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Головна » Scientific Journal of Veterinary Medicine 1'2026

The role of pathogenic microorganisms in various mastitis forms development in cows

Mastitis in dairy cows is one of the most widespread and economically signifi cant diseases in modern dairy farming. It leads to decreased milk yield, deterioration of milk quality, increased culling rates, and additional treatment costs. Identification of causative agents is essential for effective therapy and prevention. The aim of this study was to determine the species composition and frequency of pathogenic microorganisms associated with different clinical forms of mastitis in cows. A total of 133 cows with clinical signs of mastitis were examined, and 346 milk samples from affected udder quarters were analyzed. The clinical distribution of mastitis cases was as follows: serous – 82,55 %, catarrhal – 6,98 %, fibrinous – 2,33 %, purulent – 2,33 %, and hemorrhagic – 5,81 %. Bacteriological examination revealed that serous mastitis was most often associated with Streptococcus uberis (4,22 %), Escherichia coli (2,82 %), and Pseudomonas aeru ginosa (2,82 %). In catarrhal cases, Streptococcus dysagalactiae (25,00 %), Staphylococcus aureus (20,83 %), and Streptococcus agalactiae (12,50 %) were prevalent. Fibrinous mastitis was characterized by infections with Staphylococcus aureus (37,50 %), Enterobacter spp. (50,00 %), and Pseudomonas aeruginosa (100 %). Purulent mastitis was mainly caused by Staphylococcus aureus (50,00 %) and Streptococcus uberis (37,50 %), while hemorrhagic mastitis had the most diverse microbial spectrum, including Escherichia coli (20,00 %) and Staphylococcus aureus (10,00 %). The results indicate the polyetiological nature of bovine mastitis. Streptococci are dominant in mild forms, whereas severe cases are more often associated with opportunistic Gram-negative pathogens. These fi ndings highlight the importance of routine microbiological monitoring, application of advanced diagnostic methods, and implementation of a complex approach to treatment and prevention of mastitis in dairy herds.

Keywords: mastitis in cows, mastitis pathogens, Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli, bacteriological culture, antibiotic therapy, antibiotic resistance, mastitis prevention.

