You are here

Головна » 2020 » Scientific Journal of Veterinary Medicine 1'2020

Monitoring and diagnosis of poultry bacterial diseases in poultry farms of the Kyiv region

Bacterial safety in the modern poultry industry plays a key role and is one of the key factors in production effi ciency. In the structure of poultry infectious pathology, the leading place is occupied by such bacteria as Escherichia coli, Clostridium perfringens, Enterococcus cecorum, Staphylococcus aureus, Gallibacterium anatis. The results of studies of samples of pathological material obtained from a sick bird are presented that indicate that most often Escherichia coli cultures were isolated from the heart (41,5 %), liver (22,0 %) and lungs (20,7 %), less often spleen (5,2 %) and kidney (2,0 %). Most isolated cultures of Escherichia coli (78 %) caused hemolysis when plating material on blood agar. The largest number of pathogenic cultures of Escherichia coli was isolated from adult chickens, signifi cantly fewer chickens under the age of 20 days. The associated course of bacterioses caused by two or more pathogens was noted in 89,8 % of cases. In 38,5 % of cases, Escherichia coli, bacteria of the genus Staphylococcus and Gallibacterium anatis were isolated from pathological material, in 27,3 % – a joint course of escherichiosis, staphylococcosis and enterobacteriosis was noted, in 15,7 % – escherichiosis, salmonellosis and enterobacteriosis, in 8,3 % – pasteurellosis and enterobacteriosis. In 13,2 % of cases with pathological material from chickens (liver, joints, in chickens – blind processes of the intestine) Clostridium perfringens was isolated. Three species of bacteria of the genus Staphylococcus were identifi ed: Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus pluranimalium (51,7 % from the liver, 21,7 from the lungs, 18,3 from the spleen, 5,0 % from the kidneys). Enterococcus cecorum was identifi ed in 11,32 % of the studied samples from cloacal swabs, oviducts and bone marrow, and Gallibacterium anatis was identifi ed in 11,32 % (from the upper respiratory tract and genitals).

Key words: poultry farms, monitoring, bacterial infection, Escherichia coli, Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus pluranimalium, Enterococcus cecorum, Clostridium perfringens, Gallibacterium anatis. 

