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Peculiarities of acute phase reaction and its correction in surgical pathology in pigs

Surgical pathology in animals is quite broad and has various forms of manifestation. It covers various anatomic-topographic areas and organs, in particular in pigs quite often presented with abdominal pathology. There have been published reports of an incidence of hernias in 0.8-3.5% of the pig population, which is 50% of all surgical pathology of the whole animal species.

In the group of clinically healthy pigs (n = 10), a significant level of anti-inflammatory IL-10 is noticeable. At the same time, rather high cytokine indices of the ratio of anti-inflammatory to proinflammatory cytokines in healthy animals – 13.9:
1 – 19.4: 1, with the index between the latter – 0.7: 1. That is, in general, IL-10, under physiological norms, exercises a powerful control over the phylogenic cytokine systems of the pig body.

At the same time, animals with surgical pathology show a sharp increase in the levels of proinflammatory cytokines. For hernias accompanied by an adhesive-inflammatory aseptic process, serum TNF-α content is increased 4.9-fold and IL-1β is slightly less – only 2.1-fold, with a moderate increase in the concentration of 1.3-fold anti-inflammatory IL- 10. In this case, cytokine indices are significantly reduced, which indicates a decrease in the activity of antiflogogenic mechanisms.

The dynamics of the development of purulent arthritis is characterized by a remarkable increase in blood levels of
TNF-α – 18.3 times, and a moderate increase in the concentrations of IL-1 and IL-10. In this case, the cytokine index of
IL-10: TNF-α acquired a critical value of 1.5: 1, and the number of the latter exceeded the level of IL-1β by more than
5 times. At the same time, elevation of IL-1β and IL-10 levels did not lead to a significant difference with the indigenous animals. Most likely, the powerful synthesis of TNF-α is associated with the appearance of a large number of cells of its producers in the area of ​​destruction of the joints. In this case, insufficient increase in the level of IL-10 triggers the process of cascading production of inflammatory mediators and the development of systemic inflammatory syndrome.

Fibrinogen, as one of the acute phase reaction proteins, fluctuates within 2–4 g / L by physiological norms. In the early postoperative period, its level increases significantly, however, the peaks of increasing plasma fibrinogen concentration in control animals are recorded on day 1 and day 7. By using the immune depot, on the contrary, it is dynamically normalized to the 7th day after the hernia. The dynamics of fibrinogen concentration with the use of thiotriazoline were somewhat different. It peaked after the first day and was 2.3 times higher than immuno depot injections. In the following, only after the 3rd day the level of fibrinogenemia began to normalize dynamically, which can be explained by the pronounced hepatoprotective effect of thiotriazoline with increased synthesis of fibrinogen by the liver.

The level of haptoglobin, like fibrinogen, remained virtually unchanged in the first hours after herniotomy. However, its concentration increased substantially 24 hours after surgery in the first and second study groups, compared to the preoperative period. Moreover, the level of haptoglobin in the 1st group was 2 times higher than in the 2nd (p <0.01), which indicates a more pronounced anti-inflammatory effect of thiotriazoline. On the 3rd day of the postoperative period there was a peak increase of this indicator, but again in the 2nd experimental group it was twice lower. A further dynamic decrease in serum haptoglobin concentration was more pronounced with the use of thiotriazoline. With regard to ceruloplasmin, it reached its highest level by the 6th hour after surgical intervention in pigs of group 1 and amounted to 421.9 ± 39.69 mg / l. The rather rapid increase in serum ceruloplasmin is due, most likely, to the inhibition of prooxidant enzymes by the components of the immune depot, in particular selenium and copper. However, given the established dynamics of the concentration of ceruloplasmin in groups, it is not possible to attest to its specificity for aseptic inflammation in pigs.

 The cytokine profile in pigs with surgical pathology depends on its complexity. Moderate cytokinemia of phlogenic character develops over hernia, which becomes extremely pronounced with purulent arthritis. However, the production of anti-inflammatory cytokines is not adequate enough to require pharmacological correction of the inflammatory response in surgical pathology in pigs. The acute-phase reaction after herniotomy in pigs is mainly manifested by an increase in the concentration of fibrinogen and haptoglobin in the blood, but not ceruloplasmin. The mechanisms of its correction with thiotriazoline and immune depot differ, which needs further research.

Key words: pigs, cytokines, acute phase proteins, herniotomy, immune depot, thioriazoline.

1. Alekseeva, I.V. (2006). Novye razrabotki dlja lechenija zhivotnyh pri gnojno-vospalitel'nyh processah [New developments for the treatment of animals with purulent-inflammatory processes]. Veterinarija [Veterinary Medicine].  no. 5, pp. 52−56.