CHEMEROVSKA I. https://orcid.org/0000-0002-7291-6400


RUBLENKO I. https://orcid.org/0000-0002-1401-0969


ZOTSENKO V. https://orcid.org/0000-0001-8908-6688


TITARENKO O. https://orcid.org/0000-0002-7370-8523


  1. Rainard, P. (2017). Mammary microbiota of dairy ruminants: fact or fiction? Veterinary Research, Vol. 48, no. 1, 25 p. DOI:10.1186/s13567017-0429-2.
  2. Al-Farha, A.B., Hemmatzadeh, F., Khazandi, M. (2017). Evaluation of effects of Mycoplasma mastitis on milk composition in dairy cattle from South Australia. BMC Veterinary Research, Vol. 13, 351 p. DOI:10.1186/s12917-017-1274-2.
  3. Reinoso, E.B. (2017). Bovine mastitis caused by Streptococcus uberis: virulence factors and biofilm. Journal of Microbial&Biochemical Technology, Vol. 9 (5), pp. 237–243. DOI:10.4172/19485948.1000371.
  4. Keefe, G.P. (2018). Update on control of Staph ylococcus aureus and Streptococcus agalactiae mastitis in dairy herds. Vet. Clin. North Am. Food Anim. Pract., Vol. 34 (3), pp. 491–505. DOI:10.1016/j.cvfa.2018.07.002.
  5. Srednik, M.E., Raspanti, C.G., Andreotti, C.S. (2018). Antimicrobial resistance and virulence factors in Staphylococcus aureus isolates from bovine mastitis in Argentina. Microbial Pathogenesis, Vol. 124, pp. 97–104. DOI:10.1016/j.micpath.2018.08.021.
  6. Zadoks, R.N., Middleton, J.R., McDougall, S. (2018). Molecular epidemiology of mastitis pathogens and the role of genomics. Veterinary Microbiology, Vol. 214, pp. 84–91. DOI:10.1016/j.vetmic.2017.12.031.
  7. Tomazi, T., Gonçalves, J.L., Nascimento, C.S. (2018). Identification of coagulase-negative staphylococci from bovine mastitis using MALDI-TOF MS. J. Dairy Sci., Vol. 101 (3), pp. 2925–2934. DOI:10.3168/jds.2017-13890.
  8. Ruegg, P.L. (2017). A 100-year review: mastitis detection, management, and prevention. J. Dairy Sci., Vol. 100 (12), pp. 10381–10397. DOI:10.3168/jds.2017-13023.
  9. Vakkamäki, J., Taponen, S., Koort, J., Pyörälä, S. (2017). Bovine mastitis in Finland: etiology and treatment outcomes. Acta Vet. Scand., Vol. 59, 60 p. DOI:10.1186/s13028-017-0323-4.
  10. Hogeveen, H., Huijps, K., Lam, T. (2019). Economic aspects of mastitis: new developments. NZ Vet. J., Vol. 67 (6), pp. 332–340. DOI:10.1080/00480169.2019.1656596.
  11. Feng, Y., Huang, X., Shi, C. (2020). Prevalence and antimicrobial resistance of major pathogens from bovine mastitis in China. Front. Vet. Sci., Vol. 7, 418. DOI:10.3389/fvets.2020. 00418.
  12. Wente, N., Zoche-Golob, V., Vries, M.De. (2019). Association between pathogens and clinical forms of mastitis. Vet. Microbiol., Vol. 235, pp. 79 87. DOI:10.1016/j.vetmic.2019.06. 002.
  13. Ashraf, A., Imran, M. (2018). Diagnosis of bovine mastitis: from laboratory to farm. Trop. Anim. Health Prod., Vol. 50 (6), pp. 1193–1202. DOI:10.1007/s11250-018-1552-8.
  14. Kaczorek-Łukowska, E., Małaczewska, J. (2021). Biofilm formation and antimicrobial resistance of S. aureus and S. uberis isolates from bovine mastitis. Animals (Basel), Vol. 11 (4), 170 p. DOI:10.3390/ani11061704.
  15. Supré, K., Haesebrouck, F. (2020). Importance of coagulase-negative staphylococci in bovine mastitis. Pathogens, Vol. 9 (1), 39 p. DOI:10.3390/pathogens9010039.
  16. Morales-Anaya, J., Krömker, V. (2021). Molecular mechanisms of mastitis pathogenesis in dairy cows. Microorganisms, Vol. 9 (5), 1099 p. DOI:10.3390/microorganisms9051099.
  17. Yang, F., Li, X., Li, M. (2019). Antimicrobial resistance and biofi lm formation of E. coli isolated from bovine mastitis. Front. Microbiol, Vol. 10, 208 p. DOI:10.3389/fmicb.2019.00208.
  18. Dordet-Frisoni, E., Pasquali, P., Guérin-Fauthoux, E. (2018). Whole-genome sequencing of S. aureus from bovine mastitis. BMC Genomics, Vol. 19, 478 p. DOI:10.1186/s12864-018-4878-3.
