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INTERNATIONAL AND NATIONAL LEGISLATION TO CONTROL MICTOXINS IN FOOD: REVIEW

Today, the problem of monitoring mycotoxins has become global in connection with climate change, a violation of the ecological balance for the use of intensive technologies for processing crops, through air pollution and the accumulation of products of photochemical reactions (photooxidants), which leads to a decrease in plant resistance to phytopathogens.

Every year, the problem of mycotoxicosis is exacerbated, as toxic fungi adapt quickly to new technologies and modern plant protection products. The increase in mycotoxins in foods also relates to the widespread use of nitrogen fertilizers and pesticides.

Natural toxins create risks for the health of humans and animals, affect food security and nutrition, reducing people's access to healthy food. The World Health Organization is constantly appealing to national authorities to monitor and ensure that the levels of the most relevant natural toxins in foods are as low as possible and consistent with both national and international requirements.

Ukraine's membership in the WTO, an association with the European Union, and the expansion of international trade require solutions to the issues of free movement of goods, safe and healthy food, and, accordingly, an adequate level of protection of life and health of people. One of the most important ways to solve them is to improve and harmonize national food legislation in line with international standards, including on the control of mycotoxins.

The purpose of our work was to conduct an analysis of literary sources, international and national legislative acts on the control of mycotoxins in food products throughout the food chain.

To prepare the publication, we have used literary sources on the subject of publication, as well as we have conducted a comparative analysis of national and international legislative acts regulating procedures and methods for controlling the residues of mycotoxins in food.

An analysis of numerous sources has shown that the issue of monitoring mycotoxins in foods, improving laboratory control and risk-based approach to preventing foodborne mycotoxicosis worries scientists from different countries, including Ukrainian. The analysis of national legislation shows that national standards on maximum levels of pollutants have been revised in Ukraine and a number of standards have been harmonized for methods of monitoring the residues of mycotoxins in feed for productive animals, food products of animal and vegetable origin.

Key words: mycotoxins, food chain, food, international law, national legislation, control, safety, risks.

 

doi: 10.33245/2310-4902-2019-149-1-30-40

KHITSKA O. https://orcid.org/0000-0002-6937-230X


Gerard R.


1. Levitin, M.M. (2003). Mikotoksiny fitopatogennyh gribov i mikotoksikozy cheloveka [Mycotoxins of phytopathogenic fungi and human mycotoxicosis]. Uspehi med. Mikologii [Successes honey. mycology]. Vol. 1, рp. 148–150.

2. Atanda, S.A., Pessu, P.O., Agoda, S., Isong, I.U., Adekalu, O.A., Echendu, M.A., Falade T.C. (2011). Fungi and mycotoxins in stored foods. African Journal of Microbiology Research. Vol. 5(25). рp. 4373‒4382. Available at: http://www.academicjournals.org/AJMR DOI: 10.5897/AJMR11.487

3. Samuel, A.O. Adeyeye. (2016). Fungal mycotoxins in foods: A review. Cogent Food & Agriculture. Vol. 2, pp.  1213–1217. Available at:  http:// doi.org/10.1080/23311932.2016.1213127

4. Bayman, P., Baker, J.L. (2006). Ochratoxins: A global perspective. Mycopathologia. рp. 215–223. Available at:  http:// doi.org/10.1007/s11046-006-0055-4  

5. Ashiq, S. (2015). Natural occurrence of mycotoxins in food and feed: Pakistan perspective. Comprehensive Reviews in Food Science and Food Safety. Vol. 14, рp. 159–175. Available at: http://doi.org/10.1111/crf3.2015.14.issue-2

6. Duhnic'kij, V. B., Hmel'nic'kij, G. O., Bojko, G. V., Іshhenko, V. D. (2011). Veterinarna mіkotoksikologіja: navchal'nij posіbnik [Veterinary Mycotoxicology: Textbook]. Kyiv: Agrarian Education, 240 р.

7. Marin, S., Ramos, A.J., Cano-Sancho, G., Sanchis, V. (2013). Mycotoxins: Occurrence, toxicology, and exposure assessment. Food and Chemical Toxicology. Vol. 60, рp. 218‒237. Available at: https://doi.org/10.1016/j.fct.2013.07.047

8. Duhnic'kij, V. B. (2003). Negativnij vpliv niz'kih doz T-2 toksinu na organіzm tvarin [Negative effect of low doses of T-2 toxin on the organism of animals]. Bulletin of Agrarian Science. рp. 39–41.

