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Головна » Scientific Journal of Veterinary Medicine 2'2021

Serological monitoring of brucellosis among wild boars in Ukraine during 2019–2020

The key to the successful development of animalhusbandry is the epizootic welfare of the country. Forimplementation the set of preventive measures it is requiresa perfect system for diagnosing infectious diseases, includingbrucellosis. Despite the fact that Ukraine is consideredofficially free from this zoonosis among farm animals, thesituation regarding it in the wild fauna of our country remainsinsufficiently studied.This article presents the results of serological studies of548 sera samples of wild boars from 16 regions of Ukraine,collected during 2019 – 2020. The research was conductedon the basis of the State Scientific and Research Institute ofLaboratory Diagnostics and Veterinary and Sanitary Expertise(SSRILDVSE, Kyiv, Ukraine) by using the complementfixation test (CFT) and enzyme-linked immunosorbent assay(ELISA). In addition, all positive samples were further testedon yersiniosis with differential purposes.The systematization of obtained results show thatantibodies to Brucella spp. in sera samples from wild boarsin Ukraine were detected by CFT in 57 samples (10.4 % ofthe total number), by ELISA – in 83 (15.1 %). In general,antibodies to these pathogens were diagnosed in samplesfrom 13 regions (most often from Zhytomyr, Lviv andChernihiv).Positive samples by CFT and/or ELISA werefurther tested for the presence of antibodies to Yersiniaenterocolitica because, according to the literature andguidelines for diagnostic kits, possibility of crossreactions between brucellosis and yersiniosis. As a resultof differential studies, from the 83 positive on brucellosissamples, 42 also showed antibodies to Y. enterocolitica.Taking into account the cross-reactions between tests forbrucellosis and yersiniosis, antibodies to Brucella spp.during the analyzed period were found in 7.5 % of animals(in 41 of 548 samples).The prospect of this work is to further studies of theepizootic situation regarding brucellosis in the wild faunaof Ukraine. The obtained results indicate the circulationof antibodies to this zoonosis among wild boars in ourcountry.

Key words: Brucella spp., Yersinia enterocolitica,zoonosis, serological studies, CFT, ELISA, wild fauna.

 

