You are here
The separation and detection of several mycotoxins by thin-layer chromatography A. Bilan
The article described metods alternatively, T-2 toxin will afford a bright blue fluorescence if treated with a mixture of
aluminium chloride (in water:ethanol, 1:1) and chromotropic acid (in concentrated sulphuric acid:water, 5:3) followed
by heating at 11 0 °C. The natural fluorescence of sterigmatocystin may be enhanced, to afford a bright yellow spot, by
spraying with a 24 percent solution of aluminium chloride in 95 percent aqueous ethanol and heating at 105 °C for
10 minutes. The identity of sterigmatocystin may be confirmed by the formation of the acetate or hemiacetal derivatives. Similarly,
the long-wave fluorescence of zearalenone and ochratoxin A may be enhanced if the plate is sprayed with aluminium chloride solu-
tion. The presence of ochratoxin A may be confirmed by the formation of the ethyl ester derivative.
Key words: mycotoxins, extracts, cleaning, thin layer chromatography, detection.
1. Forgacs J. Feedstuff / J. Forgacs // Appl. Microbiol – 2004. – Vol.34. – P. 124.
2. Nature / K.Sargeant, A.Sheridan, J.O'Kelly [et al.] // Med. Pregl. – 2009. – Vol. 192. – P. 1095.
3. American Academy of Pediatrics. Toxic effects of indoor molds. Pediatrics. – 2006. – Vol.101. – P. 712-714.
4. Anderson S. J. Compositional changes in surface mycoflora during ripening of naturally fermented sausages /
S. J. Anderson // J. Food Protect. – 2005. – Vol.58. – P. 426-429.
5. Barrett J. Mycotoxins: of molds and maladies / J. Barrett // Environ. Health Perspect. – 2009. – Vol.108. – P. A20-A23.
6. Ochratoxin production by the Aspergillus ochraceus group and Aspergillus alliaceus. / P. Bayman, J. L. Baker, M. A.
Doster [et al.] // Appl. Environ. Microbiol. – 2002. – Vol.68. – P. 2326-2329.
7. Beardall J. M. Disease in humans with mycotoxins as possible causes. / J. M. Beardall, J. D. Miller. – 2004. –
P. 487-539.
8. Beasley V. R. Trichothecene mycotoxicosis: pathophysiologic effects / V. R. Beasley. – 2009. – Vol.1. – P. 10.
9. Belkin L. Haunted by mold / L. Belkin // New York Times Magazine. – 2001. – Vol.62. – P. 64-65.
10. Bennett J. W. Mycotoxins, mycotoxicoses, mycotoxicology and mycopathology / J.W. Bennett // Mycopathlogia. –
2007. – Vol.100. – P.3-5.
11. Bennett J. W. Pride and prejudice: the story of ergot / J. W. Bennett, R. Bentley // Perspect. Biol. Med. – 1999. –
Vol.42. – P. 333-355.
12. Bennett J. W. One gene to whole pathway: the role of norsolorinic acid in aflatoxin research. / J. W. Bennett, P.-K.
Chang and D. Bhatnagar // Adv. Appl. Microbiol. – 2007. – Vol.45. – P. 1-15.
13. Bentley R. Constructing polyketides: from Collie to combinatorial biosynthesis. / R. Bentley and J. W. Bennett
// Annu. Rev. Microbiol. – 1999. – Vol.53. – P. 411-446.
14. Berry C. L. The pathology of mycotoxins / C. L. Berry // J. Pathol. – 2008. – Vol. 154. – P.301-311.
15. Betina V. Mycotoxins: chemical, biological and environmental aspects / V. Betina. – 2009. – Vol.9. – P. 9.
16. Betina V. (ed.). 2004. Mycotoxins: production, isolation, separation, and purification / V. Betina // Elsevier,
Amsterdam, The Netherlands.
17. Blanc P. J. Production of citrinin by various species of Monascus / P.J. Blanc, M.O. Loret, G. Goma // Biotechnol.
Lett. – 2005. – Vol.17. – P. 291-294.