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Sanetary and hygienic assessment of the two-spore mushroom (Agaricus bisporus) following treatment with cellulase and succinic acid solutions

The technological process of growing champignons includes four independent, interrelated technologies: preparation of substrate (compost), preparation of covering material, cultivation of planting material - mycelium (mycelium), cultivation of culture. The aim of the study was to substantiate the eff ectiveness of using a solution of cellulase and succinic acid in the technology of edible mushroom champignon two-spore (Agaricus bisporus). Materials and methods of research. The work was carried out on the basis of the departments of veterinary examination, hygiene of livestock products and pathoanatomy named after Y.S. Zagaevsky and Food Technologies and Processing of Livestock Products of Bila Tserkva National Agricultural University during 2024−2025. The following research methods were used: analytical, technological, chemical, hygienic and statistical. As a result of the research, it was found that the use of cellulase enzymes and succinic acid in the composition of irrigation water contributes to an increase in the hydrolysis of cellulose contained in the substrate and an increase in the transformation of nutrients from the substrate into the biomass of the edible mushroom Agaricus bisporus (Agaricus bisporus). A decrease in the content of mannan sugars in the biomass of the edible mushroom by 15.1–17.4 % was found, which prevent the absorption of nutrients from mushrooms in the human gastrointestinal tract due to the action of succinic acid. When using solutions of cellulases and succinic acid, the chemical indicators of the two-spore champignon are improved due to an increase in the content of: proteins by 12.5 %, carbohydrates – 9.0 % and fats – 9.0 % (p≤0.05). Irrigation of the champignon substrate with water containing 0.01 % cellulose and 0.01 % succinic acid increases the yield of mushrooms by 14.1 %. The greatest economic efficiency of the two-spore champignon technology under the action of cellulases and succinic acid was obtained when using fi ltered water containing 0.01 % cellulases and 0.01 % succinic acid. The cultivation effi ciency was 275.5 hryvnias per 9.84 kg per 1 m2, which is 34.2 hryvnias, or 14.2 % more than in the control variant. A method of using cellulase and succinic acid solutions using the technology of the two-spore mushroom (Agaricus bisporus) was developed and its effi ciency was calculated. Irrigation was carried out daily after the substrate was added to the boxes, as well as after the application of the covering material. In order to use the cellulase and succinic acid solution using the technology of the edible mushroom (Agaricus bisporus), it is recommended to develop methodological recommendations “Use of cellulase and succinic acid solution using the technology of the edible mushroom (Agaricus bisporus)”.

Keywords: edible mushroom (Agaricus bisporus), organoleptic, physical, chemical, hygienic, tech nological, microbiological indicators, safety, quality, cellulase and succinic acid solutions.

LYASOTA V. https://orcid.org/0000-0002-2442-2174


BOGATKO N. https://orcid.org/0000-0002-1566-1026


BUKALOVA N. https://orcid.org/0000-0003-4856-3040


BOGATKO A.


MAZUR T. https://orcid.org/0000-0002-9295-7787


KHITSKA O. https://orcid.org/0000-0002-6937-230X


DZHMIL V. https://orcid.org/0000-0003-3590-0167


PRYSYAZHNYUK T.


TKACHUK S. https://orcid.org/0000-0002-6923-1793


PRYLIPKO T. https://orcid.org/0000-0002-8178-20X


BARTKIV L. https://orcid.org/0009-0007-7045-6010


BOLOKHOVSKA V. https://orcid.org/0009-0005-2728-4589


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Scientific Journal of Veterinary Medicine 1'2026