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Effect of the Hep-A-Stress drug on the metabolism of chicken broilers

The purpose of research. To study the influence of veterinary drug Hep-A-Stres (oral solution, manufactured by O.L.KAR, Shargorod) on the metabolism of chicken broilers.

Materials for the study were 40 Cobb-500 crossbred chickens, divided into 20 groups (control and experimental) of 20 each. Blood was taken at 29-day-old chicken broilers before the start of the experiment and at 41 days of age. The drug was dosed with water at a rate of 1 ml per 1 liter of drinking water for 10 days.

After application of the drug, the content of total protein in the blood serum of broiler chickens in the experimental group increased by 16,9 %, compared with the initial, the control observed the opposite trend. In broilers of the experimental group, the protein was more than 12,2 %, but because the limits of individual parameters were too broad in both groups (37,4–60,4 g/l – in the experimental group and 34,3–52,4 g/l – Control), the difference between the groups was unlikely.
A similar tendency was observed in the number of albumins – their share in the control group was 33,0±1,66%, experimental – 30,4±1,42 % (p<0,5) and immunoglobulins.

An important indicator of protein metabolism is uric acid, since an increase in its level is an indicator of the development of urinary diathesis. In the broilers of the control group, its content was significant and was in the 41-day age of 0,67±0,07 mmol/l. In the experimental group, the probable decrease, compared to the initial indicator, to the uric acid content to 0,36±0,04 mmol/l (p<0,001). The difference between group indices for 41-days is also likely (p<0,01).

An important indicator of the state of the liver is the activity of cellular enzymes – aminotransferases. At the beginning of the experiment, the activity of AsAT in the control and experimental groups did not differ (p<0,5) and was respectively 3,97±0,28 and 3,7±0,27 mmol/h•l. In the 41–day–old broilers of the control group, the activity of the enzyme did not change (3,90±0,18, p<0,5), and the experimental had a marked tendency to decrease (3,28±0,08 mmol/h•l; 11,4%; p1<0,1). The difference between the activity of AsAT in the broilers of the experimental and control groups was probable (p2<0,01) and was 15,9 %.

An important indicator of lipid metabolism, in which the liver is actively involved, is the level of cholesterol. In 29-day broilers, the content of cholesterol in both groups did not differ: in the control group – 6,48±0,82 mmol/l, experimental – 6,30±0,63 (p<0,5). After application, the cholesterol content in broilers in the experimental group decreased (p<0,01) to 3,7±0,23 mmol/l, while the control showed only a slight decrease (p<0,5), ie the difference was unlikely. In the broiler cholesterol, the experimental group was less on 27,2 % than in the control (5,08±0,24 mmol/l), and the difference between them was probable (p<0,01).

Significant changes in the activity of alkaline phosphatase. Its activity decreased: changes in the experimental group were probable (p<0,01) – the activity of the enzyme was 367±33,2 Od/l, and only the tendency to decrease (p<0,1) was established in the control.

The content of retinol (vitamin A) in blood serum in broilers of the experimental group increased, compared with the initial one, by 26,6 % (p2<0,05), the control remained stable (90,7±3,72 μg/100 ml, p3<0,5), but the final level of retinol in the experimental group was significantly (p2 <0,05) higher than in the control group, by 28,1 %.

Thus, the preparation Gep-A-Stres stimulates the albumin–assimilating function of hepatocytes, probably (p<0,001) decreases the level of uric acid (0,36±0,04 mmol/l) and cholesterol (3,7±0,23 mmol/l; p<0,01) in serum and reduces (3,28±0,08; p<0,01) activity of indicator for hepatocytes of the enzyme – ALAT. These changes are the criteria for hepatoprotective properties of the drug for broiler chickens.

The prospect of further research is the study of the effect of the Gep-A-Stress drug on the metabolism of chicken broilers for the prevention of post-vaccine stress.

Key words: Gep-A-Stres, total protein, albumin, uric acid, AsAT, ALAT, vitamin A, cholesterol, calcium, phosphorus, alkaline phosphatase, chicken broilers.

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