Tyshkivskaya N. https://orcid.org/0000-0003-4937-1390


LYASOTA V. https://orcid.org/0000-0002-2442-2174


TYSHKIVSKAYA A. https://orcid.org/0000-0003-4419-2174


BUKALOVA N. https://orcid.org/0000-0003-4856-3040


BOGATKO N. https://orcid.org/0000-0002-1566-1026


  1. Stehnii, B.T. (2013). Analiz epizootychnoho monitorynhu bakterialnykh zakhvoriuvan silskohospodarskoi, dykoi ta dekoratyvnoi ptytsi na terytorii Skhodu Ukrainy [Analysis of epizootic monitoring of bacterial diseases of agricultural, wild and ornamental birds in the East of Ukraine]. Vet. medytsyna: mizhvid. temat. nauk. zb. [Veterinary medicine: interdepartmental thematic scientifi c collection]. Issue 97, pp. 232–233.
  2. Current respiratory disease problem and the probes in chicken / S. Hasan et all. Pakistan Vet. J.  2002. Vol. 22, № 1.  P. 17–20.
  3. Plytov, Y.S. (2011). Indikacija patogennyh bakterij, cirkulirujushhih v pticevodcheskih hozjajstvah [Indication of pathogenic bacteria circulating in poultry farms]. Probl. vet. sanitarii, gigieny i jekologii [Problems of veterinary sanitation, hygiene and ecology]. no.1 (5), pp. 63–65.
  4. DSTU 4769 2007. Bakteriolohichni doslidzhennia patolohichnoho materialu vid tvaryn. Metody vyiavlennia salmonel. [Chynnyi vid 2009-01-01] [DSTU 4769 2007. Bacteriological studies of pathological material from animals. Methods for detecting salmonella. [Eff ective from 2009-01-01]]. Offi  cially issued. Kyiv: Derzhspozhyvstandart of Ukraine, 2007. 37 p.
  5. Bessarabov, V. F. (2007). Bolezny ptyts [Disease of birds]. St. Petersburg: Doe, 448 p.
  6. Opredelytel bakteryi Berdzhy: per. s anhl. / pod red. Dzh. Khulta [The determinant of bacteria, Bergee: translation from English / edited by J. Hult]. Moscow: World, 1997, 432 p.
  7. Plytov, Y.S. (2011). Indikacija patogennyh bakterij, cirkulirujushhih v pticevodcheskih hozjajstvah [Indication of pathogenic bacteria circulating in poultry farms]. Probl. vet. sanitarii, gigieny i jekologii [Problems of veterinary sanitation, hygiene and ecology]. no. 1 (5), pp. 63–65.
  8. Welker, M., Moore, E.R. (2011). Applications of whole-cell matrix-assisted laser-desorption/ionization timeof-fl ight mass spectrometry in systematic microbiology. Syst. Appl. Microbiol. no. 34, pp. 2–11. Availaible at:https:// doi.org/10.1016 / j.syapm.2010.11.013
  9. Clostridial Diseases of Animals / A. Francisco Uzal et all. John Wiley & Sons. 2016. 336 p.
  10. Zon, H.A., Sorokova, V.V. (2009). Patolohoanatomichnyi ta patomorfolohichnyi proiav khronichnoho perebihu nekrotychnoho enterytu u kurei [Pathologicalanatomical and pathomorphological manifestation of chronic necrotic enteritis in chickens]. Visnyk Poltavskoi derzhavnoi ahrarnoi akademii [Bulletin of the Poltava State Agrarian Academy]. no. 3, pp. 133–136.
  11. Sorokova, V.V., Zon, H.A. (2002). Vyvchennia patohennykh vlastyvostei kultur C. perfringens, izolovanykh vid kurei [Study of pathogenic properties of C. perfringens cultures isolated from chickens]. Visnyk Sumskoho NAU [Bulletin of Sumy National Agrarian University]. Issue 7, pp. 88–90.
  12. Zon, H.A., Sorokova, V.V. (2002). Morfolohichna reaktsiia orhaniv imunokompetentnoi systemy i nadnyrnykiv kurchat, infi kovanykh C. Perfringens [Morphological response of organs of the immunocompetent system and adrenal glands of chickens infected with C. perfringens]. Visnyk Sumskoho NAU [Bulletin of Sumy National Agrarian University]. Issue 8, pp. 34–36.
  13. Timbermontet, L. (2011). Necrotic enteritis in broilers: an updated review on the pathogenesis / Avian Pathology. Vol. 40 (4), pp. 341–347. Availaible at:https://doi. org/10,1080/ 03079457.2011.590967
  14. Infektsiini khvoroby ptytsi / za red. L. Ye. Korniienka [Infectious diseases of birds / ed. L. E. Kornienko]. Kherson: Green D.S, 2012, 528 p.
  15. Singh, S.V. (2016).  Gallibacterium anatis: an emerging pathogen of poultry birds and domiciled birds. J Veterinar Sci Techno. 324 p. Availaible at:https://doi. org/10,4172/2157-7579.1000324
  16. Andreeva, N.L. (2004). Izuchenie bakterial'nyh infekcij na pticefabrikah [The study of bacterial infections in poultry farms]. Veterinarija [Veterinary Medicine]. no. 5, pp. 14–16.
  17. Abe, Y. (2006). Encephalomalacia with Enterococcus durans infection in the brain stem and cerebral hemisphere in chicks in Japan.  Avian Dis. Vol. (50), pp. 139–141. Availaible at:https://doi.org/10,1637 / 7419-080805R.1
  18. Devriese, L.A. (1991). Composition of the enterococcal and streptococcal intestinal fl ora of poultry. Bacteriol, Vol. (71), pp. 46–50.
  19. Plys, V. M., Fotina, T. I. (2014). Monitorynh, klinichni oznaky ta patolohoanatomichni zminy za pasterelozu (kholery) ptytsi v asotsiatsiiakh z deiakymy infektsiinymy ta invaziinymy zakhvoriuvanniamy [Monitoring, clinical signs and pathological changes in avian pasteurellosis (cholera) in associations with certain infectious and invasive diseases]. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu [Bulletin of Sumy National Agrarian University]. Issue 6 (35), pp. 114–121.
  20. Fotina, H.A., Klishchova, Zh.Ye. (2016). Chutlyvist zbudnykiv bakterialnykh khvorob ptytsi do antybakterialnykh preparativ [Sensitivity of pathogens of bacterial diseases of poultry to antibacterial drugs]. Naukovyi visnyk LNUVMBT imeni S. Z. Gzhytskoho [Scientifi c Bulletin Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv]. Vol. 18, no. 3 (71), pp. 182–185.
  21. Bovkun, G.F. (2004). Rol' mikrofl ory pri zabolevaniyakh pishchevaritel'nogo trakta u tsyplyat [The role of microfl ora in diseases of the digestive tract in chickens]. Veterinariya [Veterinary Medicine]. no. 3, pp. 37–40.
  22. Lysko, S.B., Khat'ko, N.F., Suntsova, O.A. (2006). Chuvstvitel'nost' mikoplazm i esherikhiy k antibakterial'nym preparatam [The sensitivity of mycoplasmas and Escherichia to antibacterial drugs]. Veterinariya [Veterinary Medicine]. no. 3, pp. 31–32.
  23. Febler, A.T., Kadlec, K., Hauschild, M.H. (2011). Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Food and Food Products of Poultry Origin in Germany. Applied and Environmental Microbiology. Vol. 77, pp. 7151–7157.
  24. Hayes, J.R., English, L.L., Carr, L. E. (2004). Multipleantibiotic esistance of Enterococcus spp. isolated from commercial poultry production environments. Appl. Environ. Microbiol. Vol. 70 (10), pp. 6005–6011. Availaible at:https://doi.org/10,1128/AEM.70.10.6005–6011.2004
AttachmentSize
PDF icon tyshkivska_1_2020.pdf6.29 MB
2020
Scientific Journal of Veterinary Medicine 1'2020