9. Golovchak, N. (2007). Struktura ta vpliv mіkotoksinіv na zhivі mіkroorganіzmi [Structure and influence of mycotoxins on living microorganisms]. Herald of the University of Lviv: Biological Series. Issue 43, рp. 33–47.

10. Małgorzata, Piotrowska., Katarzyna, Śliżewska, Joanna, Biernasiak. (2013). Mycotoxins in Cereal and Soybean-Based Food and Feed: book. 47 р. Available at: https:// doi.org/10.5772/54470.

11. Furat, І.M., Ostapjuk, N.A., Antonjuk, M.Z. (2017). Bіologіchnі osoblivostі ta ekologіja predstavnikіv rodu Fusarium, zbudnikіv zahvorjuvan' zlakіv [Biological features and ecology of the genus Fusarium, the causative agents of cereal diseases]. Naukovі zapiski NaUKMA: Prirodnichі nauki [Scientific notes of NaUKMA: Natural sciences]. Vol. 197, рp. 3–18.

12. Antonissen, G., Martel, A., Pasmans, F., Ducatelle, R., Verbrugghe, E., Vandenbroucke, V., Li, S., Haesebrouck, F., Van Immerseel, F., Croubels, S. (2014). The impact of Fusarium mycotoxins on human and animal host susceptibility to infectious diseases. Toxins. Vol. 6, рp. 430–452. Available at: https://doi.org/10.3390/toxins6020430.

13. Barbosa, T. S., Pereyra, C. M., Soleiro, C. A., Dias, E. O., Oliveira, A. A., Keller, K. M.,  Rosa, C. A. R. (2013). Mycobiota and mycotoxins present in finished fish feeds from farms in the Rio de Janeiro State. Brazilian International Aquatic Research. 5, рp. 1–9. Available at: http://cursos.ufrrj.br/posgraduacao/ppgctia/wp-content/uploads/Carla%20S... 202008- 6970-5-3%202013.pdf;

14. Richard, J. L. (2007). Some major mycotoxins and their mycotoxicoses – An overview. International Journal of Food Microbiology. 119, рp. 3–10. Available at: https:// doi.org/10.1016/j.ijfoodmicro.2007.07.019.

15. Moss, M. O. (2008). Fungi, quality and safety issues in fresh fruits and vegetables. Journal of Applied Microbiology. Vol. 104, pp. 1239–1243. Available at: https://doi.org/10.1111/j.1365-2672.2007.03705.x;

16. Trucksess, M.W., Scott, P.M. (2008). Mycotoxins in botanicals and dried fruits: A review. Food Additives & Contaminants: Part A. 25, рp. 181–192. Available at: https:// doi.org/10.1080/02652030701567459.

17. Wood, G. E. (1992). Mycotoxins in foods and feeds in the United States. Journal of Animal Science. 70, рp. 3941–3949. Available at: https:// doi.org/10.2527/1992.70123941x.

18. Castegnaro, M., McGregor, D. (1998). Carcinogenic risk assessment of mycotoxins. Revue Méd. Vét. рp. 671‒678.

19. Evaluation of the increase of risk for public health related to a possible temporary derogation from the maximum level of deoxynivalenol, zearalenone and fumonisins for maize and maize products. European Food Safety Authority (EFSA) Journal. Parma, Italy. 2014, vol. 12(5):3699, 61 р. Available at: www.efsa.europa.eu/efsajournal.

20. Walker, R. (2002). Risk assessment of ochratoxin: current views of the European Scientific Committee on Food, the JECFA and the Codex Committee on Food Additives and Contaminants. Adv. Exp. Med. Biol. Vol. 504, pp. 249–255.

21. Tarasenko, G.P., Dejneka, S.Є., Turash, M.M., Blіnder, O.O., Blіnder, O.V. (2013). Do pytannja reglamentacii' vmistu mikotoksyniv u sojevyh produktah [On the regulation of mycotoxins in soy products]. Problemi harchuvannja [Food problems]. Issue 2, рp. 55–59.

