You are here

Головна » 2020 » Scientific Journal of Veterinary Medicine 1'2020

The state of erythrocytopoiesis in dogs in physical and emotional load

In our studies, we examined the indices of erythrocytopoiesis in service dogs for physical activity. The studies were conducted on 1.5−2 years old Belgian Shepherd (Malinois) dogs who performed exercises from the international Mondioring program, which included physical and emotional training to train dogs for the Armed Forces, Border Troops and National Police. Blood was collected from animals before training (at rest) and after the training process (lasting 2 hours). It was found that 62.5 % of animals showed a tendency to increase the total number of erythrocytes and their populations − "old" and "mature". Such changes in dogs during exercise are indicative of more intense maturation of “young” erythrocytes in the peripheral blood, elimination of “mature” cells from the depot, increased resistance to the membrane of their membranes, and intensifi cation of the processes of erythrocyte attachment and delivery. With regard to hemoglobin content in the blood and hematocrit, these fi gures in 75.0 % of dogs after exercise tended to increase. However, red blood indices (MСН and MCV) tended to decrease, indicating an increase in the erythrocyte adaptation processes to physiological hypoxia during exercise and the appearance of a large number of unsaturated microcytic forms of erythrocytes in the bloodstream in particular. In the study of the ferumotransferrin complex, it should be noted that in 87.5 % of the animals, the amount of ferum, TIBC and transferrin levels increased, which are related to the release into the bloodstream of the reserve reserves of the ferrum for hemoglobin formation and adequate ability of hepatocytes to synthesize during transplantation.

Key words: dogs, physical activity, erythrocytes, hemoglobin, hematocrit, acid resistance of erythrocytes, ferrum, transferrin, ferumotransferin complex.

V. Golovakha https://orcid.org/0000-0001-5937-5075


Mostovoy E. https://orcid.org/0000-0002-2996-5936


О. Piddubnyak


Kurchev О.