2. Hanjejev, V.V. (2004). Gemostaz ta jogo korekcija pry hirurgichnij infekcii' u sobak:  avtoref. dys.  kand. vet. nauk: 16.00.05. [Hemostasis and its correction in surgical infection in dogs: abstract of dissertation of the candidate of veterinary sciences: 16.00.05.]. Bila Tserkva, 23 p.

3. Mastyko, G.S. (1985). Asepticheskie i septicheskie vospalenija u s.–h. zhivotnyh [Aseptic and septic inflammations in the village animals]. Minsk: Harvest, 40 p.

4. Rublenko M.V., Іl'nіc'kij, M.G. (1995). Rozpovsjudzhennja hіrurgіchnoї patologії u svinej pri utrimannі na rіznih pіdlogah [Dissemination of surgical pathology in pigs when kept on different floors]. Neіnfekcіjna patologіja tvarin : nauk.-prakt. konf., 7−8 chervnja 1995 r.: tezi dop. [Non-communicable animal pathology: scientific-practical. Conf., June 7-8, 1995: these suppl.]. Bila Tserkva,  pp. 188−190.

5. Tykhoniuk, L.A., Nahornyi, V.V., Chornozub, M.P. (2006). Zastosuvannia odnopoverkhovoho visimkopodibnoho shva dlia zakryttia hryzhovoho kiltsia pry herniotomii u porosiat [The use of single-storey visimkopodibnoho suture to close the hernia ring at herniotomiyi in pigs]. Visnyk Bilocerkiv. derzh. agrarn. un-tu: zb. nauk. prac'.  [Bulletin of the Bilotserkiv State Agrarian University: a collection of scientific papers]. Vol. 41, pp. 246–250.

6. Maslikov, S. (2007). Likuvannya vpravnih grizh u svijskih tvarin [Treatment of skilled hernias in domestic animals]. Vet. medicina Ukrayini [Vet. medicine of Ukraine]. no.10. pp. 18–19.

7. Shnjakina, T.N., Shherbakov, N.P. (2004). Gryzhesechenie pahovo-moshonochnyh gryzh u hrjachkov s ostavleniem semennika v polosti moshonki [Hernia repair of inguinal-scrotal hernias in boars, leaving the testis in the scrotum cavity]. Aktual'nye problemy veterinarnoj mediciny, posvjashh. 75-letiju UGAVM : mezhdunar. nauch.-prakt. konf. : tezisy dokl. [Actual problems of veterinary medicine, dedicated. 75th anniversary of the UGAVM: int. scientific-practical Conf .: abstracts ]. Troitsk, pp. 177–179.

8. Rublenko, M.V. (2000). Patogenetychni osoblyvosti zapal'noi' reakcii' u svynej pry hirurgichnyh hvorobah ta metody i'h likuvannja : avtoref. dys. … dokt. vet. nauk: spec. 16.00.05 [Pathogenetic features of inflammatory response in pigs in surgical diseases and methods of their treatment: author's abstract the dissertation of Doctor of Veterinary Sciences: specialization 16.00.05.]. Bila Tserkva, 36 p.

9. Andrijec', V.G. (2009). Sudynno-trombocytarnyj gemostaz ta jogo korekcija pry abdominal'nij hirurgichnij patologii' u sobak i svynej: dys. … kand. vet. nauk: spec. 16.00.05 [Vascular-platelet hemostasis and its correction in abdominal surgical pathology in dogs and pigs: dissertation of the candidate of veterinary sciences: specialization 16.00.05.]. Bila Tserkva, 33 p.

10. Shaganenko, V.S. (2012). Kliniko-patogenetychna rol' oksydu azotu ta korekcija jogo rivnja za hirurgichnoi' patologii' zapal'nogo g'enezu v tvaryn riznyh vydiv: avtoref. dys. … kand. vet. nauk: spec. 16.00.05. [Clinico-pathogenetic role of nitric oxide and correction of its level in surgical pathology of inflammatory genesis in animals of different species: abstract the dissertation of the candidate of veterinary sciences: specialization 16.00.05.]. Bila Tserkva, 23 p.

11. Hobbs, A. (2005). Nitric oxide metabolism linked to sepsis. J. Biol. Chemistry.  Vol. 18, no. 3, pp. 721–729.

12. Fukumura, D., Kashiwagi, S., Jain, R. (2006). The role of nitric oxide in tumour progression. Nat. Rev. Cancer. Vol. 6, pp. 521–534.

13. Ying, L., Hofseth, L. (2007). An emerging role for endothelial nitric oxide synthase in chronic inflammation and cancer. Cancer Res. Vol. 67, pp. 1407–1410.

