resistance of the rabbits organism. Analog groups of male rabbits of 3-5 months old were selected for the experiments. Intensity of invasion was determined by the method of the Mac-Master.Rabbits with рassalurosis had diff erent levels of invasion intensity (II): low (II = 276.47 ± 43.33 eggs in 1 g of feces)–І, medium (II=1293.75 ± 275.80 eggs in 1 g of feces) – II and high (II= 2446.67 ± 422.11 eggs in 1 g of feces) – III research groups. We did not fi nd helminth’s eggs in the control group. It was found that in blood of sick animals with high II phagocytic activity is lower than in blood of healthy ones by 9.71% (p<0.01)A low indicator of phagocytic activity shows depressed phagocytosis in the organism of animals suff ering from рassalurosis of rabbits. In these rabbits, the phagocytic number was probably lower by 14.08% (p<0.01) compared to the control. Low levels of BASK and LASK were observed in infected animals by 5.45% (p<0.05), 8.00% (p<0.01), 14.49% (p<0.001) and 4.15% (p<0.001), 5.22% (p<0.001), 7.04% (p<0.001), respectively, in animals I , II and III groups than in the control. The decrease in the se in dicators in sick animals in dicates a weakening of the factors of non specifi c natural resistance of the organism. Analyzing the level of circulating immune complexes, we found a high level of medium and small CIC in clinically ill rabbits with low, medium and high II, respectively, 2.23 times (p <0.001), 2.37 times (p <0.001), 2.74 times (p <0.001) and 1.95 times (p<0.01), 2.09 times (p<0.001) and 2.22 times (p<0.001), against the control. An increase in the leve of CІC indicated the development of immunotoxicosis syndrome, the se verity of which correlated with the level of ІI of the disease.

Key words: phagocytic activity, bactericidal activity, lysozyme activity, Circulating immune complexes, рassalurosis, Passalurus ambiguus.

 

 

1. Boag, В. (1985). Helminth parasites from the wild rabbit orycto-laguscuniculus (L). Journal of Helminthology. Vol. 58, pp. 61–69.

2. Sonon, T. (1986). Enquetesur Pelevage du lapin dans la province du Mono. Memoire pour obtention du DETS, C.P.U., Abomey-calavi (Benin). pp. 123–128.

3. Sheng, L., Cui, P., Fang, S.-F., Lin, R.-Q., Zou, F.-C., &Zhu, X.-Q. (2014). Sequence variability in four mitochondrial genes among rabbit pinworm (Passalurus ambiguus) isolates from diff erent localities in China. Mitochondrial DNA. 2014. Vol. 26(4). Р. 501–504. Available at: https://doi.org/10.3109/19401736.2013.855898 

4. Mykhailiutenko, S.M., Kruchynenko, O.V., Klymenko, O.S., Serdioucov, J.K., Dmytrenko, N.I., &Tkachenko, V.V. (2019). Pathomorphological changes in the large intestine of rabbits parasitised by Passalurus ambiguus (Nematoda, Oxyuridae). Regulatory Mechanisms in Biosystems. Vol. 10, no. 1, pp.  69–74. Available at: https:// doi.org/10.15421/021911

5. Duda, Y. V., Kuneva, L.V., &Khrystyan, O.V. (2017). Pokaznyky bilkovogo obminu kroliv za pasaluroznoi' i'nvazii' [Indicators of protein metabolism of rabbits during pasalous invasion]. Naukovo-tehnichnyj bjulleten' Naukovo-doslidnogo centru biobezpeky ta ekologichnogo kontrolju resursiv APK [Science and Technology Bulletin of the APC Biosafety Research and Environmental Control Center]. Vol. 5, no. 1, pp. 93–96.

6. Liu, G.H., Li, S., Zou, F.C., Wang, C.R., Zhu, X.Q. (2015). The complete mitochondrial genome of rabbit pinworm Passalurus ambiguus: genome characterization and phylogenetic analysis. Parasitology Research. Vol. 115(1), pp. 423–429. Available at: https://doi.org/10.1007/s00436015-4778-3

7. Abdel-Gaber, R., Ataya, F., Fouad, D., Daoud, M., &Alzuhairy, S. (2019). Prevalence, Morphological and Molecular Phylogenetic Analyses of the Rabbit Pinworm, Passalurus ambiguous Rudolphi 1819, in the Domestic Rabbits Oryctolaguscuniculus. Acta Parasitologica. Vol. 64(2), pp. 316–330. Available at: https://doi.org/10.2478/ s11686-019-00047-7

8. Dubnitsky, A.A. (1974). Passaluroz [Passalurosis]. Bolezni krolikov [Diseases of rabbits]. Moscow: Colossus, pp. 184–190.

9. Florian, D.D. (1997). Passaluroz krolikov v uslovijah Moskovskoj oblasti (biologija vozbuditelja, jepizootologija i mery bor'by): avtoref. dis. … kand. vet. nauk. [Passalurosis of rabbits in the Moscow region (pathology biology, epizootology and control measures): abstract of a dissertation of a candidate of veterinary sciences]. Moscow, 22 p.

10. Kornienko, L.E., Dombrovsky, O.B., Ponomar, S.I., Antipov, A.A. (2003). Infekcijni ta invazijni hvoroby kroliv [Infectious and invasive diseases of rabbits]. BilaTserkva, 288 p.

11. Grishina, E.A., Dovgalev, A.S. (2016). Nekotorye mehanizmy vtorichnoj immunosupressii v processe hronizacii geogel'mintozov [Some mechanisms of secondary immunosuppression in the process of chronic geohelminthiasis]. Rossijskij parazitologicheskij zhurnal [Russian parasitological journal].  no. 2(36),  pp. 202–209.