  1. Catherine, OʼBriena, Larry, G. Berglundb. (2018). Predicting recovery from exertional heat strain in military working dogs. Journal of Thermal Biology. Vol. 76, pp. 45−51. Available at: https://doi.org/10.1016/j.jtherbio.2018.07.001
  2. Gazit, I., Terkel, J. (2003). Explosives detection by sniff er dogs following strenuous physical activity. Applied Animal Behaviour Science. Vol. 81, Issue 2, pp. 149−161. Available at: https://doi.org/10.1016/S0168-1591(02)00274-5 
  3. Kiley, M. Algya., Tzu-Wen, L. Cross., Kristen, N. (2018). Leuck. Apparent total-tract macronutrient digestibility, serum chemistry, urinalysis, and fecal characteristics, metabolites and microbiota of adult dogs fed extruded, mildly cooked, and raw diets. Journal of Animal Science. Vol. 96, Issue 9, pp. 3670–3683. Available at: https://doi.org/10.1093/jas/sky235
  4. Rizzo, M., Arfuso, F., Alberghina, D., Giudiceb, E., Gianesella, M., Piccionea, G. (2017). Monitoring changes in body surface temperature associated with treadmill exercise in dogs by use of infrared methodology. Journal of Thermal Biology. Vol. 69, pp. 64−68. Available at: https://doi.org/10.1016/j. jtherbio.2017.06.007
  5. Bouthegourd, J.C., Kelly, M., Clety, N., Tardif, S., Smeets, D. (2009). Eff ects of weight woss on heart  rate normalization and increase in spontaneous activity in moderately exercised overweight dogs. International Journal of Applied Research in Veterinary Medicine. Vol. 4, pp. 153−164.
  6. Holovakha, V.I., Anforova, M.V., Piddubniak, O.V., Dubovyi, A.A. (2015). Likuvannia hepatoanemichnoho syndromu u sluzhbovykh sobak [Treatment of hepatoanemic syndrome in service dogs]. Nauk. visnyk vet. Medytsyny [Scientifi c Bulletin of Veterinary Medicine]. Bila Tserkva, Vol. 2 (122), pp. 42−49.
  7. Anforova, M.V., Holovakha, V.I., Piddubniak, O.V., Tyshkivskyi, M.Ia. (2016). Zminy vlastyvostei erytrotsytiv u sobak [Changes in erythrocyte properties in dogs]. Nauk. visnyk Lviv. nats. un-tu vet. medytsyny ta biotekhnolohii im. S.Z. Hzhytskoho [Scientifi c Bulletin Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv]. Lviv, Vol. 18, no. 3 (71), Part 2, pp. 3–6.
  8. Holovakha, V.I., Piddubnyak, O.V., Bakhur, T.I., Vovkotrub, N.V., Antipov, A.A., Anfi orova, M.V., Gutyj, B.V., Slivinska, L.G., Kurdeko, O.P., Macynovich, A.O. (2018). Changes of erythrocytopoesis indices in dogs with babesiosi. Regulatory Mechanisms in Biosystems. no. 9(3), pp. 379–383. Available at:https://doi.org/10.15421/021856
  9. Stohrer, M., Hammer, B., Hammer, R., Brincker, B., Stangassinger, M. (2002). Oxidative stress following extreme physical stress. Part 1: Genesis, clinical relevance and studies on vitamin E-supplemented sled dogs. Tierarztliche Praxis Ausgabe Kleintiere Heimtiere. Vol. 30, Issue 4, pp. 266−273.
  10. Holovakha, V.Y., Sliusarenko, A.A., Svyrskaia, N.M., Mostovaia, A.V., Sliusarenko, S.V., Pyddubniak, O.V., Tushkyvskyi, M.Ia. (2018). Klynyko-hematolohycheskyi status u sobak y koshek pry atopycheskom dermatyte [Clinical and hematological status in dogs and cats with atopic dermatitis]. Uchenue zapysky UOV HAVM [Scientifi c notes UOV GAVM]. Vytebsk, Vol. 54, Issue 4, pp. 40–44.
  11. Bezukh, V.M., Piddubnyk, O.V. (2015). Kliniko-hematolohichnmi status sobak za otruiennia zookumarynom [Clinical-hematological status of dogs for zoocoumarin poisoning]. Nauk. visnyk vet. Medytsyny [Scientifi c Bulletin of Veterinary Medicine]. Bila Tserkva, Vol. 15 (118), pp.10−13.
  12. Menchetti, L.A., Guelfi , G.A., Speranza, R.B., Carotenuto, P.B., Moscati, L.C., Diverio, S.A. Benefi ts of dietary supplements on the physical fi tness of German Shepherd dogs during a drug detection training course. Public Library of Science. Vol. 14, Issue 6, June 2019. Available at: https://doi. org/10.1371/journal.pone.0218275
  13. Jahr, T.H.A., Fergestad, M.E.B., Brynildsrud, O.C., Brun-Hansen, H.A., Skancke, E.A. (2019). Haematological and serum biochemical values in Norwegian sled dogs before and after competing in a 600 km race. Acta Veterinaria Scandinavica. Vol. 61, Issue 1, Article 20.
  14. Syzova, Y.A., Kamenskaia, V.V., Fediakov, V.Y. (1980). Bezapparaturnyj sposob frakcionirovanija krasnyh kletok krovi v gradiente plotnosti saharozy [A non-apparatus method for fractionation of red blood cells in a sucrose density gradient]. Yzv. Syb. otd. AN SSSR [News Sib. Dep. USSR Academy of Sciences]. Vol. 3, no. 15, pp. 119–122.
  15. Levchenko, V.I., Holovakha, V.I., Kondrakhin, I.P. (2010). Metody laboratornoi klinichnoi diahnostyky khvorob tvaryn [Methods of laboratory clinical diagnosis of animal diseases].  Kyiv: Agricultural Education, 437 p.
  16. Jemerson, S.Dzh. (2000). Gemopojez. Razvitie kletok krovi [v kn.: Shiff man F.Dzh. Patofi ziologija krovi] [The development of blood cells [in the book: Shiff man F.J. Pathophysiology of blood]]. Moscow, St. Petersburg: BINOM − Nevsky dialect. pp. 17−42.
  17. Escudero, A., Montilla, J.C. Garcia, J.M. Sanchez-Quevedo, M.C. Periago, J.L., Hortelano, P., Suarez, M.D. (1998). Eff ect of dietary (n-9), (n-6) and (n-3) fatty acids on membrane lipid composition and morphology of fat erythrocytes. Biochimica et Biophysica Acta-lipids and Lipid Metabolism. Vol. 1394, Issue 1, pp. 65−73. Available at:https://doi. org/10.1016/S0005-2760(98)00095-2
  18. Roy, J. (2017). Shephard Peptic Ulcer and Exercise. Sports Medicine. Vol. 47, Issue 1, pp. 33–40.
  19. Vitger, A.D., Stallknecht, B.M., Miles, J.E., Hansen, S.L., Vegge, A., Bjornvad, C.R. (2017). Immunometabolic parameters in overweight dogs during weight loss with or without an exercise program. Domestic Animal Endocrinology. Vol. 59, pp. 58−66. Available at:https://doi.org/10.1016/j. domaniend.2016.10.007
  20. Spoo, J.W.A., Zoran, D.L.B., Downey, R.L.C., Bischoff , K.D., Wakshlag, J.J.E. (2015). Serum biochemical, blood gas and antioxidant status in search and rescue dogs before and after simulated fi eldwork. Veterinary Journal. Vol. 206, Issue 1, pp. 47−53. Available at:https://doi.org/10.1016/j. tvjl.2015.07.002
AttachmentSize
PDF icon golovaha_1_2020.pdf4.59 MB
2020
Scientific Journal of Veterinary Medicine 1'2020