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Quail ovarian tissue Superoxide dismutase and catalase under the influence of a complex of essential amino acids in combination with vitamin E
Intramolecular oxidation of biological substrates (biological oxidation) is the main molecular mechanism by which ener-
gy needs are provided with the functioning of living organisms. The intensity of lipid peroxidation is conditioned by free
radical chemicals that occur as a result of metabolism. Intensification of lipid peroxidation contribute to reducing the intracel-
lular content of antioxidants such as tocopherol, retinol, glutathione, selenium and other.
By enzyme systems of biological membranes from damage due to lipid peroxidation include enzymes catalase and su-
peroxide dismutase. Superoxide dismutase is an enzyme that protects the body from toxic products that are constantly gener-
ated during metabolism. Under the influence of this enzyme superoxide radicals are converted to less active oxidants – hy-
drogen peroxide and oxygen.The role of catalase is to prevent the accumulation of hydrogen peroxide, which is formed by
dismutation of superoxide anion in anaerobic oxidation flavoproteyidiv restored. As a result of such reactions produced water
and molecular oxygen, which are used for further physiological needs of the body. One of the natural antioxidants are vita-
min E, its deficiency in the diets of animals leads to changes in ultrastructure of cell membranes and enhance the destructive
effects of free radicals on cell membranes and organelles. Methionine is precursor all sulfur compounds in the body and a
source of sulfur in detoxification processes. Methionine (methyl groups) can participate in the synthesis of polyamines (prop-
ylamine), which play an important role in the antioxidant protection of tissues. In addition, it was found that methionine can
be used to build other sulfur-containing amino acids – cysteine, which is also part of glutathione. Glutathione is actively in-
volved in redox processes, protecting SH-groups of enzymes and other proteins from oxidation, restoring H 2 O 2 and ensuring
transport of amino acids across the membrane of cells. According to with cysteine also can form other biologically active
substances, such as acetylcysteine, which has antioxidant, anti-toxic and immune-modulating properties.
Prior to the experiment superoxidedismutase activity in ovarian tissue of quails all groups was within 28,3–31,6 mind.
units./mg. On the 15th day of the experiment the tendency to increase the activity of this enzyme in the experimental groups. In par-
ticular, in group 2, the growth was 14,8 % and in the 3rd, activity was greater than in the control to 10,4 %, while in the 4th group of
the enzyme activity remained unchanged. On the 30th day of research activity superoxidedismutase of in the 2nd and 3rd group was
significantly higher compared with control 20,3 and 14,4 %, respectively, and in group 4 activity increase was only 8,0 % compared
with the control, that it was not likely. On the 45th day of the experiment likely increase in SOD activity was observed in all experi-
mental groups and accounted for 12,1, 10,1 and 14,2 %. If we trace the dynamics of changes in superoxidedismutase activity in the
ovaries poultry 2nd and 3rd group, growth observed during the experiment, which in our opinion could indicate a decrease in the
concentration of H 2 O 2 and lipid peroxidation products in these organs. Investigational enzyme activity gradually increased with
increasing intensity oviposition. We can assume that the increased activity of SOD is a reflex response to the formation of lipid pe-
roxidation products gain since the beginning of oviposition to maximize it. Enrichment of dietary quail study groups during the ex-
periment vitamin E causes modulation of antioxidant enzymes and led to the activation lipid peroxidation , as evidenced by the
growth of this indicator especially in poultry in 2nd and 3rd group compared with the control. The highest growth among the re-
search groups of birds were in group 2, which shows in our opinion, the strengthening of lipid peroxidation during oviposition,
which was the largest in the group, as well as a good supply of vitamin E diet of quail. There are anecdotal reports that bird re-
sistance lipid peroxidation accompanied by decreased activity, however, in our experiment found that during the experiment super-
oxidedismutase activity increased gradually as quails in research and in the control group. The only difference is that in bird research
groups who received dietary supplements essential amino acids and vitamin E, growth was more significant. Thus, the addition to
the diet of a certain amount of vitamin E contributes superoxidedismutase activity compared to control during the whole period of
research. You can suggest that vitamin E interacting with residual oxygen neutralizes it, thereby increasing the body's defense
against lipid peroxidation products. catalase activity to some extent related to the activity of superoxidedismutase and during ontoge-
netic development of animals.
The experiment catalase activity in ovarian tissue quails all groups ranged from 10.88 to 12.30 mmol/min×mg. Since the
beginning of oviposition, the 15th day, significantly increased catalase activity of quail in the control group by 11.8 %, while
the experimental groups on average 14,6–28,6 %. On the 30th day of the experiment, the activity of catalase in the second
group was significantly higher compared to the control group to 26,3 % in the 3-th and 4-th group it grew by 18,8–11,5 %,
that is increased activity was not likely. On the 45th day of the experiment catalase activity in the second and third experi-
mental groups significantly increased to 23,74±1,22-22,14±1,33 mmol/min×mg or was greater than the control at 31,9–23,0 %
respectively. In the fourth group of catalase activity was 21,18±1,99 mmol/min×mg and was higher compared with the con-
trol 17,7 %, but this increase was not likely. Analyzing changes in ovarian tissue catalase activity during the experiment
should note its growth in all groups of birds. In our view, these changes reflect the catalase activity increased metabolism in
the body hens, which explains the beginning of oviposition. Such changes catalase activity is adaptive reaction laying hens to
increase the intensity of metabolic processes that ensure strengthening oogenesis in quails, and increased flow of nutrients to
the ovaries quails. With the emergence of puberty and the start of oviposition investigational enzyme activity in all groups of
quails grew gradually, and dietary supplements quails amino acids and vitamin E had a positive impact on the activity of
catalase and course lipid peroxidation. The diet complex essential amino acids combined with vitamin E contributed to the
significant increase of superoxide dismutase and catalase activity in ovarian tissue quails of experimental group compared
with the control. Changes enzyme activity reflects activation of metabolism in hens research groups and enhancing lipid pe-
roxidation associated with the start of oviposition.
Key words: quail, lysine, methionine, threonine, vitamin E, antioxidant protection, superoxide dismutase, catalase.
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