You are here

Antigenic activity of experimental series of inactivated vaccines against highly pathogenic avian influenza with dif-ferent levels of hemagglutinins

In the results of studies it was examined antigenic activity of experimental series of inactivated vaccine against highly pathogenic avian influenza with different levels of hemagglutinin. The dependence of the antigenic properties of the vaccine on the hemagglutinin levels in inactivated antigens, which are part of the vaccine was established. Thus, in chickens vaccinated twice with vaccines against highly pathogenic avian influenza with hemagglutinin level since 1:512 to 1:128, the average level of antibodies was 9,0 ± 0,77 – 7,66 ± 0,44 log2 in 30 days after vaccination. At the same time using the vaccine for the vaccination of chickens with the hemagglutinin level 1:64 average antibody level was 5,0 ± 1,41 log2. It was determined that the hemagglutinin level in inactivated antigens for vaccines should not be lower then 1:512, and optimal titer is 1:1024–1:2048, which can provide a high level of protection at vaccinated birds.

Key words: highly pathogenic avian influenza, vaccine, specific antibody level, the activity of inactivated antigens.

1. Capua I. Vaccination for notifiable avian influenza in poultry [Text] / I. Capua // Rev Sci Tech. – 2007. – Vol. 26, № 1. – P. 217-227.

2. Capua I. Ecology, epidemiology and human health implications of avian influenza viruses: why do we need to share genetic data? [Text] / I. Capua, D.J. Alexander // Zoonoses Public Health. – 2008. – Vol.55, № 1. – P. 2-15.

3. Genetic characterization of the NS gene indicates co-circulation of two sub-lineages of highly pathogenic avian influenza virus of H5N1 subtype in Northern Europe in 2006 [Text] / S. Zohari [et al.] // Emerg Infect Dis. – 2006. – Vol. 12, № 7. – P. 1167-1169.

4. H5N1 Influenza Virus, Domestic Birds, Western Siberia, Russia [Text] / A.M. Shestopalov [et al.] // Virus Genes. – 2008. – Vol. 36, № 1. – P. 117-125.

5. The definition of avian influenza and the use of vaccination against avian influenza. Sanco/B3/AH/R17/2000 [Text] / European Commission 2000 // Diseases of Poultry / Y. M. Saif. – John Wiley & Sons, 2011. – P. 134.

6. Villareal C. L. The Mexican avian influenza (H5N2) outbreak [Text] / C. L. Villareal, A. O. Flores // Proceedings of the fourth international symposium on avian influenza / D. E. Swayne, R. D. Slemons (eds.) ; U.S. Animal Health Association. – Richmond, VA, 1998. – Р. 18–22.

7. Outbreaks of low pathogenicity avian influenza in U.S.A. [Text] / D. A. Halvorson [at al.] // Proceedings of the fourth international symposium on avian influenza / D. E. Swayne, R. D. Slemons (eds.); U.S. Animal Health Association. – Richmond, VA, 1998. – Р. 36–46.

8. Avian influenza [Text] / ed. D. E. Swayne. – Bleckwell Publisning, 2008. – 605 p.

9. Vysokopatogennyj gryp ptyci [Tekst] / B. T. Stegnij [ta in.]. – H.: TOV «Povnokolir», 2006. – 144 s.

10. OIE Manual for Diagnostic Tests and Vaccines for Terrestrial Animals [Elektr. resurs] / Sposib dostupu: http://www.oie.int. – Zagolovok z ekranu.

AttachmentSize
PDF icon 2013_11_muzyka_ua.pdf261.53 KB