  19. Pol, M., Ruegg, P.L. (2019). Treatment practices and antibiotic usage for mastitis. Prev. Vet. Med., Vol. 165, pp. 102–112. DOI:10.1016/j.prevetmed.2019.02.010.
  20. Barkema, H.W., Green, M.J., Bradley, A.J. (2022). Role of S. aureus in bovine mastitis pathogenesis. Front. Vet. Sci., Vol. 9, 871 p. DOI:10.3389/fvets.2022.00871.
  21. Leelahapongsathon, K., Suriyasathaporn, W. (2020). New diagnostic approaches for subclinical mastitis. Animals (Basel), Vol. 10 (11), 2056 p. DOI:10.3390/ani10112056.
  22. Tomazi, T., Rossi, R.S., Gonçalves, J.L. (2021). Use of PCR for rapid detection of mastitis pathogens. J. Dairy Sci., Vol. 104 (2), pp. 1906–1916. DOI:10.3168/jds.2020-18876.
  23. Fadlelmoula, A., Nasr, M.A. (2019). Influence of management factors on mastitis prevalence. Trop. Anim. Health Prod., Vol. 51 (4), pp. 855–862. DOI:10.1007/s11250-018-1772-y.
  24. Gomes, F., Henriques, M. (2016). Control of bovine mastitis: old and recent therapies. Front. Microbiol., Vol. 7, 272 p. DOI:10.3389/fmicb.2016.00272.
  25. Zemanová, M., Langová, L., Novotná, I. (2022). Immune mechanisms and resistance genes in prevention of mastitis. Arch. Anim. Breed., Vol. 65 (4), pp. 371–382. DOI:10.5194/aab-65 -371-2022.
  26. Kerro Dego, O., Vidlund, S. (2024). Staphylococcal mastitis in dairy cows: epidemiology and control. Front. Vet. Sci., Vol. 11, 1042 p. DOI:10.3389/fvets.2024.01042.
  27. Benitez-Cabello, A., Morales, P., Rodriguez-Maresca, M. (2023). Detection of mastitis pathogens by real-time PCR. Pathogens, Vol. 12 (6), 812 p. DOI:10.3390/pathogens12060812.
  28. Tong, X., Barkema, H.W., Nobrega, D.B., Xu, C., Han, B., Zhang C., Yang, J., Li, X., Gao, J. (2025). Virulence of Bacteria Causing Mastitis in Dairy Cows: A Literature Review. MDPI Microorganisms, Vol. 13, no. 1, 167 p. DOI:10.3390/microorganisms13010167.
  29. Cunha, M.L.R.S., Silva, L.A., Mendonça, E.C. (2022). Antimicrobial susceptibility of mastitis pathogens from Brazilian dairy herds. Brazilian J. Microbiol., Vol. 53 (2), pp. 1133–1144. DOI:10.1007/s42770-021-00678-4.
  30. Nashwa, M., El-Hofy, H., Ibrahim, A. (2025). Genetic determinants of antimicrobial resistance in bovine mastitis isolates. Veterinary World, Vol. 18 (2), pp. 225–234. DOI:10.14202/ vetworld.2025.225-234
  31. National mastitis council. Laboratory handbook on bovine mastitis. (2017). Madison (WI): National mastitis counil.
  32. Vasylkiv, O., Kukhtin, M. (2024). Identification of mastitis pathogens in cows. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Veterinary Sciences, Vol. 26, no. 115, pp. 51–56. DOI:10.32718/nvlvet11507. (In Ukrainian).
  33. Radzikhovsky, N., Deishkan, O. (2023). Methods for diagnosing infectious mastitis in cattle. Scientific and Technical Bulletin of State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives and Institute of Animal Biology, Vol. 24, no. 1, pp. 157–162. DOI:10.15421/scivp24123. (In Ukrainian).
  34. Horiuk, Y., Kukhtyn, M., Perkiy, Y., Horiuk, V. (2018). Distribution of main pathogens of mastitis in cows on dairy farms in the western region of Ukraine. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Veterinary Sciences, Vol. 20, no. 83, pp. 115–119. DOI:10.15421/nvlvet8322.
  35. Vasylkiv, O., Kukhtyn, M. (2024). Identification of causative agents of cow mastitis in farms of Ternopil region. Scientifi c Messenger of LNU of Veterinary Medicine and Biotechnologies. Veterinary Sciences, Vol. 26, no. 115, pp. 51–56. DOI:10.32718/nvlvet11507.
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Scientific Journal of Veterinary Medicine 1'2026