22. World Health Organization. Mycotoxins in African foods: Implications to food safety and health. AFRO Food safety (FOS). Issue No. July 2006.

23. Gong, Y., Hounsa, A., Egal, S., Turner, P. C., Sutcliffe, A. E., Hall, A. J., Wild, C. P. (2004). Postweaning exposure to aflatoxin results in impaired child growth: A longitudinal study in Benin, West Africa. Environmental Health Perspectives. Vol. 112, рp. 1334–1338. Available at: http:// doi.org/10.1289/ehp.6954

24. Williams, J. H., Phillips, T. D., Jolly, P. E., Stile, J. K., Jolly, C. M., Aggarwal, D. (2004). Human aflatoxicosis in developing countries: A review of toxicology, exposure, potential health consequences, and interventions. Journal of Clinical Nutrition. 80, рp. 1106–1122. PMID:15531656 Available at: http:// doi.org/10.1093/ajcn/80.5.1106

25. Khlangwiset, P., Shephard, G. S., Wu, F. (2011). Aflatoxins and growth impairment: A review. Critical Reviews in Toxicology. Vol. 41, рp. 740–755. Available at: http://dx.doi.org/10.3109/ 10408444.2011.575766.

26. Recommended practices for the prevention of mycotoxins in food, feed and their products. Food and Agriculture Organization of the United Nations. Rome. 1979. 79 p.

27. Code of practice for the prevention and reduction of mycotoxin contamination in cereals CAC/RCP 51-2003. Available at: http://www.fao.org/fao-who-codexalimentarius/codex-texts/codes-of-practi...

28. Hans, van Egmond. (2002). Worldwide regulations for mycotoxins FAO. Advances in Experimental Medicine and Biology. Vol. 504, рp. 257–269. Source: PubMed. Available at: http:// doi.org/ 10.1007/978-1-4615-0629-4_27  

29. Worldwide Regulations for mycotoxins in food and feed 2003: FAO Food and Nutrition Paper. Rome, 2004. Vol. 81, 171 р.

30. Peraica, M., Radic, B., Lucic, A., Pavlovic, M. (1999). Toxic effects of mycotoxins in humans. Bulletin of the World Health Organization. Vol. 77, рp. 113–115. PMCID: PMC2557730. Available at: https://www.ncbi.nlm.nih.gov/pmc/ articles/PMC2557730/.

31. Maximum level and sampling plan for total aflatoxins in peanuts intended for further processing. CODEX STAN 209-1999. Rev.1. 2001. 5 p.

32. Codex General Standard for Contaminants and Toxins in Food and Feed. Codex Standard 193–1995. Codex Alimentarius Commission (CAC). 2014. Accessed 23 April 2014. Available at: http://www.codexalimentarius.net.

33. European Commission. Commission Recommendation of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding (2006/576/EC). Official Journal of the European Union 2006. Vol. 229, pp. 7–9.

34. European Commission. Commission Recommendation of 27 March 2013 on the presence of T-2 and HT-2 toxin in cereals and cereal products (2013/165/EU). 2013. pp. 12–14. Accessed Aug. 15, 2016. Available at: http:// eur -lex .europa .eu/ legal -content/ EN/ TXT/ PDF/ ?uri= CELEX: 32013H0165 & from = EN.

35. Gautam, A. K., Gupta, H., Soni, Y. (2012). Screening of fungi and mycotoxins associated with stored rice grains in Himachal Pradesh. International Journal of Theoretical and Applied Science. Vol. 4, рp. 128–133. Available at: https://www.researchtrend.net/ijtas/ijtas_2012/20%20DR% 20AJAY%20GAUTAM.pdf.

36. Trucksess, M. W., Scott, P. M. (2008). Mycotoxins in botanicals and dried fruits: A review. Food Additives & Contaminants: Part A. Vol. 25, рp. 181–192. Available at: http:// doi.org/ 10.1080/02652030701567459

37. Kana, R.K., Gnonlonfin, B.G.J., Harvey, J., Wainaina, J., Wanjuki, I., Skilton, R.A., Teguia, A. (2013). Mycobiota and toxigenicity profile of Aspergillus flavus recovered from food and poultry feed mixtures in Cameroon. Journal of Animal and Poultry Sciences. Vol. 2, рp. 98–107. Available at: https://cgspace.cgiar.org/handle/10568/68354.