14. Hussain, S., Subleski, J., Hofseth, L. (2008). Nitric oxide is a key component in inflammation-accelerated tumorigenesis. Cancer Res.  Vol. 68, pp. 7130–7136.

15. Belicer, V.O., Varec'ka, T.V., Veremjejenko, K.M. (1997). Kil'kisne vyznachennja fibrynogenu v plazmi krovi ljudyny [Quantitative determination of fibrinogen in human blood plasma.].  Labor. Diagnostyka [Laboratory diagnostics]. no. 2, pp. 53–55.

16. Kamyshnikov, V.S. (2004). Spravochnik po kliniko-biohimicheskim issledovanijam i laboratornoj diagnostike [Handbook of clinical and biochemical studies and laboratory diagnostics]. Moscow "Medpress-inform".

17. Opal, S.M.,  DePalo, V.A. (2000). Anti-inflammatory cytokines, Chest 111. pp. 1162–1172.

18. Bradley, J.R. (2008). TNF-mediated inflammatory disease. J Pathol. Vol. 214, рр. 149–160.

19. Christodoulou, C., Choy, E.H. (2006). Joint inflammation and cytokine inhibition in rheumatoid arthritis. Clin Exp Med. Vol. 6, pp. 13–19.

20. Larmonier, C.B., Laubitz, D., Thurston, R.D. (2011). NHE3 modu­lates the severity of colitis in IL-10-deficient mice. Am J Physiol Gastrointest Liver Physiol. 300, pp. 998–1009.

21. Cao, W., Zhang, W., Liu, J. (2011). Paeoniflorin improves survival in LPS-challenged mice through the suppression of TNF-alpha and IL-1beta release and augmentation of IL-10 production. Int Immunopharmacol. Vol. 11, pp. 172–178.

22. Rublenko, M.V., Vlasenko, S.A., Andrijec', V.G., Jeroshenko, O.V., Mel'nikov, V.V. (2012). Cytokinovyj status, reakcija gostroi' fazy ta i'i' korekcija za akushers'koi' ta hirurgichnoi' patologii' u produktyvnyh ta dribnyh domashnih tvaryn [Cytokine status, acute phase response and its correction in obstetric and surgical pathology in productive and small animals]. Bila Tserkva, 21 p.

23. Nathan, C. (2002). Points of control in inflammation. Nature. Vol. 420, 846 p.

24. Caruso, R., Warner, N., Inohara, N., Nunez, G. (2014). NOD1 and NOD2: signaling, host defense, and inflammatory disease. Immunity. Vol. 41, pp. 898–908.

25. Piccinini, A.M., Midwood, K.S. DAMPening inflammation by mod-ulating TLR signalling. Mediat Inflamm, 2010.

26. Henry, G., Garner, W. (2003). Inflammatory mediators in wound healing. Surg. Clin. North Am. Vol. 83, 483 p.

27. Rublenko, M.V., Mel'nikov, V.V. (2012). Cytokinovyj status svynej z hirurgichnoju patologijeju [Cytokine status of pigs with surgical pathology]. Х mizhnarodnyj kongres specialistiv veterynarnoi' medycyny: materialy kongresu [X International Congress of Veterinary Specialists: Congress materials]. pp. 153–154.

28. Rublenko, M.V, Mel'nіkov, V.V. (2012). Osoblivostі lejkocitarnoуі reakcіуі u svinej pіslja grizhoroztinu ta za umov korekcіуі zapal'no-regenerativnogo procesu іmunostimuljatorami rіznih grup [Peculiarities of leukocyte reaction in pigs after herniation and under the conditions of correction of inflammatory-regenerative process by immunostimulators of different groups]. Naukovo-teoretichnij zhurnal Bіologіja tvarin – L'vіv [Theoretical Journal of Animal Biology – Lviv]. Vol. 14, no.1–2, pp. 557–562.

29. Andrijec', V.G., Mel'nikov, V.V. (2010). Vmist u plazmi krovi koriv z ortopedychnoju patologijeju nyzky gostrofaznyh bilkiv [Content in the blood plasma of cows with orthopedic pathology of a number of acute-phase proteins]. Naukovyj visnyk veterynarnoi' medycyny: zb. nauk prac' [Scientific Bulletin of Veterinary Medicine: Collection of Scientific Papers]. Bila Tserkva, Issue 4(67), 172 p.

30. Petersen H.H., Nielsen J.P., Heegaard P.M. Application of acute phase protein measurements in veterinary clinical chemistry. Vet. Res. 2004. Vol. 35. pp. 163–187.

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