ALIEKSEIEVA H. https://orcid.org/0000-0001-6158-5960


PISKUN A. https://orcid.org/0000-0002-9058-098X


PIANKIVSKA I. https://orcid.org/0000-0001-7499-4763


POLISHCHUK O. https://orcid.org/0000-0002-4415-2773


MIETOLAPOVA H. https://orcid.org/0000-0001-7188-9300


  1. OIE. (2019). Brucellosis (Brucella abortus, B. melitensis and B. suis) (Infection with B. abortus, B. melitensis andB. suis), in (ed.) Manual of Diagnostic Tests and Vaccines forTerrestrial Animals: World Organization for Animal Health,pp. 355-398. Available at:www.oie. int/fileadmin/Home/eng/Health_ standards/tahm/3.01.04_BRUCELLOSIS.pdf
  2. Korniienko, L.Y. (2008). Khronichni infektsijni khvoroby tvaryn [Chronic infectious diseases in animals]. Bila Tserkva: BNAU, 348 p. Available at:rep.btsau.edu.ua/bitstream/BNAU/ 1294/2/Hron%D1%96chn%D1%96_%D1%96nfekc%D1%96jn%D1%96_hvorobi_tvarin.pdf
  3. Olsen, S.C., Garin-Bastuji, B., Blasco, J.M., Nicola, A.M., Samartino, L. (2019). Brucellosis, in Stevenson,G.W. (ed.) Diseases of Swine 11th Edition. USA: Wiley-Blackwell, pp. 697–708. DOI:10.1002/9781119350927.ch50
  4. Corbel, M.J. (2006) Brucellosis in humans and animals, in (ed.) World Health Organization, Food and Agriculture Organization and World Organization for AnimalHealth. Switzerland: WHO Library Cataloguing-in-Publication Data. 89 p. Available at:apps.who.int/ iris/handle/10665/43597
  5. Algers, B., Blokhuis, H.J., Bøtner, A., Broom, D.M.,Costa, P., Domingo, M., Greiner, M., Hartung, J., Koenen, F.,Müller-Graf, C., Mohan, R., Morton, D.B., Osterhaus, A.,Pfeiffer, D.U., Roberts, R., Sanaa, M., Salman, M., Sharp,J.M., Vannier, P. and Wierup, M. (2009). Scientific opinion ofthe Panel of Animal Health and Welfare (AHAW) on Porcinebrucellosis (Brucella suis), on a request from the Commissionon porcine brucellosis (Brucella suis). EFSA Journal. Vol.1144, pp. 1–112. Available at:efsa.onlinelibrary.wiley.com/doi/pdf/10.2903/j.efsa. 2009.1144
  6. Lama, J.K., Bachoon, D.S. (2018). Detection ofBrucella suis, Campylobacter jejuni, and Escherichia coliStrains in Feral Pig (Sus scrofa) Communities of Georgia.Vector Borne Zoonotic Diseases. Vol. 18(7), pp. 350–355.DOI:10.1089/vbz.2017.2187
  7. Fredriksson-Ahomaa, M. (2019). Wild Boar: A Reservoir of Foodborne Zoonoses. Foodborne pathogens and disease. Vol. 16(3), pp. 153–165. DOI:10.1089/fpd.2018.2512
  8. Pierce, C.F., Brown, V.R., Olsen, S.C., Boggiatto, P., Pedersen, K., Miller, R.S., Speidel, S.E. and Smyser, T.J.(2020). Loci associated with antibody response in feralswine (Sus scrofa) infected with Brucella suis. Frontiersin veterinary science. Vol. 7, pp. 554–674. DOI:10.3389/fvets.2020.554674
  9. Artois, M., Depner, K., Guberti, V., Hars, J., Rossi, S.,Rutili, D. (2002). Classical swine fever (hog cholera) in wild boar in Europe. Scientific and Technical Review. Vol. 21, pp.287–304. DOI:10. 20506/rst.21.2.1332
  10. Rossi, S., Artois, M., Pontier, D., Crucière, C., Hars, J.,Barrat, J., Pacholek, X., Fromont, E. (2005). Long-term monitoring of classical swine fever in wild boar (Sus scrofa sp.)using serological data. Veterinary Research. Vol. 36, pp. 27–42. DOI:10.1051/vetres:2004050
  11. Ruiz-Fons, F., Segales, J., Gortazar, C. (2008). Areview of viral diseases of the European wild boar: effectsof population dynamics and reservoir role. Veterinary Journal. Vol. 176, pp. 158–169. DOI:10.1016/j.tvjl.2007.02.017
  12. Faber, M., Schink, S., Mayer-Scholl, A., Ziesch, C.,Schönfelder, R., Wichmann-Schauer, H., Stark, K. and Nöckler K. (2015). Outbreak of trichinellosis due to wild boar meatand evaluation of the effectiveness of post exposure prophylaxis, Germany, 2013. Clinical Infectious Diseases. Vol. 60,pp. 98–104. DOI:10.1093/cid/civ199
  13. Żmudzki, J., Jabłoński, A., Nowak, A., Zębek, S.,Arent, Z., Bocian, Ł., Pejsak Z. (2016). First overall reportof Leptospira infections in wild boars in Poland. Acta Veterinaria Scandinavica. Vol. 58, 3 p. DOI:10.1186/s13028-016-0186-7
  14. Cilia, G., Fratini, F., Turchi, B., Angelini, M., Cerri, D.,Bertelloni, F. (2021). Genital Brucella suis Biovar 2 Infectionof Wild Boar (Sus scrofa) Hunted in Tuscany (Italy). Microorganisms. Vol. 9, no. 3, 582 p. DOI:10.3390/microorganisms9030582
  15. Muñoza, P.M., Mickb, V., Sacchinic, L., Janowiczc, A.,de Miguela, M.J., Cherfab, M.-A., Nevadob, C.R., Giraultb, G.,Andrés-Barrancoa, S., Jayb, M., Di Giannatalec, E., Zillic, K.,Ancorac, M., Dondod, A., Zoppid, S., Arnale, M.C., Tittarellic, M., De Massisc, F., Garin-Bastujib, B., Blascoa, J.M. andGarofoloc, G. (2019). Phylogeography and epidemiology ofBrucella suis biovar 2 in wildlife and domestic swine. Veterinary Microbiology. Vol. 233, pp. 68–77. DOI:10.1016/j. vetmic.2019.04.025
  16. Instrukcija pro zahody z profilaktyky ta borot'by zbrucel'ozom tvaryn: zatv. nakazom Derzhavnogo departamentu veterynarnoi' medycyny Minagropromu Ukrai'ny vid25 sichnja 2000 r. № 4. [Instruction on measures for prevention and control of animal brucellosis: approved by the order of the State Department of Veterinary Medicine of theMinistry of Agriculture of Ukraine dated January 25, 2000 №4.]. Available at:https://zakon.rada.gov.ua/laws/show/z0135-00#Text
  17. Nastanova po diagnostyci brucel'ozu tvaryn: zatv.nakazom Derzhavnogo departamentu veterynarnoi' medycyny Minagropromu Ukrai'ny 10 ljutogo 1998 r. № 15–14/55.[Guidelines for the diagnosis of animal brucellosis: approvedby order of the State Department of Veterinary Medicine ofthe Ministry of Agriculture of Ukraine on February 10, 1998№ 15–14/55.].
  18. Martin, C., Pastoret, P.P., Brochier, B., Humblet, M.F.,Saegerman, C. (2011). A survey of the transmission of infectious diseases/infections between wild and domestic ungulates in Europe. Veterinary Research. Vol. 42(1), 70 p. DOI:10.1186/1297-9716-42-70
  19. Grantina-Ievina, L., Avsejenko, J., Cvetkova, S.,Krastina, D., Streikisa, M., Steingolde, Z., Vevere, I. andRodze, I. (2018). Seroprevalence of Brucella suis in easternLatvian wild boars (Sus scrofa). Acta Veterinaria Scandinavica. Vol. 60, no. 1, 19 p. DOI:10.1186/s13028-018-0373-9
  20. Lama, J.K., Bachoon, D.S. (2018). Detection of Brucella suis, Campylobacter jejuni, and Escherichia coli Strainsin Feral Pig (Sus scrofa) Communities of Georgia. Vectorborne and zoonotic diseases (Larchmont, N.Y.). Vol. 18(7),pp. 350–355. DOI:10.1089/vbz.2017.2187
  21. Szulowski, K., Iwaniak, W., Weiner, M., Złotnicka, J. (2013). Brucella suis biovar 2 isolations from cattlein Poland. Annals of Agricultural and Environmental Medicine. Vol. 20(4), pp. 672–675. PMID: 24364432
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Scientific Journal of Veterinary Medicine 2'2021