38. Adejumo, T.O., Adejoro, D.O. (2014). Incidence of aflatoxins, fumonisins, trichothecenes and ochratoxins in Nigerian foods and possible intervention strategies. Food Science and Quality Management. Vol. 31, рp. 127–146. Available at: https://www.academia.edu/13923373/ Incidence_of_aflatoxins_fumonisins_trichothecenes_and_ochratoxins_in_Nigerian_foods_ and_possible_intervention_strategies.

39. Martins, M.L., Martins, H. M., Bernardo, F. (2001). Aflatoxins in spices marketed in Portugal. Food Additives and Contaminants. Vol. 18, рp. 315–319. Available at: http:// doi.org/ 10.1080/02652030120041.

40. Yin, Y. N., Yan, L. Y., Jiang, J. H., Ma, Z. H. (2008). Biological control of aflatoxin contamination of crops. Journal of Zhejiang University Science B. Vol. 9, рp. 787–792. Available at: https:// doi.org/10.1631/jzus.B0860003.

41. Okşan, UCKUN., Işıl, VAR. (2014). Monitoring of Aflatoxins in Peanuts. Turkish Journal of Agricultural and Natural Sciences: Special Issue. Vol. 1, рp. 1310–1314. Available at: https://www.researchgate.net/publication/281811461_ Monitoring_of_Aflatoxins_in_Peanuts.

42. Dorner, J.W. (2008). Management and prevention of mycotoxins in peanuts. Food Additives and Contaminants. Vol. 25(2), pp. 203–208. Available at: https://doi.org/10.1080/02652030701658357.

43. Monographs on the Evaluation of Carcinogenic Risks to Humans. Some Traditional Herbal Medicines, Some Mycotoxins, Naphthalene and Styrene. Aflatoxins. International Agency for Research on Cancer (IARC). Lyon, France, 2002. рp. 171–300.

44. European Commission. Commission Regulation (EU) No165/2010 of 26 February 2010 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants infoodstuffs as regards aflatoxins. 2010. рp. 8–12. Accessed Aug. 15, 2016. Available at: http:// eur -lex .europa .eu/legal-content/ EN/TXT/PDF/?uri=CELEX: 32010R0165 & from = EN.

45. Myra Evelyn, Flores-Flores, Elena, González-Peñas. (2018). Short communication: Analysis of mycotoxins in Spanish milk. J. Dairy Sci. Vol. 101, рp. 113–117. Available at: https://doi.org/10.3168/jds.2017-13290

46. Maria, Helena Iha, Cynara, Baltazar Barbosa, Isaura, Akemi Okada, Mary, W. Trucksess. (2013). Aflatoxin M1 in milk and distribution and stability of aflatoxin M1 during production and storage of yoghurt and cheese. Food Control. Vol. 29, рp. 1‒6. Available at: https://doi.org/10.1016/j.foodcont.2012.05.058.

47. Marco, A. Jonker, Hans, P. van Egmond, Rainer, W. Stephany. (1999). Mycotoxins in food of animal origin: a review. Study as a result from decisions 96/519/EG and 98/587/EC as a part of the CRL duties and laid down in EU Council Directive 96/23/EC (Council of the EU 1996 b). 41 p. Available at: https://www.rivm.nl/bibliotheek/ digitaaldepot/389002_095.pdf

48. Robens, J.F., Richard, J.L. (1992). Aflatoxins in animal and human health. Rev Environ Contam. Toxicol. Vol. 127,  рp. 69–94. PMID:1631352

49. Stroj, A.M., Gladka, N.V. (2007). Toksykologichna harakterystyka ohratoksynu A ta aktual'nist' jogo reglamentacij v Ukrai'ni [Toxicological characteristic of ochratoxin A and relevance of its regulations in Ukraine]. Aktual'ni problemy toksykologii' [Actual problems of toxicology]. Bezpeka zhyttjedijal'nosti ljudyny : VIII mizhnarodna naukova-praktychna konferencija [Safety of Human Life: VIII International Scientific and Practical Conference].  38 p.