12. Duda, Y.V. (2019) Klitynnyj imunitet kroliv za vplyvu Treponema cuniculi [Cellular immunity of rabbits by Treponema cuniculi]. Naukovotehnichnyj bjuleten' DNDKI veterynarnyh preparativ ta kormovyh dobavok i Instytutu biologii' tvaryn NAAN [Scientifi c and Technical Bulletin of the State Scientifi c Research Institute of Veterinary Drugs and Feed Additives and the Institute of Animal Biology of NAAS]. Issue 20, no. 2, pp. 223–229. Available at: https:// doi.org/10.36359/ scivp.2019-20-2.28

13. Grishina, E.A. (2016). Antigeny i metabolity gel'mintov kak regulirujushhie faktory protivoparazitarnogo immuniteta [Helminth antigens and metabolites as regulatory factors of antiparasitic immunity]. Jepidemiologija i infekcionnye bolezni [Epidemiology and infectious diseases]. Aktual'nye voprosy [Actual issues]. no. 2, pp. 58–63.

14. Duda, Y.V. (2019). Nespecyfi chna reaktyvnist' organizmu kroliv za vplyvu cystycerkoznoi' invazii' [Nonspecifi c reactivity of the rabbit organism under the infl uence of cysticercosis invasion]. Naukovyj visnyk LNUVM ta BT imeni S. Z. G'zhyc'kogo. [Scientifi c Bulletin of the LNUVM and BT named after S. Z. Zhytsky]. Vol. 21(94),  pp. 132–135. Available at: https://doi.org/10.32718/ nvlvet9424 

15. Boroda, N.A., Vishchur, O.I., Ratskyi, M.I. (2011). Pryrodna rezystentnist' organizmu koriv ta i'h teljat za dii' preparatu "Oligovit" [The natural resistance of the body of cows and their calves under the action of the drug "Oligovit"]. Biolohiia tvaryn [Animal biology]. Vol. 13(1–2),  pp. 397–401.

16. Tatarchuk, L.V. (2018). Osoblyvosti miscevyh imunnyh reakcij u porozhnij kyshci pry postrezekcijnij portal'nij gipertenzii' [Features of local immune responses in the empty gut in postresection portal hypertension]. Shpytal'na Hirurgija [Hospital Surgery]. Zhurnal Imeni L. Ja. Koval'chuka [The name of L.Y. Kovalchuk]. no. (2). Available at: https://doi.org/10.11603/2414-4533.2018.2.9201

17. Lazareva, Y.B., Filippova, A.V., Romanova, L.M., Grishina, E.A. (2010). Aktual'nye problemy podavlenyja ymmunyteta pry gel'mintozah [Actual problems of suppression of immunity in helmintosis]. Fundamental'nye nauky y praktyka [Fundamental sciences and practice]. Sbornyk nauchnyh trudov [Collection of scientifi c papers].  Issue 2, pp. 70–71.

18. Wood, I.B., Amaral, N.K., Bairden, K. (1995). World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) second edition of guidelines for evaluating the effi  cacy of anthelmintics in ruminants (bovine, ovine,caprine). Vet Parasitol. no. 58, pp. 181–213. Available at: https://doi.org/10.1016//0304-4017(95)00806-2

19. Vlizlo, V.V., Fedorchuk, R.S., Ratych, I.B. (2012). Laboratorni metody doslidzhen' u biologii', tvarynnyctvi ta veterynarnij medycyni [Laboratory research methods in biology, animal husbandry and veterinary medicine]. Lviv: Spolom, 764 p.

20. Smirnova, O.V., Kuzmina, T.A. (1966). Opredelenye bakterycydnoj aktyvnosty syvorotky krovy metodom fotonefelometryy [Determination of bactericidal activity of blood serum by photonephelometry]. ZhMJeI [LMIC].  no. 4, pp. 8–11.

21. Dorofejchuk, V.G. (1968). Lizocimnaja aktivnost' syvorotki krovi [Lysozyme activity of  blood serum]. Laboratornoe delo [Laboratory work]. no. 1, pp. 28–34.

22. Shuturma, O.J., Lіsnichuk, N.Е., Dovgaljuk, A. І. (2013). CIK ta i'h rol' u vynyknenni pojednanyh patologij organiv travnoi' systemy [The CEC and their role in the emergence of joint pathologies of the digestive system]. Zdobutky klinichnoi' i eksperymental'noi' medycyny [Achievements of clinical and experimental medicine]. no. 2, pp. 223–225.

23. Shimazaki, Y., Takahashi, A. (2018). Antibacterial activity of lysozyme-binding proteins from chicken egg white. Journal of Microbiological Methods. Vol. 154. pp. 19–24. Available at: https://doi.org/10.1016/j.mimet.2018.10.001

24. Subbaraman, L., McCanna, D., Oh, S., Ng, A., Coles-Brennan, C., Fadli, Z., Jones, L. (2018). Lysozyme activity on contact lenses and the impact of denatured lysozyme on human corneal epithelial cells. Contact Lens and Anterior Eye. Vol. 41. Р. 20–S21. Available at: https:// doi.org/10.1016/j.clae.2018.04.145

25. Dumych, T., Paryzhak, S., Bilyy, R. (2019). Involvement of neutrophil hydrolytic enzymes in the modifi cation of circulating immune complexes under the circumstances of experimental sepsis. Proceedings of the Shevchenko Scientifi c Society. Medical Sciences. Vol. 55(1), pp. 31–39. Available at: https://doi.org/10.25040/ntsh2019.01.03

AttachmentSize
PDF icon duda_2_2019.pdf2.41 MB