50. Ashiq, S. (2015). Natural occurrence of mycotoxins in food and feed: Pakistan perspective. Comprehensive Reviews in Food Science and Food Safety. Vol. 14, рp. 159–175. Available at:  http://dx.doi.org/10.1111/crf3.2015.14.issue-2;

51. Richard, J.L., Meerdink, G., Maragos, C.M., Tumbleson, M., Bordson, G., Rice, L.G., Ross, P.F. (1996). Absence of detectable fumonisins in the milk of cows fed Fusarium proliferatum (Matsushima) Nirenberg culture material. Mycopathologia. рp. 123‒126. Available at: https://link.springer.com/article/10.1007%2FBF00439124.

52. Prelusky, D.B., Scott, P.M., Trenholm, H.L., Lawrence, G.A. (1990). Minimal transmission of zearalenone to milk of dairy cows. J. of Environm. Science and Health. рp. 87‒103. Available at: https://doi.org/10.1080/ 03601239009372678

53. Manfoud, R., Maresca, M., Garmy, N., Fantini, J. (2002). The mycotoxin patulin alters the barrier function of the intestinal epithelium: mechanism of action of the toxin and protective effects of glutathione. Toxicol. And Appl. Pharmacol. Vol. 181, рp. 209–218. PMID:12079430

54. Nіkov, P.S., Buharbai'va, A.S., Sarbai'v, B.T. (1990). Pro zabrudnennja plodovo-ovochevoi' produkcіi' patulіnom u Kazahstanі [On contamination of fruit and vegetable production by patulin in Kazakhstan]. Pitannja harchuvannja [The issue of nutrition]. no 5, рp. 59–61.

55. Huang, L.C., Zheng, N.,  Zheng, B.Q.,  Wen, F.,  Cheng, J.B.,  Han, R.W.,  Xu, X.M.,  Li, S.L.,  Wang, J.Q. (2014). Simultaneous determination of aflatoxin M1, ochratoxin A, zearalenone and α-zearalenol in milk by UHPLC–MS/MS. Food Chem. рp. 242–249. Available at: https://doi.org/10.1016/j.foodchem.2013.09.047.

56. Smith, M. C., S. Madec E. Coton N. Hymery. (2016). Natural co-occurrence of mycotoxins in foods and feeds and their in vitro combined toxicological effects. Toxins (Basel), Vol. 8, 94 p.  Available at: https:// doi.org/10.3390/toxins8040094.

57. Wan, L.Y.M., Turner, P.C., El-Nezami, H. (2013). Individual and combined cytotoxic effects of Fusarium toxins (deoxynivalenol, nivalenol, zearalenone and fumonisins B1) on swine jejunal epithelial cells. Food Chem. Toxicol. Vol. 57, pp. 276–283. Available at: https:// doi .org/ 10 .1016/ j.fct .2013 .03 .034.

58. Derzhavnі gіgієnіchnі pravila і normi. Reglament maksimal'nih rіvnіv okremih zabrudnjujuchih rechovin u harchovih produktah. Zareєstrovano 18 travnja 2013. N 774/23306. [Chinnij vіd 2016-06-14]. [State hygiene rules and regulations. Regulation of maximum levels of certain pollutants in food products. Registered May 18, 2013 N 774/23306. Effective from 2016-06-14.] Verkhovna Rada of Ukraine. Available at:  https://zakon.rada.gov.ua/laws/show/z0774-13

59. Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union. L 364/5. 2006. 20 р.

60. Prіshhenko, O.V., Novozhic'ka, Ju.M. (2014). Porіvnjal'na harakteristika suchasnih metodіv viznachennja mіkotoksinіv [Comparative characteristics of modern methods for the determination of mycotoxins]. Veterinary biotechnology. рp. 86–90.

61. Ahmad, Alshannaq, Jae-Hyuk, Yu. (2017). Occurrence, Toxicity, and Analysis of Major Mycotoxins in Food. Int. J. Environmental Research and Public Health. Vol. 14, 632 р. Available at: https:// doi .org/ 10.3390/ijerph14060632.

62. Commission Regulation (EC) No 401/2006 of 23 February 2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in food stuffs. Official Journal of the European Union. L 70/12. 2006. 23 р.

63. Joint FAO/WHO Food standards programme. Report of the thirty-seventh session of the Codex Committee on methods of analysis and sampling. Sodex Alimentarius Commission: Thirty ninth Session. Rome, 2016. 67 p.

64. EN 14123:2007. Foodstuffs. Determination of aflatoxin B1 and the sum of aflatoxin B1, B2, G1 and G2 in hazelnuts, peanuts, pistachios, figs, and paprika powder. High performance liquid chromatographic method with post-column derivatisation and immunoaffinity column clean up. Publication Date 2007-12-01. ICS Code 67.050. Publisher: CEN. 32 р.

65. EN 14133: 2009. Foodstuffs. Determination of ochratoxin A in wine and beer. HPLC method with immunoaffinity column clean-up. Publication Date 2009-06-30. ISBN 978 0 580 64237 1. Publisher: BSI. 16 р.

66. EN 14352:2004. Foodstuffs. Determination of fumonisin B1 and B2 in maize based foods. HPLC method with immunoaffinity column clean up. Publication Date 2004-08-06. ISBN 0 580 44205 5. Publisher: BSI. 22 р.

67. EN 15829: 2010. Foodstuffs. Determination of ochratoxin A in currants, raisins, sultanas, mixed dried fruit and dried figs. HPLC method with immunoaffinity column cleanup and fluorescence detection. Publication Date 2010-02-28. ISBN 978 0 580 63017 0. Publisher: BSI. 18 р.

68. DSTU EN 12955-2001. Viznachennja aflatoksinu V1 ta sumi aflatoksinіv V1, V2, G1 ta G2 u zernovih kul'turah, fruktah іz tverdoju shkіrkoju ta pohіdnih vіd nih produktah. Metod visokoefektivnoi’ rіdinnoi’ hromatografіi’ za dopomogoju postkolonochnoi’ derivatizacіi’. Chinnij vіd 2003-07-01. [DSTU EN 12955-2001. Definition of Aflatoxin B1 and the sum of aflatoxins B1, B2, G1 and G2 in cereals, hard-shell fruits and their derivatives. Method of high-performance liquid chromatography by means of postcolonial derivatization. Effective from 2003-07-01.]. Kind. officer Kyiv: Derzh-spozhyvstandart of Ukraine, 2001. 14 р.

69. DSTU EN ISO 15141-1-2001. Vyznachennja ohratoksynu A v zerni ta produktah iz zernovyh kul'tur. Chastyna 1. Metod vysokoefektyvnoi' ridynnoi' hromatografii' z ochyshhennjam sylikagelem. Chynnyj vid 2003-07-01.  [DSTU EN ISO 15141-1-2001. Determination of ochratoxin A in grains and cereal products. Part 1. Method of high performance liquid chromatography with silica gel purification. Effective from 2003-07-01]. Kind. officer Kyiv: Derzhspozhyvstandart of Ukraine, 2001. 12 р.

70. DSTU EN ISO 15141-2-2001. Vyznachennja ohratoksynu A u zerni ta produktah iz zernovyh kul'tur. Chastyna 2. Metod vysokoefektyvnoi' ridynnoi' hromatografii' z ochyshhannjam bikarbonatom. Chynnyj vid 2003-07-01. [DSTU EN ISO 15141-2-2001. Determination of ochratoxin A in grain and cereal products. Part 2. Method of high-performance liquid chromatography with bicarbonate purification. Effective from 2003-07-01]. Kind. officer Kyiv: Derzhspozhyvstandart of Ukraine, 2001. 14 р.

71. DSTU EN 13585:2009. Vyznachennja vmistu fumonizyniv B1 ta B2 u kukurudzi metodom VERH z ochyshhannjam tverdofaznoju ekstrakcijeju (EN 13585:2001, IDT). Chynnyj vid 2011-01-01. [DSTU EN 13585: 2009. Determination of the content of fumonisins B1 and B2 in corn by HPLC method with purification by solid phase extraction (EN 13585: 2001, IDT). Effective from 01/01/2011]. Kind. officer Kyiv: Derzhspozhyvstandart of Ukraine, 2001. 16 р.

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Scientific Journal of Veterinary Medicine 1'2019